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A Tumor-admixture Model to Interrogate Immune Cell–dependent Tumorigenesis

A rigorous determination of effector contributions of tumor-infiltrating immune cells is critical for identifying targetable molecular mechanisms for the development of novel cancer immunotherapies. A tumor/immune cell–admixture model is an advantageous strategy to study tumor immunology as the fund...

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Detalles Bibliográficos
Autores principales: Noe, Jordan T., Ding, Chuanlin, Geller, Anne E., Rendon, Beatriz E., Yan, Jun, Mitchell, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 202
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993080/
https://www.ncbi.nlm.nih.gov/pubmed/36908637
http://dx.doi.org/ 10.21769/BioProtoc.4630
Descripción
Sumario:A rigorous determination of effector contributions of tumor-infiltrating immune cells is critical for identifying targetable molecular mechanisms for the development of novel cancer immunotherapies. A tumor/immune cell–admixture model is an advantageous strategy to study tumor immunology as the fundamental methodology is relatively straightforward, while also being adaptable to scale to address increasingly complex research queries. Ultimately, this method can provide robust experimental information to complement more traditional murine models of tumor immunology. Here, we describe a tumor/macrophage-admixture model using bone marrow–derived macrophages to investigate macrophage-dependent tumorigenesis. Additionally, we provide commentary on potential branch points for optimization with other immune cells, experimental techniques, and cancer types.