Standardization of flow cytometry and cell sorting to enable a transcriptomic analysis in a multi-site sarcoidosis study

The contribution and regulation of various CD4(+) T cell lineages that occur with remitting vs progressive courses in sarcoidosis are poorly understood. We developed a multiparameter flow cytometry panel to sort these CD4(+) T cell lineages followed by measurement of their functional potential using...

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Detalles Bibliográficos
Autores principales: Magallon, Roman E., Harmacek, Laura D., Arger, Nicholas K., Grewal, Pineet, Powers, Linda, Werner, Brenda R., Barkes, Briana Q., Li, Li, MacPhail, Kristyn, Gillespie, May, White, Elizabeth K., Collins, Sarah E., Brown, Talyor, Cardenas, Jessica, Chen, Edward S., Maier, Lisa A., Leach, Sonia M., Hamzeh, Nabeel Y., Koth, Laura L., O’Connor, Brian P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9997938/
https://www.ncbi.nlm.nih.gov/pubmed/36893197
http://dx.doi.org/10.1371/journal.pone.0281210

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