Cargando…
Biological synthesis of ursodeoxycholic acid
Ursodeoxycholic acid (UDCA) is a fundamental treatment drug for numerous hepatobiliary diseases that also has adjuvant therapeutic effects on certain cancers and neurological diseases. Chemical UDCA synthesis is environmentally unfriendly with low yields. Biological UDCA synthesis by free-enzyme cat...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9998936/ https://www.ncbi.nlm.nih.gov/pubmed/36910199 http://dx.doi.org/10.3389/fmicb.2023.1140662 |
_version_ | 1784903561070510080 |
---|---|
author | Song, Peng Zhang, Xue Feng, Wei Xu, Wei Wu, Chaoyun Xie, Shaoqing Yu, Sisi Fu, Rongzhao |
author_facet | Song, Peng Zhang, Xue Feng, Wei Xu, Wei Wu, Chaoyun Xie, Shaoqing Yu, Sisi Fu, Rongzhao |
author_sort | Song, Peng |
collection | PubMed |
description | Ursodeoxycholic acid (UDCA) is a fundamental treatment drug for numerous hepatobiliary diseases that also has adjuvant therapeutic effects on certain cancers and neurological diseases. Chemical UDCA synthesis is environmentally unfriendly with low yields. Biological UDCA synthesis by free-enzyme catalysis or whole-cell synthesis using inexpensive and readily available chenodeoxycholic acid (CDCA), cholic acid (CA), or lithocholic acid (LCA) as substrates is being developed. The free enzyme-catalyzed one-pot, one-step/two-step method uses hydroxysteroid dehydrogenase (HSDH); whole-cell synthesis, mainly uses engineered bacteria (mainly Escherichia coli) expressing the relevant HSDHs. To further develop these methods, HSDHs with specific coenzyme dependence, high enzyme activity, good stability, and high substrate loading concentration, P450 monooxygenase with C-7 hydroxylation activity and engineered strain harboring HSDHs must be exploited. |
format | Online Article Text |
id | pubmed-9998936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99989362023-03-11 Biological synthesis of ursodeoxycholic acid Song, Peng Zhang, Xue Feng, Wei Xu, Wei Wu, Chaoyun Xie, Shaoqing Yu, Sisi Fu, Rongzhao Front Microbiol Microbiology Ursodeoxycholic acid (UDCA) is a fundamental treatment drug for numerous hepatobiliary diseases that also has adjuvant therapeutic effects on certain cancers and neurological diseases. Chemical UDCA synthesis is environmentally unfriendly with low yields. Biological UDCA synthesis by free-enzyme catalysis or whole-cell synthesis using inexpensive and readily available chenodeoxycholic acid (CDCA), cholic acid (CA), or lithocholic acid (LCA) as substrates is being developed. The free enzyme-catalyzed one-pot, one-step/two-step method uses hydroxysteroid dehydrogenase (HSDH); whole-cell synthesis, mainly uses engineered bacteria (mainly Escherichia coli) expressing the relevant HSDHs. To further develop these methods, HSDHs with specific coenzyme dependence, high enzyme activity, good stability, and high substrate loading concentration, P450 monooxygenase with C-7 hydroxylation activity and engineered strain harboring HSDHs must be exploited. Frontiers Media S.A. 2023-02-24 /pmc/articles/PMC9998936/ /pubmed/36910199 http://dx.doi.org/10.3389/fmicb.2023.1140662 Text en Copyright © 2023 Song, Zhang, Feng, Xu, Wu, Xie, Yu and Fu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Song, Peng Zhang, Xue Feng, Wei Xu, Wei Wu, Chaoyun Xie, Shaoqing Yu, Sisi Fu, Rongzhao Biological synthesis of ursodeoxycholic acid |
title | Biological synthesis of ursodeoxycholic acid |
title_full | Biological synthesis of ursodeoxycholic acid |
title_fullStr | Biological synthesis of ursodeoxycholic acid |
title_full_unstemmed | Biological synthesis of ursodeoxycholic acid |
title_short | Biological synthesis of ursodeoxycholic acid |
title_sort | biological synthesis of ursodeoxycholic acid |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9998936/ https://www.ncbi.nlm.nih.gov/pubmed/36910199 http://dx.doi.org/10.3389/fmicb.2023.1140662 |
work_keys_str_mv | AT songpeng biologicalsynthesisofursodeoxycholicacid AT zhangxue biologicalsynthesisofursodeoxycholicacid AT fengwei biologicalsynthesisofursodeoxycholicacid AT xuwei biologicalsynthesisofursodeoxycholicacid AT wuchaoyun biologicalsynthesisofursodeoxycholicacid AT xieshaoqing biologicalsynthesisofursodeoxycholicacid AT yusisi biologicalsynthesisofursodeoxycholicacid AT furongzhao biologicalsynthesisofursodeoxycholicacid |