Cargando…

Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats

Several chemicals and medications induce cellular damage in various organs of the body by activating reactive substances’ metabolism leading to various pathological conditions including liver disease. In this study, we evaluated the prophylactic and curative effects of Carica papaya Linn. pulp water...

Descripción completa

Detalles Bibliográficos
Autores principales: Shaban, Nadia Zaki, Awad, Olfat M., Fouad, Ghada M., Hafez, Afaf M., Abdul-Aziz, Ahmed Alaa, El-Kot, Sarah M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995559/
https://www.ncbi.nlm.nih.gov/pubmed/36396758
http://dx.doi.org/10.1007/s11356-022-24083-5
_version_ 1784902851821043712
author Shaban, Nadia Zaki
Awad, Olfat M.
Fouad, Ghada M.
Hafez, Afaf M.
Abdul-Aziz, Ahmed Alaa
El-Kot, Sarah M.
author_facet Shaban, Nadia Zaki
Awad, Olfat M.
Fouad, Ghada M.
Hafez, Afaf M.
Abdul-Aziz, Ahmed Alaa
El-Kot, Sarah M.
author_sort Shaban, Nadia Zaki
collection PubMed
description Several chemicals and medications induce cellular damage in various organs of the body by activating reactive substances’ metabolism leading to various pathological conditions including liver disease. In this study, we evaluated the prophylactic and curative effects of Carica papaya Linn. pulp water extract (PE) against CCl(4)-induced rat hepatotoxicity. Five groups of rats were created, control, PE, CCl(4), (PE-CCl(4)): The rats were administered with PE pre and during CCl(4) injection, and (PE-CCl(4)-PE): The rats were administered with PE pre, during, and after CCl(4). The markers of oxidative stress (“OS”: oxidant and antioxidants), inflammation [nuclear factor-κB, tumor necrosis factor-α, and interleukin-6], fibrosis [transforming growth factor-β], and apoptosis [tumor suppressor gene (p53)] were evaluated. Additionally, liver functions, liver histology, and kidney functions were measured. Also, PE characterization was studied. The results showed that PE, in vitro, has a high antioxidant capacity because of the existence of phenolics, flavonoids, tannins, terpenoids, and minerals. Otherwise, the PE administration [groups (PE-CCl(4)) and (PE-CCl(4)-PE)] exhibited its prophylactic and therapeutic role versus the hepatotoxicity induced by CCl(4) where PE treatment improved liver functions, liver histopathology, and renal functions by decreasing oxidative stress, inflammation, fibrosis, and apoptosis induced by CCl(4). Our study elucidated that PE contains high amounts of phenolics, flavonoids, tannins, terpenoids, and ascorbic acid. So, PE exerted significant prophylactic and curative effects against hepatotoxicity induced by CCl(4). These were done by enhancing the markers of antioxidants and drug-metabolizing enzymes with reductions in lipid peroxidation, inflammation, fibrosis, and apoptosis. PE administration for healthful rats for 12 weeks had no negative impacts. Consequently, PE is a promising agent for the prohibition and therapy of the toxicity caused by xenobiotics.
format Online
Article
Text
id pubmed-9995559
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-99955592023-03-10 Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats Shaban, Nadia Zaki Awad, Olfat M. Fouad, Ghada M. Hafez, Afaf M. Abdul-Aziz, Ahmed Alaa El-Kot, Sarah M. Environ Sci Pollut Res Int Research Article Several chemicals and medications induce cellular damage in various organs of the body by activating reactive substances’ metabolism leading to various pathological conditions including liver disease. In this study, we evaluated the prophylactic and curative effects of Carica papaya Linn. pulp water extract (PE) against CCl(4)-induced rat hepatotoxicity. Five groups of rats were created, control, PE, CCl(4), (PE-CCl(4)): The rats were administered with PE pre and during CCl(4) injection, and (PE-CCl(4)-PE): The rats were administered with PE pre, during, and after CCl(4). The markers of oxidative stress (“OS”: oxidant and antioxidants), inflammation [nuclear factor-κB, tumor necrosis factor-α, and interleukin-6], fibrosis [transforming growth factor-β], and apoptosis [tumor suppressor gene (p53)] were evaluated. Additionally, liver functions, liver histology, and kidney functions were measured. Also, PE characterization was studied. The results showed that PE, in vitro, has a high antioxidant capacity because of the existence of phenolics, flavonoids, tannins, terpenoids, and minerals. Otherwise, the PE administration [groups (PE-CCl(4)) and (PE-CCl(4)-PE)] exhibited its prophylactic and therapeutic role versus the hepatotoxicity induced by CCl(4) where PE treatment improved liver functions, liver histopathology, and renal functions by decreasing oxidative stress, inflammation, fibrosis, and apoptosis induced by CCl(4). Our study elucidated that PE contains high amounts of phenolics, flavonoids, tannins, terpenoids, and ascorbic acid. So, PE exerted significant prophylactic and curative effects against hepatotoxicity induced by CCl(4). These were done by enhancing the markers of antioxidants and drug-metabolizing enzymes with reductions in lipid peroxidation, inflammation, fibrosis, and apoptosis. PE administration for healthful rats for 12 weeks had no negative impacts. Consequently, PE is a promising agent for the prohibition and therapy of the toxicity caused by xenobiotics. Springer Berlin Heidelberg 2022-11-17 2023 /pmc/articles/PMC9995559/ /pubmed/36396758 http://dx.doi.org/10.1007/s11356-022-24083-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Shaban, Nadia Zaki
Awad, Olfat M.
Fouad, Ghada M.
Hafez, Afaf M.
Abdul-Aziz, Ahmed Alaa
El-Kot, Sarah M.
Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
title Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
title_full Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
title_fullStr Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
title_full_unstemmed Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
title_short Prophylactic and curative effects of Carica papaya Linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
title_sort prophylactic and curative effects of carica papaya linn. pulp extract against carbon tetrachloride-induced hepatotoxicity in male rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995559/
https://www.ncbi.nlm.nih.gov/pubmed/36396758
http://dx.doi.org/10.1007/s11356-022-24083-5
work_keys_str_mv AT shabannadiazaki prophylacticandcurativeeffectsofcaricapapayalinnpulpextractagainstcarbontetrachlorideinducedhepatotoxicityinmalerats
AT awadolfatm prophylacticandcurativeeffectsofcaricapapayalinnpulpextractagainstcarbontetrachlorideinducedhepatotoxicityinmalerats
AT fouadghadam prophylacticandcurativeeffectsofcaricapapayalinnpulpextractagainstcarbontetrachlorideinducedhepatotoxicityinmalerats
AT hafezafafm prophylacticandcurativeeffectsofcaricapapayalinnpulpextractagainstcarbontetrachlorideinducedhepatotoxicityinmalerats
AT abdulazizahmedalaa prophylacticandcurativeeffectsofcaricapapayalinnpulpextractagainstcarbontetrachlorideinducedhepatotoxicityinmalerats
AT elkotsarahm prophylacticandcurativeeffectsofcaricapapayalinnpulpextractagainstcarbontetrachlorideinducedhepatotoxicityinmalerats