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Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation
Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that support cell adhesion and proliferation. Here we investigate...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995891/ https://www.ncbi.nlm.nih.gov/pubmed/36911186 http://dx.doi.org/10.3389/fbioe.2023.1116917 |
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author | dos Santos, Ana Elisa Antunes Cotta, Tiago Santos, João Paulo Ferreira Camargos, Juliana Sofia Fonseca do Carmo, Ana Carolina Correia Alcântara, Erika Gabriele Alves Fleck, Claudia Copola, Aline Gonçalves Lio Nogueira, Júlia Meireles Silva, Gerluza Aparecida Borges Andrade, Luciana de Oliveira Ferreira, Roberta Viana Jorge, Erika Cristina |
author_facet | dos Santos, Ana Elisa Antunes Cotta, Tiago Santos, João Paulo Ferreira Camargos, Juliana Sofia Fonseca do Carmo, Ana Carolina Correia Alcântara, Erika Gabriele Alves Fleck, Claudia Copola, Aline Gonçalves Lio Nogueira, Júlia Meireles Silva, Gerluza Aparecida Borges Andrade, Luciana de Oliveira Ferreira, Roberta Viana Jorge, Erika Cristina |
author_sort | dos Santos, Ana Elisa Antunes |
collection | PubMed |
description | Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that support cell adhesion and proliferation. Here we investigated CA nanofibers, associated or not with a bioactive annatto extract (CA@A), a food-dye, as potential scaffolds for cultivated meat and muscle tissue engineering. The obtained CA nanofibers were evaluated concerning its physicochemical, morphological, mechanical and biological traits. UV-vis spectroscopy and contact angle measurements confirmed the annatto extract incorporation into the CA nanofibers and the surface wettability of both scaffolds, respectively. SEM images revealed that the scaffolds are porous, containing fibers with no specific alignment. Compared with the pure CA nanofibers, CA@A nanofibers showed increased fiber diameter (420 ± 212 nm vs. 284 ± 130 nm). Mechanical properties revealed that the annatto extract induces a reduction of the stiffness of the scaffold. Molecular analyses revealed that while CA scaffold favored C2C12 myoblast differentiation, the annatto-loaded CA scaffold favored a proliferative state of these cells. These results suggest that the combination of cellulose acetate fibers loaded with annatto extract may be an interesting economical alternative for support long-term muscle cells culture with potential application as scaffold for cultivated meat and muscle tissue engineering. |
format | Online Article Text |
id | pubmed-9995891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99958912023-03-10 Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation dos Santos, Ana Elisa Antunes Cotta, Tiago Santos, João Paulo Ferreira Camargos, Juliana Sofia Fonseca do Carmo, Ana Carolina Correia Alcântara, Erika Gabriele Alves Fleck, Claudia Copola, Aline Gonçalves Lio Nogueira, Júlia Meireles Silva, Gerluza Aparecida Borges Andrade, Luciana de Oliveira Ferreira, Roberta Viana Jorge, Erika Cristina Front Bioeng Biotechnol Bioengineering and Biotechnology Electrospinning emerged as a promising technique to produce scaffolds for cultivated meat in function of its simplicity, versatility, cost-effectiveness, and scalability. Cellulose acetate (CA) is a biocompatible and low-cost material that support cell adhesion and proliferation. Here we investigated CA nanofibers, associated or not with a bioactive annatto extract (CA@A), a food-dye, as potential scaffolds for cultivated meat and muscle tissue engineering. The obtained CA nanofibers were evaluated concerning its physicochemical, morphological, mechanical and biological traits. UV-vis spectroscopy and contact angle measurements confirmed the annatto extract incorporation into the CA nanofibers and the surface wettability of both scaffolds, respectively. SEM images revealed that the scaffolds are porous, containing fibers with no specific alignment. Compared with the pure CA nanofibers, CA@A nanofibers showed increased fiber diameter (420 ± 212 nm vs. 284 ± 130 nm). Mechanical properties revealed that the annatto extract induces a reduction of the stiffness of the scaffold. Molecular analyses revealed that while CA scaffold favored C2C12 myoblast differentiation, the annatto-loaded CA scaffold favored a proliferative state of these cells. These results suggest that the combination of cellulose acetate fibers loaded with annatto extract may be an interesting economical alternative for support long-term muscle cells culture with potential application as scaffold for cultivated meat and muscle tissue engineering. Frontiers Media S.A. 2023-02-23 /pmc/articles/PMC9995891/ /pubmed/36911186 http://dx.doi.org/10.3389/fbioe.2023.1116917 Text en Copyright © 2023 Santos, Cotta, Santos, Camargos, Carmo, Alcântara, Fleck, Copola, Nogueira, Silva, Andrade, Ferreira and Jorge. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology dos Santos, Ana Elisa Antunes Cotta, Tiago Santos, João Paulo Ferreira Camargos, Juliana Sofia Fonseca do Carmo, Ana Carolina Correia Alcântara, Erika Gabriele Alves Fleck, Claudia Copola, Aline Gonçalves Lio Nogueira, Júlia Meireles Silva, Gerluza Aparecida Borges Andrade, Luciana de Oliveira Ferreira, Roberta Viana Jorge, Erika Cristina Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
title | Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
title_full | Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
title_fullStr | Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
title_full_unstemmed | Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
title_short | Bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
title_sort | bioactive cellulose acetate nanofiber loaded with annatto support skeletal muscle cell attachment and proliferation |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995891/ https://www.ncbi.nlm.nih.gov/pubmed/36911186 http://dx.doi.org/10.3389/fbioe.2023.1116917 |
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