Cargando…

Biological synthesis of ursodeoxycholic acid

Ursodeoxycholic acid (UDCA) is a fundamental treatment drug for numerous hepatobiliary diseases that also has adjuvant therapeutic effects on certain cancers and neurological diseases. Chemical UDCA synthesis is environmentally unfriendly with low yields. Biological UDCA synthesis by free-enzyme cat...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Peng, Zhang, Xue, Feng, Wei, Xu, Wei, Wu, Chaoyun, Xie, Shaoqing, Yu, Sisi, Fu, Rongzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9998936/
https://www.ncbi.nlm.nih.gov/pubmed/36910199
http://dx.doi.org/10.3389/fmicb.2023.1140662
_version_ 1784903561070510080
author Song, Peng
Zhang, Xue
Feng, Wei
Xu, Wei
Wu, Chaoyun
Xie, Shaoqing
Yu, Sisi
Fu, Rongzhao
author_facet Song, Peng
Zhang, Xue
Feng, Wei
Xu, Wei
Wu, Chaoyun
Xie, Shaoqing
Yu, Sisi
Fu, Rongzhao
author_sort Song, Peng
collection PubMed
description Ursodeoxycholic acid (UDCA) is a fundamental treatment drug for numerous hepatobiliary diseases that also has adjuvant therapeutic effects on certain cancers and neurological diseases. Chemical UDCA synthesis is environmentally unfriendly with low yields. Biological UDCA synthesis by free-enzyme catalysis or whole-cell synthesis using inexpensive and readily available chenodeoxycholic acid (CDCA), cholic acid (CA), or lithocholic acid (LCA) as substrates is being developed. The free enzyme-catalyzed one-pot, one-step/two-step method uses hydroxysteroid dehydrogenase (HSDH); whole-cell synthesis, mainly uses engineered bacteria (mainly Escherichia coli) expressing the relevant HSDHs. To further develop these methods, HSDHs with specific coenzyme dependence, high enzyme activity, good stability, and high substrate loading concentration, P450 monooxygenase with C-7 hydroxylation activity and engineered strain harboring HSDHs must be exploited.
format Online
Article
Text
id pubmed-9998936
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99989362023-03-11 Biological synthesis of ursodeoxycholic acid Song, Peng Zhang, Xue Feng, Wei Xu, Wei Wu, Chaoyun Xie, Shaoqing Yu, Sisi Fu, Rongzhao Front Microbiol Microbiology Ursodeoxycholic acid (UDCA) is a fundamental treatment drug for numerous hepatobiliary diseases that also has adjuvant therapeutic effects on certain cancers and neurological diseases. Chemical UDCA synthesis is environmentally unfriendly with low yields. Biological UDCA synthesis by free-enzyme catalysis or whole-cell synthesis using inexpensive and readily available chenodeoxycholic acid (CDCA), cholic acid (CA), or lithocholic acid (LCA) as substrates is being developed. The free enzyme-catalyzed one-pot, one-step/two-step method uses hydroxysteroid dehydrogenase (HSDH); whole-cell synthesis, mainly uses engineered bacteria (mainly Escherichia coli) expressing the relevant HSDHs. To further develop these methods, HSDHs with specific coenzyme dependence, high enzyme activity, good stability, and high substrate loading concentration, P450 monooxygenase with C-7 hydroxylation activity and engineered strain harboring HSDHs must be exploited. Frontiers Media S.A. 2023-02-24 /pmc/articles/PMC9998936/ /pubmed/36910199 http://dx.doi.org/10.3389/fmicb.2023.1140662 Text en Copyright © 2023 Song, Zhang, Feng, Xu, Wu, Xie, Yu and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Song, Peng
Zhang, Xue
Feng, Wei
Xu, Wei
Wu, Chaoyun
Xie, Shaoqing
Yu, Sisi
Fu, Rongzhao
Biological synthesis of ursodeoxycholic acid
title Biological synthesis of ursodeoxycholic acid
title_full Biological synthesis of ursodeoxycholic acid
title_fullStr Biological synthesis of ursodeoxycholic acid
title_full_unstemmed Biological synthesis of ursodeoxycholic acid
title_short Biological synthesis of ursodeoxycholic acid
title_sort biological synthesis of ursodeoxycholic acid
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9998936/
https://www.ncbi.nlm.nih.gov/pubmed/36910199
http://dx.doi.org/10.3389/fmicb.2023.1140662
work_keys_str_mv AT songpeng biologicalsynthesisofursodeoxycholicacid
AT zhangxue biologicalsynthesisofursodeoxycholicacid
AT fengwei biologicalsynthesisofursodeoxycholicacid
AT xuwei biologicalsynthesisofursodeoxycholicacid
AT wuchaoyun biologicalsynthesisofursodeoxycholicacid
AT xieshaoqing biologicalsynthesisofursodeoxycholicacid
AT yusisi biologicalsynthesisofursodeoxycholicacid
AT furongzhao biologicalsynthesisofursodeoxycholicacid