Second harmonic generation microscopy: a powerful tool for bio-imaging

Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It al...

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Autores principales: Aghigh, Arash, Bancelin, Stéphane, Rivard, Maxime, Pinsard, Maxime, Ibrahim, Heide, Légaré, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995455/
https://www.ncbi.nlm.nih.gov/pubmed/36909955
http://dx.doi.org/10.1007/s12551-022-01041-6
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author Aghigh, Arash
Bancelin, Stéphane
Rivard, Maxime
Pinsard, Maxime
Ibrahim, Heide
Légaré, François
author_facet Aghigh, Arash
Bancelin, Stéphane
Rivard, Maxime
Pinsard, Maxime
Ibrahim, Heide
Légaré, François
author_sort Aghigh, Arash
collection PubMed
description Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It also provides an overview of SHG and advanced SHG imaging in neuroscience and microtubule imaging and how these methods can aid in understanding microtubule formation, structuration, and involvement in neuronal function. Finally, we offer a perspective on the future of these methods and how technological advancements can help make SHG microscopy a more widely adopted imaging technique.
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spelling pubmed-99954552023-03-10 Second harmonic generation microscopy: a powerful tool for bio-imaging Aghigh, Arash Bancelin, Stéphane Rivard, Maxime Pinsard, Maxime Ibrahim, Heide Légaré, François Biophys Rev Review Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It also provides an overview of SHG and advanced SHG imaging in neuroscience and microtubule imaging and how these methods can aid in understanding microtubule formation, structuration, and involvement in neuronal function. Finally, we offer a perspective on the future of these methods and how technological advancements can help make SHG microscopy a more widely adopted imaging technique. Springer Berlin Heidelberg 2023-01-19 /pmc/articles/PMC9995455/ /pubmed/36909955 http://dx.doi.org/10.1007/s12551-022-01041-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Aghigh, Arash
Bancelin, Stéphane
Rivard, Maxime
Pinsard, Maxime
Ibrahim, Heide
Légaré, François
Second harmonic generation microscopy: a powerful tool for bio-imaging
title Second harmonic generation microscopy: a powerful tool for bio-imaging
title_full Second harmonic generation microscopy: a powerful tool for bio-imaging
title_fullStr Second harmonic generation microscopy: a powerful tool for bio-imaging
title_full_unstemmed Second harmonic generation microscopy: a powerful tool for bio-imaging
title_short Second harmonic generation microscopy: a powerful tool for bio-imaging
title_sort second harmonic generation microscopy: a powerful tool for bio-imaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995455/
https://www.ncbi.nlm.nih.gov/pubmed/36909955
http://dx.doi.org/10.1007/s12551-022-01041-6
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