Cargando…
Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats
Periprosthetic joint infection (PJI) is characterized by biofilm infection, which is difficult to alleviate while preserving implant integrity. Furthermore, long-term antibiotic therapy may increase the prevalence of drug-resistant bacterial strains, necessitating a non-antibacterial approach. Adipo...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996572/ https://www.ncbi.nlm.nih.gov/pubmed/36894548 http://dx.doi.org/10.1038/s41598-023-30087-z |
_version_ | 1784903073704968192 |
---|---|
author | Yamamuro, Yuki Kabata, Tamon Nojima, Takayuki Hayashi, Katsuhiro Tokoro, Masaharu Kajino, Yoshitomo Inoue, Daisuke Ohmori, Takaaki Yoshitani, Junya Ueno, Takuro Ueoka, Ken Taninaka, Atsushi Kataoka, Tomoyuki Saiki, Yoshitomo Yanagi, Yu Tsuchiya, Hiroyuki |
author_facet | Yamamuro, Yuki Kabata, Tamon Nojima, Takayuki Hayashi, Katsuhiro Tokoro, Masaharu Kajino, Yoshitomo Inoue, Daisuke Ohmori, Takaaki Yoshitani, Junya Ueno, Takuro Ueoka, Ken Taninaka, Atsushi Kataoka, Tomoyuki Saiki, Yoshitomo Yanagi, Yu Tsuchiya, Hiroyuki |
author_sort | Yamamuro, Yuki |
collection | PubMed |
description | Periprosthetic joint infection (PJI) is characterized by biofilm infection, which is difficult to alleviate while preserving implant integrity. Furthermore, long-term antibiotic therapy may increase the prevalence of drug-resistant bacterial strains, necessitating a non-antibacterial approach. Adipose-derived stem cells (ADSCs) exert antibacterial effects; however, their efficacy in PJI remains unclear. This study investigates the efficacy of combined intravenous ADSCs and antibiotic therapy in comparison to antibiotic monotherapy in a methicillin-sensitive Staphylococcus aureus (MSSA)-infected PJI rat model. The rats were randomly assigned and equally divided into 3 groups: no-treatment group, antibiotic group, ADSCs with antibiotic group. The ADSCs with antibiotic group exhibited the fastest recovery from weight loss, with lower bacterial counts (p = 0.013 vs. no-treatment group; p = 0.024 vs. antibiotic group) and less bone density loss around the implants (p = 0.015 vs. no-treatment group; p = 0.025 vs. antibiotic group). The modified Rissing score was used to evaluate localized infection on postoperative day 14 and was the lowest in the ADSCs with antibiotic group; however, no significant difference was observed between the antibiotic group and ADSCs with antibiotic group (p < 0.001 vs. no-treatment group; p = 0.359 vs. antibiotic group). Histological analysis revealed a clear, thin, and continuous bony envelope, a homogeneous bone marrow, and a defined, normal interface in the ADSCs with antibiotic group. Moreover, the expression of cathelicidin expression was significantly higher (p = 0.002 vs. no-treatment group; p = 0.049 vs. antibiotic group), whereas that of tumor necrosis factor (TNF)-α and interleukin(IL)-6 was lower in the ADSCs with antibiotic group than in the no-treatment group (TNF-α, p = 0.010 vs. no-treatment group; IL-6, p = 0.010 vs. no-treatment group). Thus, the combined intravenous ADSCs and antibiotic therapy induced a stronger antibacterial effect than antibiotic monotherapy in a MSSA-infected PJI rat model. This strong antibacterial effect may be related to the increased cathelicidin expression and decreased inflammatory cytokine expression at the site of infection. |
format | Online Article Text |
id | pubmed-9996572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99965722023-03-09 Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats Yamamuro, Yuki Kabata, Tamon Nojima, Takayuki Hayashi, Katsuhiro Tokoro, Masaharu Kajino, Yoshitomo Inoue, Daisuke Ohmori, Takaaki Yoshitani, Junya Ueno, Takuro Ueoka, Ken Taninaka, Atsushi Kataoka, Tomoyuki Saiki, Yoshitomo Yanagi, Yu Tsuchiya, Hiroyuki Sci Rep Article Periprosthetic joint infection (PJI) is characterized by biofilm infection, which is difficult to alleviate while preserving implant integrity. Furthermore, long-term antibiotic therapy may increase the prevalence of drug-resistant bacterial strains, necessitating a non-antibacterial approach. Adipose-derived stem cells (ADSCs) exert antibacterial effects; however, their efficacy in PJI remains unclear. This study investigates the efficacy of combined intravenous ADSCs and antibiotic therapy in comparison to antibiotic monotherapy in a methicillin-sensitive Staphylococcus aureus (MSSA)-infected PJI rat model. The rats were randomly assigned and equally divided into 3 groups: no-treatment group, antibiotic group, ADSCs with antibiotic group. The ADSCs with antibiotic group exhibited the fastest recovery from weight loss, with lower bacterial counts (p = 0.013 vs. no-treatment group; p = 0.024 vs. antibiotic group) and less bone density loss around the implants (p = 0.015 vs. no-treatment group; p = 0.025 vs. antibiotic group). The modified Rissing score was used to evaluate localized infection on postoperative day 14 and was the lowest in the ADSCs with antibiotic group; however, no significant difference was observed between the antibiotic group and ADSCs with antibiotic group (p < 0.001 vs. no-treatment group; p = 0.359 vs. antibiotic group). Histological analysis revealed a clear, thin, and continuous bony envelope, a homogeneous bone marrow, and a defined, normal interface in the ADSCs with antibiotic group. Moreover, the expression of cathelicidin expression was significantly higher (p = 0.002 vs. no-treatment group; p = 0.049 vs. antibiotic group), whereas that of tumor necrosis factor (TNF)-α and interleukin(IL)-6 was lower in the ADSCs with antibiotic group than in the no-treatment group (TNF-α, p = 0.010 vs. no-treatment group; IL-6, p = 0.010 vs. no-treatment group). Thus, the combined intravenous ADSCs and antibiotic therapy induced a stronger antibacterial effect than antibiotic monotherapy in a MSSA-infected PJI rat model. This strong antibacterial effect may be related to the increased cathelicidin expression and decreased inflammatory cytokine expression at the site of infection. Nature Publishing Group UK 2023-03-09 /pmc/articles/PMC9996572/ /pubmed/36894548 http://dx.doi.org/10.1038/s41598-023-30087-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yamamuro, Yuki Kabata, Tamon Nojima, Takayuki Hayashi, Katsuhiro Tokoro, Masaharu Kajino, Yoshitomo Inoue, Daisuke Ohmori, Takaaki Yoshitani, Junya Ueno, Takuro Ueoka, Ken Taninaka, Atsushi Kataoka, Tomoyuki Saiki, Yoshitomo Yanagi, Yu Tsuchiya, Hiroyuki Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
title | Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
title_full | Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
title_fullStr | Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
title_full_unstemmed | Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
title_short | Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
title_sort | combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996572/ https://www.ncbi.nlm.nih.gov/pubmed/36894548 http://dx.doi.org/10.1038/s41598-023-30087-z |
work_keys_str_mv | AT yamamuroyuki combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT kabatatamon combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT nojimatakayuki combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT hayashikatsuhiro combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT tokoromasaharu combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT kajinoyoshitomo combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT inouedaisuke combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT ohmoritakaaki combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT yoshitanijunya combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT uenotakuro combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT ueokaken combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT taninakaatsushi combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT kataokatomoyuki combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT saikiyoshitomo combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT yanagiyu combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats AT tsuchiyahiroyuki combinedadiposederivedmesenchymalstemcellandantibiotictherapycaneffectivelytreatperiprostheticjointinfectioninrats |