-
1por Gallagher, Kelley A., Schumacher, Maria A., Bush, Matthew J., Bibb, Maureen J., Chandra, Govind, Holmes, Neil A., Zeng, Wenjie, Henderson, Max, Zhang, Hengshan, Findlay, Kim C., Brennan, Richard G., Buttner, Mark J.“…Here we demonstrate that c-di-GMP also intervenes later in development to control differentiation of the reproductive hyphae into spores by arming a novel anti-σ (RsiG) to bind and sequester a sporulation-specific σ factor (σ(WhiG)). We present the structure of the RsiG-(c-di-GMP)(2)-σ(WhiG) complex, revealing an unusual, partially intercalated c-di-GMP dimer bound at the RsiG-σ(WhiG) interface. …”
Publicado 2020
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
2por Schumacher, Maria A., Gallagher, Kelley A., Holmes, Neil A., Chandra, Govind, Henderson, Max, Kysela, David T., Brennan, Richard G., Buttner, Mark J.“…We recently showed c-di-GMP controls this transition by arming a unique anti-σ, RsiG, to bind the sporulation-specific σ, WhiG. The Streptomyces venezuelae RsiG–(c-di-GMP)(2)–WhiG structure revealed that a monomeric RsiG binds c-di-GMP via two E(X)(3)S(X)(2)R(X)(3)Q(X)(3)D repeat motifs, one on each helix of an antiparallel coiled-coil. …”
Publicado 2021
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto -
3por Schumacher, Maria A., Wörmann, Mirka E., Henderson, Max, Salinas, Raul, Latoscha, Andreas, Al-Bassam, Mahmoud M., Singh, Kumar Siddharth, Barclay, Elaine, Gunka, Katrin, Tschowri, Natalia“…Streptomyces are our principal source of antibiotics, which they generate concomitant with a complex developmental transition from vegetative hyphae to spores. c-di-GMP acts as a linchpin in this transition by binding and regulating the key developmental regulators, BldD and WhiG. Here we show that c-di-GMP also binds the glycogen-debranching-enzyme, GlgX, uncovering a direct link between c-di-GMP and glycogen metabolism in bacteria. …”
Publicado 2022
Enlace del recurso
Enlace del recurso
Enlace del recurso
Online Artículo Texto