Microbacterium elymi sp. nov., Isolated from the Rhizospheric Soil of Elymus tsukushiensis, a Plant Native to the Dokdo Islands, Republic of Korea

Microbacterium elymi KUDC0405(T) was isolated from the rhizosphere of Elymus tsukushiensis from the Dokdo Islands. The KUDC0405(T) strain was Gram-stain-positive, non-spore forming, non-motile, and facultatively anaerobic bacteria. Strain KUDC0405(T) was a rod-shaped bacterium with size dimensions of 0.3–0.4 × 0.7–0.8 μm. Based on 16S rRNA gene sequences, KUDC0405(T) was most closely related to Microbacterium bovistercoris NEAU-LLE(T) (97.8%) and Microbacterium pseudoresistens CC-5209(T) (97.6%). The dDDH (digital DNA-DNA hybridization) values between KUDC0405(T) and M. bovistercoris NEAU-LLE(T) and M. pseudoresistens CC-5209(T) were below 17.3% and 17.5%, respectively. The ANI (average nucleotide identity) values among strains KUDC0405(T), M. bovistercoris NEAU-LLE(T), and M. pseudoresistens CC-5209(T) were 86.6% and 80.7%, respectively. The AAI (average amino acid identity) values were 64.66% and 64.97%, respectively, between KUDC0405(T) and its closest related type strains. The genome contained 3,596 CDCs, three rRNAs, 46 tRNAs, and three non-coding RNAs (ncRNAs). The genomic DNA GC content was 70.4%. The polar lipids included diphosphatydilglycerol, glycolipid, phosphatydilglycerol, and unknown phospholipid, and the major fatty acids were anteiso-C(17:0) and iso-C(16:0). Strain KUDC0405(T) contained MK-12 as the major menaquinone. Based on genotypic, phylogenetic, and phenotypic properties, strain KUDC0405(T) should be considered a novel species within the genus Microbacterium, for which we propose the name M. elymi sp. nov., and the type strain as KUDC0405(T) (=KCTC 49411(T), =CGMCC1.18472(T)).