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1por Masumoto, Hidetoshi, Ikuno, Takeshi, Takeda, Masafumi, Fukushima, Hiroyuki, Marui, Akira, Katayama, Shiori, Shimizu, Tatsuya, Ikeda, Tadashi, Okano, Teruo, Sakata, Ryuzo, Yamashita, Jun K.“…Here we report a new protocol for the simultaneous induction of cardiomyocytes (CMs) and vascular cells [endothelial cells (ECs)/vascular mural cells (MCs)], and generate entirely hiPSC-engineered cardiovascular cell sheets, which showed advantageous therapeutic effects in infarcted hearts. …”
Publicado 2014
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2por Matsuo, Takehiko, Masumoto, Hidetoshi, Tajima, Shuhei, Ikuno, Takeshi, Katayama, Shiori, Minakata, Kenji, Ikeda, Tadashi, Yamamizu, Kohei, Tabata, Yasuhiko, Sakata, Ryuzo, Yamashita, Jun K.“…We previously generated cardiovascular cell sheets entirely from pluripotent stem cells with cardiomyocytes, endothelial cells and vascular mural cells. Though sheet transplantation showed a better engraftment and improved cardiac function after myocardial infarction, stacking limitation (up to 3 sheets) by hypoxia hampered larger structure formation and long-term survival of the grafts. …”
Publicado 2015
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3por Masumoto, Hidetoshi, Nakane, Takeichiro, Tinney, Joseph P., Yuan, Fangping, Ye, Fei, Kowalski, William J., Minakata, Kenji, Sakata, Ryuzo, Yamashita, Jun K., Keller, Bradley B.“…We generated engineered cardiac tissues (ECTs) from three cellular compositions of cardiomyocytes (CMs), endothelial cells (ECs), and vascular mural cells (MCs) differentiated from hiPSCs. We then determined the impact of cell composition on ECT structural and functional properties. …”
Publicado 2016
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4por Nakane, Takeichiro, Masumoto, Hidetoshi, Tinney, Joseph P., Yuan, Fangping, Kowalski, William J., Ye, Fei, LeBlanc, Amanda J., Sakata, Ryuzo, Yamashita, Jun K., Keller, Bradley B.“…We explored multiple 15 × 15 mm ECT geometries using molds with rectangular internal staggered posts (mesh, ME), without posts (plain sheet, PS), or long parallel posts (multiple linear bundles, ML) and a gel matrix containing hiPSC-derived cardiomyocytes, endothelial, and vascular mural cells matured in vitro for 14 days. ME-ECTs displayed the lowest dead cell ratio (p < 0.001) and matured into 0.5 mm diameter myofiber bundles with greater 3D cell alignment and higher active stress than PS-ECTs. …”
Publicado 2017
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5por Ishigami, Masanosuke, Masumoto, Hidetoshi, Ikuno, Takeshi, Aoki, Takayuki, Kawatou, Masahide, Minakata, Kenji, Ikeda, Tadashi, Sakata, Ryuzo, Yamashita, Jun K., Minatoya, Kenji“…We simultaneously induced cardiomyocytes (CMs) and vascular cells [vascular endothelial cells (ECs) and vascular mural cells (MCs)] from hiPSCs. We generated L-CTSs using 10cm-sized temperature-responsive culture dishes. …”
Publicado 2018
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