Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection

In recent work we identified genes that confer the slow-growing and antibiotic-resistant small-colony variant (SCV) form of Staphylococcus aureus , as associated with the amount of capsule the bacteria produce. In this study we isolated a triclosan-resistant SCV (tr-SCV) and demonstrated that it pro...

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Autores principales: Altwiley, Dina, Brignoli, Tarcisio, Duggan, Seána, Massey, Ruth C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Microbial Virulence and Pathogenesis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993119/
https://www.ncbi.nlm.nih.gov/pubmed/36748621
http://dx.doi.org/10.1099/mic.0.001277
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author Altwiley, Dina
Brignoli, Tarcisio
Duggan, Seána
Massey, Ruth C.
author_facet Altwiley, Dina
Brignoli, Tarcisio
Duggan, Seána
Massey, Ruth C.
author_sort Altwiley, Dina
collection PubMed
description In recent work we identified genes that confer the slow-growing and antibiotic-resistant small-colony variant (SCV) form of Staphylococcus aureus , as associated with the amount of capsule the bacteria produce. In this study we isolated a triclosan-resistant SCV (tr-SCV) and demonstrated that it produces significantly less capsule, an effect that appears to be mediated at the transcriptional stage. As with other SCVs, we found that the tr-SCV produces less toxins, and when compared to both a capsule and an Agr mutant we found the tr-SCV to be significantly attenuated in an insect model of infection.
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spelling pubmed-99931192023-03-09 Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection Altwiley, Dina Brignoli, Tarcisio Duggan, Seána Massey, Ruth C. Microbiology (Reading) Microbial Virulence and Pathogenesis In recent work we identified genes that confer the slow-growing and antibiotic-resistant small-colony variant (SCV) form of Staphylococcus aureus , as associated with the amount of capsule the bacteria produce. In this study we isolated a triclosan-resistant SCV (tr-SCV) and demonstrated that it produces significantly less capsule, an effect that appears to be mediated at the transcriptional stage. As with other SCVs, we found that the tr-SCV produces less toxins, and when compared to both a capsule and an Agr mutant we found the tr-SCV to be significantly attenuated in an insect model of infection. Microbiology Society 2023-01-30 /pmc/articles/PMC9993119/ /pubmed/36748621 http://dx.doi.org/10.1099/mic.0.001277 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Microbial Virulence and Pathogenesis
Altwiley, Dina
Brignoli, Tarcisio
Duggan, Seána
Massey, Ruth C.
Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection
title Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection
title_full Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection
title_fullStr Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection
title_full_unstemmed Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection
title_short Triclosan-resistant small-colony variants of Staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a Galleria mellonella model of infection
title_sort triclosan-resistant small-colony variants of staphylococcus aureus produce less capsule, less phenol-soluble modulins, and are attenuated in a galleria mellonella model of infection
topic Microbial Virulence and Pathogenesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9993119/
https://www.ncbi.nlm.nih.gov/pubmed/36748621
http://dx.doi.org/10.1099/mic.0.001277
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