SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus

The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza viru...

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Autores principales: Wang, Xiwen, Li, Song, Qu, Han, Hao, Liangyu, Shao, Ting, Wang, Kai, Xia, Zhiping, Li, Zhiping, Li, Qianxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996428/
https://www.ncbi.nlm.nih.gov/pubmed/36909299
http://dx.doi.org/10.1111/irv.13114
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author Wang, Xiwen
Li, Song
Qu, Han
Hao, Liangyu
Shao, Ting
Wang, Kai
Xia, Zhiping
Li, Zhiping
Li, Qianxue
author_facet Wang, Xiwen
Li, Song
Qu, Han
Hao, Liangyu
Shao, Ting
Wang, Kai
Xia, Zhiping
Li, Zhiping
Li, Qianxue
author_sort Wang, Xiwen
collection PubMed
description The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10(−6) TCID(50)/ml. The method showed excellent specificity with no cross‐reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.
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spelling pubmed-99964282023-03-10 SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus Wang, Xiwen Li, Song Qu, Han Hao, Liangyu Shao, Ting Wang, Kai Xia, Zhiping Li, Zhiping Li, Qianxue Influenza Other Respir Viruses Short Communications The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10(−6) TCID(50)/ml. The method showed excellent specificity with no cross‐reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus. John Wiley and Sons Inc. 2023-03-09 /pmc/articles/PMC9996428/ /pubmed/36909299 http://dx.doi.org/10.1111/irv.13114 Text en © 2023 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Wang, Xiwen
Li, Song
Qu, Han
Hao, Liangyu
Shao, Ting
Wang, Kai
Xia, Zhiping
Li, Zhiping
Li, Qianxue
SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
title SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
title_full SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
title_fullStr SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
title_full_unstemmed SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
title_short SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
title_sort sers‐based immunomagnetic bead for rapid detection of h5n1 influenza virus
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996428/
https://www.ncbi.nlm.nih.gov/pubmed/36909299
http://dx.doi.org/10.1111/irv.13114
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