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Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology

OBJECTIVES: Falcataria moluccana, known locally as Sengon, is a fast-growing legume tree that is commonly planted in community forests of Java Island, Indonesia. However, the plantations face attacks of Boktor stem borer (Xystrocera festiva) and gall-rust disease (Uromycladium falcatariae) as major...

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Autores principales: Anita, Vilda Puji Dini, Matra, Deden Derajat, Siregar, Ulfah Juniarti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996948/
https://www.ncbi.nlm.nih.gov/pubmed/36894969
http://dx.doi.org/10.1186/s13104-023-06290-6
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author Anita, Vilda Puji Dini
Matra, Deden Derajat
Siregar, Ulfah Juniarti
author_facet Anita, Vilda Puji Dini
Matra, Deden Derajat
Siregar, Ulfah Juniarti
author_sort Anita, Vilda Puji Dini
collection PubMed
description OBJECTIVES: Falcataria moluccana, known locally as Sengon, is a fast-growing legume tree that is commonly planted in community forests of Java Island, Indonesia. However, the plantations face attacks of Boktor stem borer (Xystrocera festiva) and gall-rust disease (Uromycladium falcatariae) as major threats to its productivity. To control those pest and disease, it is necessary to grow resistant sengon clones, which are developed through tree improvement program, of which needs genetic and genomic information. This dataset was created to construct draft of sengon chloroplast genome and to study the evolution of sengon based on matK and rbcL barcode genes. DATA DESCRIPTION: Genomic DNA was extracted from leaf samples of one individual healthy tree in a private plantation. The DNA was sequenced using Illumina Novaseq 6000 (Novogen AIT, Singapore) for short-reads data, and MinION of Nanopore following manufacture’s protocols SQK-LSK110 for long-reads data. The 66,3 Gb short-reads and 12 Gb long-reads data were hybrid assembled and used to construct a 128.867 bp of F. moluccana chloroplast genome with a quadripartite structure, containing a pair of inverted repeats, a large single-copy and a small single-copy region. Phylogenetic tree constructed using matK and rbcL showed monophyletic origin of F. moluccana and other legume trees.
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spelling pubmed-99969482023-03-10 Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology Anita, Vilda Puji Dini Matra, Deden Derajat Siregar, Ulfah Juniarti BMC Res Notes Research Note OBJECTIVES: Falcataria moluccana, known locally as Sengon, is a fast-growing legume tree that is commonly planted in community forests of Java Island, Indonesia. However, the plantations face attacks of Boktor stem borer (Xystrocera festiva) and gall-rust disease (Uromycladium falcatariae) as major threats to its productivity. To control those pest and disease, it is necessary to grow resistant sengon clones, which are developed through tree improvement program, of which needs genetic and genomic information. This dataset was created to construct draft of sengon chloroplast genome and to study the evolution of sengon based on matK and rbcL barcode genes. DATA DESCRIPTION: Genomic DNA was extracted from leaf samples of one individual healthy tree in a private plantation. The DNA was sequenced using Illumina Novaseq 6000 (Novogen AIT, Singapore) for short-reads data, and MinION of Nanopore following manufacture’s protocols SQK-LSK110 for long-reads data. The 66,3 Gb short-reads and 12 Gb long-reads data were hybrid assembled and used to construct a 128.867 bp of F. moluccana chloroplast genome with a quadripartite structure, containing a pair of inverted repeats, a large single-copy and a small single-copy region. Phylogenetic tree constructed using matK and rbcL showed monophyletic origin of F. moluccana and other legume trees. BioMed Central 2023-03-09 /pmc/articles/PMC9996948/ /pubmed/36894969 http://dx.doi.org/10.1186/s13104-023-06290-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Anita, Vilda Puji Dini
Matra, Deden Derajat
Siregar, Ulfah Juniarti
Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
title Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
title_full Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
title_fullStr Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
title_full_unstemmed Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
title_short Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
title_sort chloroplast genome draft assembly of falcataria moluccana using hybrid sequencing technology
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9996948/
https://www.ncbi.nlm.nih.gov/pubmed/36894969
http://dx.doi.org/10.1186/s13104-023-06290-6
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