Recycling of modified H2A-H2B provides short-term memory of chromatin states

Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H...

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Autores principales: Flury, Valentin, Reverón-Gómez, Nazaret, Alcaraz, Nicolas, Stewart-Morgan, Kathleen R., Wenger, Alice, Klose, Robert J., Groth, Anja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9994263/
https://www.ncbi.nlm.nih.gov/pubmed/36750094
http://dx.doi.org/10.1016/j.cell.2023.01.007
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author Flury, Valentin
Reverón-Gómez, Nazaret
Alcaraz, Nicolas
Stewart-Morgan, Kathleen R.
Wenger, Alice
Klose, Robert J.
Groth, Anja
author_facet Flury, Valentin
Reverón-Gómez, Nazaret
Alcaraz, Nicolas
Stewart-Morgan, Kathleen R.
Wenger, Alice
Klose, Robert J.
Groth, Anja
author_sort Flury, Valentin
collection PubMed
description Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states.
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spelling pubmed-99942632023-03-09 Recycling of modified H2A-H2B provides short-term memory of chromatin states Flury, Valentin Reverón-Gómez, Nazaret Alcaraz, Nicolas Stewart-Morgan, Kathleen R. Wenger, Alice Klose, Robert J. Groth, Anja Cell Article Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states. Cell Press 2023-03-02 /pmc/articles/PMC9994263/ /pubmed/36750094 http://dx.doi.org/10.1016/j.cell.2023.01.007 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Flury, Valentin
Reverón-Gómez, Nazaret
Alcaraz, Nicolas
Stewart-Morgan, Kathleen R.
Wenger, Alice
Klose, Robert J.
Groth, Anja
Recycling of modified H2A-H2B provides short-term memory of chromatin states
title Recycling of modified H2A-H2B provides short-term memory of chromatin states
title_full Recycling of modified H2A-H2B provides short-term memory of chromatin states
title_fullStr Recycling of modified H2A-H2B provides short-term memory of chromatin states
title_full_unstemmed Recycling of modified H2A-H2B provides short-term memory of chromatin states
title_short Recycling of modified H2A-H2B provides short-term memory of chromatin states
title_sort recycling of modified h2a-h2b provides short-term memory of chromatin states
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9994263/
https://www.ncbi.nlm.nih.gov/pubmed/36750094
http://dx.doi.org/10.1016/j.cell.2023.01.007
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