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Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy
Movement of cellulose synthase particles have so far been observed on the plant epidermis that are amenable to confocal imaging, yielding appreciable signal and resolution to observe small plasma membrane-localised particles. Presented here is a method, using airyscan confocal microscopy, that permi...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996086/ https://www.ncbi.nlm.nih.gov/pubmed/36911339 http://dx.doi.org/10.1016/j.tcsw.2023.100103 |
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author | Wightman, Raymond |
author_facet | Wightman, Raymond |
author_sort | Wightman, Raymond |
collection | PubMed |
description | Movement of cellulose synthase particles have so far been observed on the plant epidermis that are amenable to confocal imaging, yielding appreciable signal and resolution to observe small plasma membrane-localised particles. Presented here is a method, using airyscan confocal microscopy, that permits similar information to be obtained at depth within the developing protoxylem vessels of intact roots. |
format | Online Article Text |
id | pubmed-9996086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-99960862023-03-10 Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy Wightman, Raymond Cell Surf Short Communication Movement of cellulose synthase particles have so far been observed on the plant epidermis that are amenable to confocal imaging, yielding appreciable signal and resolution to observe small plasma membrane-localised particles. Presented here is a method, using airyscan confocal microscopy, that permits similar information to be obtained at depth within the developing protoxylem vessels of intact roots. Elsevier 2023-02-28 /pmc/articles/PMC9996086/ /pubmed/36911339 http://dx.doi.org/10.1016/j.tcsw.2023.100103 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Short Communication Wightman, Raymond Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
title | Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
title_full | Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
title_fullStr | Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
title_full_unstemmed | Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
title_short | Observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
title_sort | observing cellulose synthases at emerging secondary thickenings in developing xylem vessels of the plant root using airyscan confocal microscopy |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996086/ https://www.ncbi.nlm.nih.gov/pubmed/36911339 http://dx.doi.org/10.1016/j.tcsw.2023.100103 |
work_keys_str_mv | AT wightmanraymond observingcellulosesynthasesatemergingsecondarythickeningsindevelopingxylemvesselsoftheplantrootusingairyscanconfocalmicroscopy |