Cargando…

Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels

Human pancreatic islets transplantation is an experimental therapeutic treatment for Type I Diabetes. Limited islets lifespan in culture remains the main drawback, due to the absence of native extracellular matrix as mechanical support after their enzymatic and mechanical isolation procedure. Extend...

Descripción completa

Detalles Bibliográficos
Autores principales: Marchini, Amanda, Ciulla, Maria Gessica, Antonioli, Barbara, Agnoli, Alessandro, Bovio, Umberto, Visnoviz, Virginia, Bertuzzi, Federico, Gelain, Fabrizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995881/
https://www.ncbi.nlm.nih.gov/pubmed/36911193
http://dx.doi.org/10.3389/fbioe.2023.1105157
_version_ 1784902917911740416
author Marchini, Amanda
Ciulla, Maria Gessica
Antonioli, Barbara
Agnoli, Alessandro
Bovio, Umberto
Visnoviz, Virginia
Bertuzzi, Federico
Gelain, Fabrizio
author_facet Marchini, Amanda
Ciulla, Maria Gessica
Antonioli, Barbara
Agnoli, Alessandro
Bovio, Umberto
Visnoviz, Virginia
Bertuzzi, Federico
Gelain, Fabrizio
author_sort Marchini, Amanda
collection PubMed
description Human pancreatic islets transplantation is an experimental therapeutic treatment for Type I Diabetes. Limited islets lifespan in culture remains the main drawback, due to the absence of native extracellular matrix as mechanical support after their enzymatic and mechanical isolation procedure. Extending the limited islets lifespan by creating a long-term in vitro culture remains a challenge. In this study, three biomimetic self-assembling peptides were proposed as potential candidates to recreate in vitro a pancreatic extracellular matrix, with the aim to mechanically and biologically support human pancreatic islets, by creating a three-dimensional culture system. The embedded human islets were analyzed for morphology and functionality in long-term cultures (14-and 28-days), by evaluating β-cells content, endocrine component, and extracellular matrix constituents. The three-dimensional support provided by HYDROSAP scaffold, and cultured into MIAMI medium, displayed a preserved islets functionality, a maintained rounded islets morphology and an invariable islets diameter up to 4 weeks, with results analogues to freshly-isolated islets. In vivo efficacy studies of the in vitro 3D cell culture system are ongoing; however, preliminary data suggest that human pancreatic islets pre-cultured for 2 weeks in HYDROSAP hydrogels and transplanted under subrenal capsule may restore normoglycemia in diabetic mice. Therefore, engineered self-assembling peptide scaffolds may provide a useful platform for long-term maintenance and preservation of functional human pancreatic islets in vitro.
format Online
Article
Text
id pubmed-9995881
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99958812023-03-10 Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels Marchini, Amanda Ciulla, Maria Gessica Antonioli, Barbara Agnoli, Alessandro Bovio, Umberto Visnoviz, Virginia Bertuzzi, Federico Gelain, Fabrizio Front Bioeng Biotechnol Bioengineering and Biotechnology Human pancreatic islets transplantation is an experimental therapeutic treatment for Type I Diabetes. Limited islets lifespan in culture remains the main drawback, due to the absence of native extracellular matrix as mechanical support after their enzymatic and mechanical isolation procedure. Extending the limited islets lifespan by creating a long-term in vitro culture remains a challenge. In this study, three biomimetic self-assembling peptides were proposed as potential candidates to recreate in vitro a pancreatic extracellular matrix, with the aim to mechanically and biologically support human pancreatic islets, by creating a three-dimensional culture system. The embedded human islets were analyzed for morphology and functionality in long-term cultures (14-and 28-days), by evaluating β-cells content, endocrine component, and extracellular matrix constituents. The three-dimensional support provided by HYDROSAP scaffold, and cultured into MIAMI medium, displayed a preserved islets functionality, a maintained rounded islets morphology and an invariable islets diameter up to 4 weeks, with results analogues to freshly-isolated islets. In vivo efficacy studies of the in vitro 3D cell culture system are ongoing; however, preliminary data suggest that human pancreatic islets pre-cultured for 2 weeks in HYDROSAP hydrogels and transplanted under subrenal capsule may restore normoglycemia in diabetic mice. Therefore, engineered self-assembling peptide scaffolds may provide a useful platform for long-term maintenance and preservation of functional human pancreatic islets in vitro. Frontiers Media S.A. 2023-02-23 /pmc/articles/PMC9995881/ /pubmed/36911193 http://dx.doi.org/10.3389/fbioe.2023.1105157 Text en Copyright © 2023 Marchini, Ciulla, Antonioli, Agnoli, Bovio, Visnoviz, Bertuzzi and Gelain. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Marchini, Amanda
Ciulla, Maria Gessica
Antonioli, Barbara
Agnoli, Alessandro
Bovio, Umberto
Visnoviz, Virginia
Bertuzzi, Federico
Gelain, Fabrizio
Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
title Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
title_full Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
title_fullStr Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
title_full_unstemmed Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
title_short Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
title_sort long-term cultures of human pancreatic islets in self-assembling peptides hydrogels
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995881/
https://www.ncbi.nlm.nih.gov/pubmed/36911193
http://dx.doi.org/10.3389/fbioe.2023.1105157
work_keys_str_mv AT marchiniamanda longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT ciullamariagessica longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT antoniolibarbara longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT agnolialessandro longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT bovioumberto longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT visnovizvirginia longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT bertuzzifederico longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels
AT gelainfabrizio longtermculturesofhumanpancreaticisletsinselfassemblingpeptideshydrogels