Cargando…

Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99

Serotonin N-acetyltransferase (SNAT) catalyzes the biosynthesis of N-acetylserotonin (NAS) and N-acetyltryptamine (NAT), two pleiotropic molecules with neurotransmitter functions. Here, we report the identification of a SNAT protein in the genus Staphylococcus. The SNAT gene identified in Staphyloco...

Descripción completa

Detalles Bibliográficos
Autores principales: Hafza, Nourhane, Li, Ningna, Luqman, Arif, Götz, Friedrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9992809/
https://www.ncbi.nlm.nih.gov/pubmed/36910235
http://dx.doi.org/10.3389/fmicb.2023.1073539
_version_ 1784902399254593536
author Hafza, Nourhane
Li, Ningna
Luqman, Arif
Götz, Friedrich
author_facet Hafza, Nourhane
Li, Ningna
Luqman, Arif
Götz, Friedrich
author_sort Hafza, Nourhane
collection PubMed
description Serotonin N-acetyltransferase (SNAT) catalyzes the biosynthesis of N-acetylserotonin (NAS) and N-acetyltryptamine (NAT), two pleiotropic molecules with neurotransmitter functions. Here, we report the identification of a SNAT protein in the genus Staphylococcus. The SNAT gene identified in Staphylococcus pseudintermedius ED99, namely SPSE_0802, encodes a 140 residues-long cytoplasmic protein. The recombinant protein SPSE_0802 was expressed in E. coli BL21 and found to acetylate serotonin (SER) and tryptamine (TRY) as well as other trace amines in vitro. The production of the neuromodulators NAS and NAT was detected in the cultures of different members of the genus Staphylococcus and the role of SPSE_0802 in this production was confirmed in an ED99 SPSE_0802 deletion mutant. A search for SNAT homologues showed that the enzyme is widely distributed across the genus which correlated with the SNAT activity detected in 22 out of the 40 Staphylococcus strains tested. The N-acetylated products of SNAT are precursors for melatonin synthesis and are known to act as neurotransmitters and activate melatonin receptors, among others, inducing various responses in the human body. The identification of SNAT in staphylococci could contribute to a better understanding of the interaction between those human colonizers and the host peripheral nervous system.
format Online
Article
Text
id pubmed-9992809
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99928092023-03-09 Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99 Hafza, Nourhane Li, Ningna Luqman, Arif Götz, Friedrich Front Microbiol Microbiology Serotonin N-acetyltransferase (SNAT) catalyzes the biosynthesis of N-acetylserotonin (NAS) and N-acetyltryptamine (NAT), two pleiotropic molecules with neurotransmitter functions. Here, we report the identification of a SNAT protein in the genus Staphylococcus. The SNAT gene identified in Staphylococcus pseudintermedius ED99, namely SPSE_0802, encodes a 140 residues-long cytoplasmic protein. The recombinant protein SPSE_0802 was expressed in E. coli BL21 and found to acetylate serotonin (SER) and tryptamine (TRY) as well as other trace amines in vitro. The production of the neuromodulators NAS and NAT was detected in the cultures of different members of the genus Staphylococcus and the role of SPSE_0802 in this production was confirmed in an ED99 SPSE_0802 deletion mutant. A search for SNAT homologues showed that the enzyme is widely distributed across the genus which correlated with the SNAT activity detected in 22 out of the 40 Staphylococcus strains tested. The N-acetylated products of SNAT are precursors for melatonin synthesis and are known to act as neurotransmitters and activate melatonin receptors, among others, inducing various responses in the human body. The identification of SNAT in staphylococci could contribute to a better understanding of the interaction between those human colonizers and the host peripheral nervous system. Frontiers Media S.A. 2023-02-22 /pmc/articles/PMC9992809/ /pubmed/36910235 http://dx.doi.org/10.3389/fmicb.2023.1073539 Text en Copyright © 2023 Hafza, Li, Luqman and Götz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hafza, Nourhane
Li, Ningna
Luqman, Arif
Götz, Friedrich
Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99
title Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99
title_full Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99
title_fullStr Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99
title_full_unstemmed Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99
title_short Identification of a serotonin N-acetyltransferase from Staphylococcus pseudintermedius ED99
title_sort identification of a serotonin n-acetyltransferase from staphylococcus pseudintermedius ed99
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9992809/
https://www.ncbi.nlm.nih.gov/pubmed/36910235
http://dx.doi.org/10.3389/fmicb.2023.1073539
work_keys_str_mv AT hafzanourhane identificationofaserotoninnacetyltransferasefromstaphylococcuspseudintermediused99
AT liningna identificationofaserotoninnacetyltransferasefromstaphylococcuspseudintermediused99
AT luqmanarif identificationofaserotoninnacetyltransferasefromstaphylococcuspseudintermediused99
AT gotzfriedrich identificationofaserotoninnacetyltransferasefromstaphylococcuspseudintermediused99