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  1. 1
    “…With the assistance of computational microscopy, CHAMP enables high‐throughput and label‐free imaging of thick and unprocessed tissues with large surface irregularity at an acquisition speed of 10 mm(2)/10 s with 1.1‐µm lateral resolution. …”
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    High‐Throughput, Label‐Free and Slide‐Free Histological Imaging by Computational Microscopy and Unsupervised Learning
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    “…Present results can resolve mineral exhaustion issues in the development of next-generation hard magnetic materials because the alloys are free from rare-earth elements, and the technique is well suited for mass production.…”
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    Artificially produced rare-earth free cosmic magnet
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    “…Epigenetic alterations, including 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and nucleosome positioning (NP), in cell-free DNA (cfDNA) have been widely observed in human diseases, and many available cfDNA-based epigenome-wide profiles exhibit high sensitivity and specificity in disease detection and classification. …”
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    CFEA: a cell-free epigenome atlas in human diseases
  4. 4
    por Zhang, Yan, Nezis, Ioannis P.
    Publicado 2022
    “…In this protocol, we describe steps to identify Drosophila LIRCPs, in Atg8a LDS mutants we have created, via label-free quantitative proteomic analysis. We detail steps for extraction of proteins from adult Drosophila heads, followed by liquid chromatography–mass spectrometry (LC-MS/MS) analysis. …”
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    Label-free quantitative proteomic analysis of adult Drosophila heads
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    ElectroPen: An ultra-low–cost, electricity-free, portable electroporator
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    “…BACKGROUND: The prenatal test of cell-free fetal DNA (cffDNA) is also known as noninvasive prenatal testing (NIPT) with high sensitivity and specificity. …”
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    Cell-free fetal DNA testing and its correlation with prenatal indications
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    Exploration of Major Cognitive Deficits in Medication-Free Patients With Major Depressive Disorder
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    “…We used these MRPs to examine Met oxidation and MetO reduction by in-gel shift assays and immunoblot assays with antibodies generated against various oxidized MRPs. The oxidation of Cys-free MRPs by hydrogen peroxide could be conveniently monitored by SDS-PAGE and was specific for Met, as evidenced by quantitative reduction of these proteins with Msrs in DTT- and thioredoxin-dependent assays. …”
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    Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins
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    “…Here we explored the diagnostic potential of 5hmC in circulating cell-free DNA (cfDNA) using a sensitive chemical labeling-based low-input shotgun sequencing approach. …”
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    5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages
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    “…In the present study, the in vitro and cellular free radical scavenging activities of corn peptide fractions (CPFs) were investigated. …”
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    Isolation and Characterisation of in Vitro and Cellular Free Radical Scavenging Peptides from Corn Peptide Fractions
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    “…MSCs-derived extracellular vesicles (MSCs-EV) is indicated as a novel cell-free alternative to cell therapy with MSCs in regenerative medicine. …”
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    The Application of MSCs-Derived Extracellular Vesicles in Bone Disorders: Novel Cell-Free Therapeutic Strategy
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    “…Here we propose a label-free, cost-effective, and ready-to-use 3D histological imaging technique, termed microtomy-assisted autofluorescence tomography with ultraviolet excitation (MATE). …”
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    Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography
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    The Free Zinc Concentration in the Synaptic Cleft of Artificial Glycinergic Synapses Rises to At least 1 μM
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    “…OBJECTIVES: The purpose of this study was to segment the left ventricle (LV) blood pool, LV myocardium, and right ventricle (RV) blood pool of end-diastole and end-systole frames in free-breathing cardiac magnetic resonance (CMR) imaging. …”
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    A Deep Learning Segmentation Approach in Free-Breathing Real-Time Cardiac Magnetic Resonance Imaging
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    “…Herein, the development of a cell‐free protein synthesis (CFPS) platform that enables rapid genome mining, screening, and guided overproduction of lanthipeptides in vivo is described. …”
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    A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo
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    “…In present work, the abrasive-free jet polishing (AFJP) of bulk single-crystal KDP was first fulfilled, when using a newly-designed low-viscosity microemulsion as the AFJP fluid. …”
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    Novel abrasive-free jet polishing for Bulk single-crystal KDP with a low viscosity microemulsion
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    por Xiao, Sa, Zhang, Yan
    Publicado 2020
    “…METHODS: Adult mouse LGSCs were cultured in Matrigel-based 3D culture under serum-free culture condition, which contained EGF, FGF10, Wnt3A, and Y-27632. …”
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    Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair
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    Template-Free Synthesis of N-Doped Porous Carbon Materials From Furfuryl Amine-Based Protic Salts
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    Human Amniotic Fluid Stem Cell-Derived Exosomes as a Novel Cell-Free Therapy for Cutaneous Regeneration
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    “…As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). …”
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    A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
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