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LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma

Integrins are closely related to the occurrence and development of tumors. ITGA8 encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1. Studies on the role of this gene in the occurrence and development of lung cancer are scarce. The examination of public databases revealed that ITGA...

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Autores principales: Li, Xuanguang, Zhu, Guangsheng, Li, Yongwen, Huang, Hua, Chen, Chen, Wu, Di, Cao, Peijun, Shi, Ruifeng, Su, Lianchun, Zhang, Ruihao, Liu, Hongyu, Chen, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995370/
https://www.ncbi.nlm.nih.gov/pubmed/36911684
http://dx.doi.org/10.3389/fimmu.2023.1096818
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author Li, Xuanguang
Zhu, Guangsheng
Li, Yongwen
Huang, Hua
Chen, Chen
Wu, Di
Cao, Peijun
Shi, Ruifeng
Su, Lianchun
Zhang, Ruihao
Liu, Hongyu
Chen, Jun
author_facet Li, Xuanguang
Zhu, Guangsheng
Li, Yongwen
Huang, Hua
Chen, Chen
Wu, Di
Cao, Peijun
Shi, Ruifeng
Su, Lianchun
Zhang, Ruihao
Liu, Hongyu
Chen, Jun
author_sort Li, Xuanguang
collection PubMed
description Integrins are closely related to the occurrence and development of tumors. ITGA8 encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1. Studies on the role of this gene in the occurrence and development of lung cancer are scarce. The examination of public databases revealed that ITGA8 expression was significantly lower in tumor tissue than that in normal tissue, especially in lung cancer, renal carcinoma, and prostate cancer. Survival analysis of patients with lung adenocarcinoma revealed that higher ITGA8 expression had better prognosis. ITGA8 was positively related to immune checkpoints and immunomodulators, whereas B cell, CD4+ T cell, CD8+ T cell, neutrophil, macrophage, and dendritic cell infiltration had the same correlation. Moreover, ITGA8 was negatively related to cancer stemness. We used an online database to predict the miRNAs and lncRNAs that regulate ITGA8 and obtained the regulatory network of ITGA8 through correlation analysis and Kaplan–Meier survival analysis. Quantitative real-time PCR and western blot analyses showed that LINC01798 regulates ITGA8 expression through miR-17-5p. Therefore, the regulatory network of ITGA8 may serve as a new therapeutic target to improve the prognosis of patients with lung cancer.
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spelling pubmed-99953702023-03-10 LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma Li, Xuanguang Zhu, Guangsheng Li, Yongwen Huang, Hua Chen, Chen Wu, Di Cao, Peijun Shi, Ruifeng Su, Lianchun Zhang, Ruihao Liu, Hongyu Chen, Jun Front Immunol Immunology Integrins are closely related to the occurrence and development of tumors. ITGA8 encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1. Studies on the role of this gene in the occurrence and development of lung cancer are scarce. The examination of public databases revealed that ITGA8 expression was significantly lower in tumor tissue than that in normal tissue, especially in lung cancer, renal carcinoma, and prostate cancer. Survival analysis of patients with lung adenocarcinoma revealed that higher ITGA8 expression had better prognosis. ITGA8 was positively related to immune checkpoints and immunomodulators, whereas B cell, CD4+ T cell, CD8+ T cell, neutrophil, macrophage, and dendritic cell infiltration had the same correlation. Moreover, ITGA8 was negatively related to cancer stemness. We used an online database to predict the miRNAs and lncRNAs that regulate ITGA8 and obtained the regulatory network of ITGA8 through correlation analysis and Kaplan–Meier survival analysis. Quantitative real-time PCR and western blot analyses showed that LINC01798 regulates ITGA8 expression through miR-17-5p. Therefore, the regulatory network of ITGA8 may serve as a new therapeutic target to improve the prognosis of patients with lung cancer. Frontiers Media S.A. 2023-02-23 /pmc/articles/PMC9995370/ /pubmed/36911684 http://dx.doi.org/10.3389/fimmu.2023.1096818 Text en Copyright © 2023 Li, Zhu, Li, Huang, Chen, Wu, Cao, Shi, Su, Zhang, Liu and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Li, Xuanguang
Zhu, Guangsheng
Li, Yongwen
Huang, Hua
Chen, Chen
Wu, Di
Cao, Peijun
Shi, Ruifeng
Su, Lianchun
Zhang, Ruihao
Liu, Hongyu
Chen, Jun
LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
title LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
title_full LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
title_fullStr LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
title_full_unstemmed LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
title_short LINC01798/miR-17-5p axis regulates ITGA8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
title_sort linc01798/mir-17-5p axis regulates itga8 and causes changes in tumor microenvironment and stemness in lung adenocarcinoma
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995370/
https://www.ncbi.nlm.nih.gov/pubmed/36911684
http://dx.doi.org/10.3389/fimmu.2023.1096818
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