“…DM was also used to compare the OTUs from the T-RFLP data with seven restriction enzymes (two enzymes of 516f-BslI and 516f-HaeIII, two enzymes of 27f-MspI and 27f-AluI, three enzymes of 35f-HhaI, 35f-MspI and 35f-AluI) and their various combinations. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Development of facilities for diagnosis of virus from insects, reservoirs, and human beings (like BSL4, which has been established in NIV, Pune), awareness about symptoms of new emerging viral and other zoonotic diseases, differential diagnosis, risk factors (climatic, ecological, and socioeconomic) and mapping of disease-specific vulnerable areas, and mathematical modeling for projecting epidemiological scenario is needed for preparedness of public health institutes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Integrity of this CRE, designated SL9266 (alternatively 5BSL3.2), is critical for genome replication. SL9266 forms the core of an extended pseudoknot, designated SL9266/PK, involving long distance RNA–RNA interactions between unpaired loops of SL9266 and distal regions of the genome. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities. The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…A plaque assay for quantitating filoviruses in virus stocks, prepared viral challenge inocula and samples from research animals has recently been fully characterized and standardized for use across multiple institutions performing Biosafety Level 4 (BSL-4) studies. After standardization studies were completed, Good Laboratory Practices (GLP)-compliant plaque assay method validation studies to demonstrate suitability for reliable and reproducible measurement of the Marburg Virus Angola (MARV) variant and Ebola Virus Kikwit (EBOV) variant commenced at the United States Army Medical Research Institute of Infectious Diseases (USAMRIID). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The vast experience from the existing studies at Plum Island Animal Disease Center, other U.S. laboratories, and those in Europe and Australia with biosafety level 4 (BSL-4) facilities designed for large animals, clearly demonstrates the valuable contribution this capability brings to the efforts to detect, prepare, prevent and respond to livestock and potential zoonotic threats. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These studies are important for preventing the spread and outbreak of zoonotic viruses like RVFV and are supported by the Canadian-led BSL4ZNet network.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Experiments conducted on our own database but also on four benchmark databases (Stirling’s, Alicante, Essex, and Stegmann’s) and on deaf individuals from normal 2D videos are freely available on the BSL signbank dataset. Results demonstrate the validity of our system to solve problems of face and hand segmentation and reconstruction under different environmental conditions.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The observed differences are most likely the result of the outbred nature of macaques and low animal numbers often used by necessity and for ethical considerations in BSL-4 studies. Nonetheless, while we were unable to achieve severe disease or lethality, the CCHFV Hoti and Afghanistan macaque models are useful for screening medical countermeasures using biomarkers including viremia and clinical pathology to assess efficacy.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Product development and manufacturing process were carried out in a BSL-3 facility. Immunogenicity and safety were determined at two antigen concentrations (3μg and 6μg), with two different adjuvants, in mice, rats, and rabbits. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The risk posed by Severe Acute Respiratory Syndrome Coronavirus -2 (SARS-CoV-2) dictates that live-virus research is conducted in a biosafety level 3 (BSL3) facility. Working with SARS-CoV-2 at lower biosafety levels can expedite research yet requires the virus to be fully inactivated. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The recordings were obtained in a digital acquisition system (BSL 4.0 MP 36, Biopac Systems, Inc., USA). RESULTS: In the present investigation, ibotenic acid lesion of mediodorsal thalamic nuclei reduced the wakefulness and increased paradoxical sleep, which contradicts the reports from earlier lesion studies in cats. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Given the broad-spectrum antimicrobial activity of polyphenols and the urgency to develop therapies for the treatment of CCHF, we investigated the antiviral activity of cranberry extract against HAZV, a surrogate nairovirus model of CCHFV that can be handled in Level 2 Biosafety Laboratories (BSL-2). The results indicate that the cranberry extract exerts an antiviral activity against HAZV by targeting early stages of the viral replication cycle, including the initial adsorption to target cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We developed the bsr1-NIL with resistance to BS and applied it for variety registration to Ministry of Agriculture, Forestry and Fisheries in Japan as ‘Mienoyume BSL’. This is the first report for the BS resistant rice variety bred using marker-assisted selection.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Our work established a practical pseudovirus-based neutralization assay for SARS-CoV-2 variants, which can be conducted safely under biosafety level-2 (BSL-2) conditions, and this assay will be a promising tool for studying and characterizing vaccines and therapeutic candidates against Omicron-included SARS-CoV-2 variants.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The SARS-CoV-2 pandemic and the urgent need for massive antiviral testing highlighted the lack of a good cell-based assay that allowed for a fast, automated screening of antivirals in high-throughput content with minimal handling requirements in a BSL-3 environment. The present paper describes the construction of a green fluorescent substrate that, upon cleavage by the SARS-CoV-2 main protease, re-localizes from the cytoplasm in non-infected cells to the nucleus in infected cells. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To address this issue, we used sequential infections with well-defined BSL-2 pathogens to establish a novel immune-experienced mouse model (specific pathogen experienced [SPexp]) to determine the extent to which immunological experience and/or inflammation influences the host capacity to respond to subsequent infections, including sepsis. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Crimean-Congo hemorrhagic fever virus (CCHFV) is a biosafety level-4 (BSL-4) pathogen that causes Crimean-Congo hemorrhagic fever (CCHF) characterized by hemorrhagic manifestation, multiple organ failure and high mortality rate, posing great threat to public health. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In particular, bacteriophage phi 6 is an accessible enveloped virus that can be operated in biosafety level (BSL)-1 as a promising surrogate for SARS-CoV-2 with structural and morphological similarities. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…All experiments were conducted within a BSL-3 laboratory at room temperature. The time required for inactivation of 90 % (T(90)) of SARS-CoV-2 was 10.4, 10.8, and 18.3 h for unfiltered raw, filtered raw, and secondary effluent, respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso