SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus

The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza viru...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xiwen, Li, Song, Qu, Han, Hao, Liangyu, Shao, Ting, Wang, Kai, Xia, Zhiping, Li, Zhiping, Li, Qianxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996428/
https://www.ncbi.nlm.nih.gov/pubmed/36909299
http://dx.doi.org/10.1111/irv.13114
Descripción
Sumario:The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10(−6) TCID(50)/ml. The method showed excellent specificity with no cross‐reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.

Ejemplares similares