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581por Semeraro, Sabrina, Gaetano, Anastasia Serena, Zupin, Luisa, Poloni, Carlo, Merlach, Elvio, Greco, Enrico, Licen, Sabina, Fontana, Francesco, Leo, Silvana, Miani, Alessandro, Broccolo, Francesco, Barbieri, Pierluigi“…An aerosol generator, chamber and sampler are contained in a class II cabinet in a BSL3 facility. Residual infectivity is assessed from aliquots of liquid collecting bioaerosol, sampled without and with endonasal filters. …”
Publicado 2022
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582por Yu, Xiaolin, Fang, Ming, Wang, Shuang, Li, Zhong, Cheng, Lixiao, Liu, Zhaoshan, Zhang, Dandan, Dong, Dandan, Kou, Zengqiang“…Nucleic acid detection and bacterial isolation and culture in the BSL-2 laboratories. SPSS 21 was used to conduct statistical analysis. …”
Publicado 2022
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583por Queiroz-Junior, Celso M., Santos, Anna C.P.M., Gonçalves, Matheus R., Brito, Camila B., Barrioni, Breno, Almeida, Pedro J., Gonçalves-Pereira, Marcela H., Silva, Tarcília, Oliveira, Sicília R., Pereira, Marivalda M., Santiago, Helton C., Teixeira, Mauro M., Costa, Vivian V.“…In vivo, we evaluated the bone phenotype in a BSL2 mouse model of SARS-like disease induced by murine coronavirus (MHV-3). …”
Publicado 2023
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584por Peppermüller, Pia Pascale, Gehring, Jonathan, Zentrich, Eva, Bleich, André, Häger, Christine, Buettner, Manuela“…Before the induction of intestinal inflammation in one of the animal models, mice underwent an abdominal surgery and the live MGS from the cage side and a clinical score were assessed before (bsl) and after 2, 4, 6, 8, 24, and 48 hours. RESULTS: The highest clinical score as well as the highest live MGS was detected 2 hours after surgery and almost no sign of pain or severity were detected after 24 and 48 hours. …”
Publicado 2023
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585por Barua, Subarna, Bailey, Matthew, Zhong, Kevin, Iduu, Nneka, Dormitorio, Teresa, Macklin, Kenneth, Bourassa, Dianna, Price, Stuart, Hauck, Ruediger, Krehling, James, Kitchens, Steven, Kyriakis, Constantinos, Buhr, R. Jeff, Wang, Chengming“…In this study, 4 groups of SPF Leghorn chickens (n = 50) were orally inoculated with 10(8)Salmonella Enteritidis and 10(8)Campylobacter jejuni, housed in BSL-2 rooms inside containers with autoclaved bedding and beetles (n = 200). …”
Publicado 2023
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586“…Twenty-four hours after AM depletion, we infected mice by aerosolization with a sub-lethal dose of 7.8 x 10(3) CFU/lung of B. thailandensis (Bt), a BSL-2-compliant surrogate model of Bps infection. …”
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587por Schutzer, Steven E., Schlater, Linda R. K., Ronning, Catherine M., DeShazer, David, Luft, Benjamin J., Dunn, John J., Ravel, Jacques, Fraser-Liggett, Claire M., Nierman, William C.“…This exclusion could accelerate research by investigators not possessing BSL-3 facilities and facilitate the production of reagents such as antibodies without the restraints of Select Agent regulation.…”
Publicado 2008
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588por Jado, Isabel, Carranza-Rodríguez, Cristina, Barandika, Jesús Félix, García-Amil, Cristina, Serrano, Beatriz, Bolaños, Margarita, Gil, Horacio, Escudero, Raquel, García-Pérez, Ana L, Olmeda, A Sonia, Astobiza, Ianire, Lobo, Bruno, Rodríguez-Vargas, Manuela, Pérez-Arellano, José Luis, López-Gatius, Fernando, Pascual-Velasco, Francisco, Cilla, Gustavo, Rodríguez, Noé F, Anda, Pedro“…BACKGROUND: Coxiella burnetii is a highly clonal microorganism which is difficult to culture, requiring BSL3 conditions for its propagation. This leads to a scarce availability of isolates worldwide. …”
Publicado 2012
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589por Shafagati, Nazly, Narayanan, Aarthi, Baer, Alan, Fite, Katherine, Pinkham, Chelsea, Bailey, Charles, Kashanchi, Fatah, Lepene, Benjamin, Kehn-Hall, Kylene“…RVFV that was inactivated through either detergent or heat treatment was still found bound to NT53, indicating the ability to use NanoTrap particles for viral capture prior to transport to a BSL-2 environment. Furthermore, both NP-40-lysed virus and purified RVFV RNA were bound by NT53. …”
Publicado 2013
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590por Zhao, Guangyu, Du, Lanying, Ma, Cuiqing, Li, Ye, Li, Lin, Poon, Vincent KM, Wang, Lili, Yu, Fei, Zheng, Bo-Jian, Jiang, Shibo, Zhou, Yusen“…CONCLUSION: Taken together, the established MERS-CoV inhibition assay is a safe and convenient pseudovirus-based alternative to BSL-3 live-virus restrictions and can be used to rapidly screen MERS-CoV entry inhibitors, as well as evaluate vaccine-induced neutralizing antibodies against the highly pathogenic MERS-CoV.…”
Publicado 2013
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591por Hassani Idrissi, Hind, Hmimech, Wiam, Diakite, Brehima, Korchi, Farah, Baghdadi, Dalila, Habbal, Rachida, Nadifi, Sellama“…DNA samples were genotyped by PCR-RFLP method using MboI, BslI and MseI restriction enzymes respectively for the G894T eNOS, 4G/5G PAI and T1131C APOA5 polymorphisms. …”
Publicado 2016
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592por Vazeille, Marie, Gaborit, Pascal, Mousson, Laurence, Girod, Romain, Failloux, Anna-Bella“…We performed oral artificial infections of F1 populations in BSL-3 conditions and analyzed infection, dissemination and transmission rates. …”
Publicado 2016
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593por Chao, Chien-Chung, Zhang, Zhiwen, Belinskaya, Tatyana, Thipmontree, Wilawan, Tantibhedyangkul, Wiwit, Silpasakorn, Saowaluk, Wongsawat, Ekkarat, Suputtamongkol, Yupin, Ching, Wei-Mei“…BACKGROUND: Scrub typhus (ST) is a disease caused by an obligate intracellular bacterium, Orientia tsutsugamushi, an organism that requires a BSL3 laboratory for propagation. The disease is hallmarked by an eschar at the site of the chigger bite, followed by the development of fever, malaise, myalgia, anorexia, and papulomacular rash. …”
Publicado 2017
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594por Eigendorf, Julian, May, Marcus, Friedrich, Jan, Engeli, Stefan, Maassen, Norbert, Gros, Gerolf, Meissner, Joachim D.“…HIHVT was evaluated by comparing performance tests before and after the entire training (baseline, BSL, and endpoint, END) and between the two training sets (intermediate, INT). …”
Publicado 2018
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595por Rogers, Kai J., Brunton, Bethany, Mallinger, Laura, Bohan, Dana, Sevcik, Kristina M., Chen, Jing, Ruggio, Natalie, Maury, Wendy“…METHODS/MAIN FINDINGS: We utilized a BSL2 EBOV model virus, infectious, recombinant vesicular stomatitis virus encoding EBOV glycoprotein (GP) (rVSV/EBOV GP) in place of its native glycoprotein. …”
Publicado 2019
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596por Manopo, Ivanus, Lu, Liqun, He, Qigai, Chee, Li Lian, Chan, Shzu-Wei, Kwang, Jimmy“…Thus, our Spike protein-based IFA could offer a safer procedure which can be performed in a BSL-2 laboratory as it could mimic the whole virus based-IFA without any loss of sensitivity and specificity. …”
Publicado 2005
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597por Kwak, Min Young, Choi, Woo Ri, Park, Jun Woo, Hwang, Eun Jeong, Ha, Yeo Ra, Chung, Jong Woo, Kang, Woo Seok“…All patients used the in-the-canal type of Wide7 hearing aid provided by BSL Co., Ltd. We performed the Korean version of the Hearing Handicap Inventory for the Elderly (K-HHIE) and the International Outcome Inventory for Hearing Aids (K-IOI-HA) before and 1, 3, and 6 months after wearing the hearing aid. …”
Publicado 2020
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598por Wright, Shelton W., Lovelace-Macon, Lara, Ducken, Deirdre, Tandhavanant, Sarunporn, Teparrukkul, Prapit, Hantrakun, Viriya, Limmathurotsakul, Direk, Chantratita, Narisara, West, T. Eoin“…Burkholderia thailandensis (Bt) is a closely related surrogate that does not require BSL-3 conditions for study. Lactoferrin is an iron-binding glycoprotein that can modulate the innate inflammatory response. …”
Publicado 2020
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599por Kaufer, Alexa M., Theis, Torsten, Lau, Katherine A., Gray, Joanna L., Rawlinson, William D.“…Based on the epidemiological and clinical data that have become available in mid-2020, we propose the interim classification of SARS-CoV-2 as a Risk Group 3 organism is reasonable, and discuss establishing Biosafety Level 3 (BSL-3) regulations accordingly. Despite its global spread, the reported mortality rate of SARS-CoV-2 ranging from 0.13% to 6.22% is considerably less than that of other Risk Group 4 agents including Ebola and Marburg viruses with fatality rates as high as 90%. …”
Publicado 2020
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600“…Abbreviations: HA, HAsd, HA stalk domain; L. lactis, Lactococcus lactis; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; IFA, immunofluorescence assay; PBS, phosphate-buffered saline; pgsA, phosphatidylglycerophosphate synthetase A; SPF, specific pathogen-free; CFU, colony-forming unit; BSL-3, biosafety level-3 laboratory; TCID(50), 50% tissue culture infective dose; ELISA, enzyme-linked immunosorbent assay; OD, optical density; LTB, liable enterotoxin B subunit; CTB, cholera toxin B subunit.…”
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