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421por Stewart, Cameron R., Deffrasnes, Celine, Foo, Chwan Hong, Bean, Andrew G. D., Wang, Lin-Fa“…Hendra and Nipah viruses (family Paramyxoviridae, genus Henipavirus) are zoonotic RNA viruses that cause lethal disease in humans and are designated as Biosafety Level 4 (BSL4) agents. Moreover, henipaviruses belong to the same group of viruses that cause disease more commonly in humans such as measles, mumps and respiratory syncytial virus. …”
Publicado 2017
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422por Biedunkiewicz, Anna, Sucharzewska, Ewa, Kulesza, Kamila, Nowacka, Karolina, Kubiak, Dariusz“…Nine species were categorised as class BSL-2 fungi. This study provides a valuable complement of data concerning the natural composition of the littoral microbiota.…”
Publicado 2019
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423“…HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. …”
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424por Földes, Fanni, Madai, Mónika, Papp, Henrietta, Kemenesi, Gábor, Zana, Brigitta, Geiger, Lili, Gombos, Katalin, Somogyi, Balázs, Bock-Marquette, Ildikó, Jakab, Ferenc“…The highly pathogenic characteristic of CCHFV restricts research to BSL-4 laboratories, which complicates effective research and developmental strategies. …”
Publicado 2020
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425por Hartlaub, Julia, von Arnim, Felicitas, Fast, Christine, Mirazimi, Ali, Keller, Markus, Groschup, Martin H.“…Humans can suffer from a mild febrile illness, hence, DUGV is classified as a biosafety level (BSL) 3 agent. In contrast, no disease has been described in animals, albeit serological evidence exists that ruminants are common hosts and may play an important role in the transmission cycle of this neglected arbovirus. …”
Publicado 2021
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426por Mishra, Tarun, Sreepadmanabh, M., Ramdas, Pavitra, Sahu, Amit Kumar, Kumar, Atul, Chande, Ajit“…The establishment of SARS CoV-2 spike-pseudotyped lentiviral (LV) systems has enabled the rapid identification of entry inhibitors and neutralizing agents, alongside allowing for the study of this emerging pathogen in BSL-2 level facilities. While such frameworks recapitulate the cellular entry process in ACE2+ cells, they are largely unable to factor in supplemental contributions by other SARS CoV-2 genes. …”
Publicado 2021
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427“…In addition, the piglets raised in BSL3 conditions showed an increased richness of low-abundant species in the nasal microbiota. …”
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428por Cao, Xiaofang, Sandberg, AnnSofi, Araújo, José Eduardo, Cvetkovski, Filip, Berglund, Erik, Eriksson, Lars E., Pernemalm, Maria“…In addition, we evaluate the effect of viral heat inactivation on the plasma proteome, an additional step in the plasma preparation workflow that allows the sample preparation of SARS-Cov2-infected samples to be performed in a BSL3 laboratory, and report the advantage of performing the heat inactivation postdepletion. …”
Publicado 2021
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429por Trujillo, Monica, Cheung, Kristen, Gao, Anna, Hoxie, Irene, Kannoly, Sherin, Kubota, Nanami, San, Kaung Myat, Smyth, Davida S., Dennehy, John J.“…Owing to a pasteurization step, it is safe for use in a BSL2 facility. In addition to making the protocol safe for the personnel involved, pasteurization had the added benefit of increasing the SARS-CoV-2 genetic signal. …”
Publicado 2021
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430“…Using these protocols, a BSL-2 laboratory equipped for cell culture work would generate at least 6.0 log plaque-forming units (PFU) per mL of MHV lysate and provide an optimized overlay assay using either methylcellulose or agarose as overlays for the titration of infectious virus particles. …”
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431por Behnia, Mahgol, Baer, Alan, Skidmore, Andrew M., Lehman, Caitlin W., Bracci, Nicole, Kehn-Hall, Kylene, Bradfute, Steven B.“…Therefore, RNA isolated from cells infected with BSL-3 select agent strains of VEEV or other positive-strand viruses must be inactivated before removal from high-containment laboratories. …”
Publicado 2022
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432por Lowman, Kelsey L., Patrick, Christopher J., Perkins, Emily R., Bottesi, Gioia, Caruso, Maria, Giulini, Paolo, Sica, ClaudioEnlace del recurso
Publicado 2021
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435por Poelzl, Sabine, Rieger, Julia, Zatloukal, Kurt, Augl, Stefan, Stummer, Maximilian, Hinterer, Andreas, Kittinger, Clemens“…Since tests with in vitro-propagated SARS-CoV-2 require biosafety level 3 (BSL-3) laboratories with limited capacities and high costs, experiments with an appropriate surrogate like the bacteriophage ɸ6 are preferred in most studies. …”
Publicado 2023
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436por Hsu, Chi-Ju, Chen, Cheng-Hsiu, Chen, Wen-Ting, Liu, Ping-Cheng, Chang, Tein-Yao, Lin, Meng-He, Chen, Cheng-Cheung, Chen, Hsing-Yu, Huang, Chih-Heng, Cheng, Yun-Hsiang, Sun, Jun-Ren“…The Ebola virus minigenome system enables researchers to study the Ebola virus under BSL-2 conditions. Here, we found that the addition of the nucleocapsid protein of human coronaviruses, such as severe acute respiratory syndrome coronavirus 2, can increase the ratio of green fluorescent protein-positive cells by 1.5–2 folds in the Ebola virus minigenome system. …”
Publicado 2023
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437por Welsch, Sonja, Kolesnikova, Larissa, Krähling, Verena, Riches, James D., Becker, Stephan, Briggs, John A. G.“…Here we have infected cells with Marburg virus under BSL-4 containment conditions, and reconstructed the sequence of steps in the budding process in three dimensions using electron tomography of plastic-embedded cells. …”
Publicado 2010
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438por Rose, Helen L., Dewey, Caroline A., Ely, Morgan S., Willoughby, Sarah L., Parsons, Tanya M., Cox, Victoria, Spencer, Phillippa M., Weller, Simon A.“…Eight DNA extraction products or methods (Applied Biosystems PrepFiler Forensic DNA Extraction Kit; Bio-Rad Instagene Only, Bio-Rad Instagene & Spin Column Purification; EpiCentre MasterPure DNA & RNA Kit; FujiFilm QuickGene Mini80; Idaho Technologies 1-2-3 Q-Flow Kit; MoBio UltraClean Microbial DNA Isolation Kit; Sigma Extract-N-Amp Plant and Seed Kit) were adapted to facilitate extraction of DNA under BSL3 containment conditions. DNA was extracted from 12 common interferents or sample types, spiked with spores of Bacillus atropheaus. …”
Publicado 2011
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439por Waters, Dafydd, Campbell, Ruth, Capek, Cheryl M., Woll, Bencie, David, Anthony S., McGuire, Philip K., Brammer, Michael J., MacSweeney, Mairéad“…Using fMRI, we compared cortical networks supporting the perception of fingerspelled, signed, written, and pictorial stimuli in deaf native signers of British Sign Language (BSL). In order to examine the effects of linguistic knowledge, hearing participants who knew neither fingerspelling nor a signed language were also tested. …”
Publicado 2007
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440por IBRAHIM, Dalia, NAKAMUTA, Nobuaki, TANIGUCHI, Kazumi, YAMAMOTO, Yoshio, TANIGUCHI, Kazuyuki“…The free border of both of the olfactory and respiratory epithelia was stained with 12 lectins: Wheat germ agglutinin (WGA), succinylated-wheat germ agglutinin (s-WGA), Lycopersicon esculentum lectin (LEL), Solanum tuberosum lectin (STL), Datura stramonium lectin (DSL), Soybean agglutinin (SBA), Bandeiraea simplicifolia lectin-I (BSL-I), Ricinus communis agglutinin-I (RCA-120), Erythrina cristagalli lectin (ECL), Concanavalin A (Con A), Phaseolus vulgaris agglutinin-E (PHA-E) and Phaseolus vulgaris agglutinin-L (PHA-L). …”
Publicado 2013
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