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621por Yu, Fuxun, Du, Yanhua, Huang, Xueyong, Ma, Hong, Xu, Bianli, Adungo, Ferdinard, Hayasaka, Daisuke, Buerano, Corazon C., Morita, Kouichi“…SFTSV has been classified as a BSL3 pathogen. There is a need to develop safe and affordable serodiagnostic methods for proper clinical management of infected patients. …”
Publicado 2015
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622por Norris, Michael H., Rahman Khan, Md Siddiqur, Schweizer, Herbert P., Tuanyok, Apichai“…In the United States, Bp is listed as a Tier-1 select agent by the Centers for Disease Control and Prevention (CDC), thus requiring high levels of regulation and biosafety level 3 (BSL3) facilities for experimental manipulation of live organisms. …”
Publicado 2017
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623“…The primary outcome will be a decrease in the severity of BPD symptoms as assessed by the Borderline Symptom List (BSL-23). For the clinical evaluation, three study assessments will take place: at baseline, after treatment and at 12-month follow-up. …”
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624“…METHODS: A multi-stage prospective cohort study was conducted, where the Prison Screening Questionnaire (PriSnQuest), a modified Borderline Symptom List-23 (BSL-23), Self-Harm Inventory (SHI), Patient Health Questionnaire-9 (PHQ-9) and Clinical Outcomes in Routine Evaluation – Outcome Measure (CORE-OM) instruments were administered to prisoners aged 18 and above, who were judged to be at an increased risk of self-harm (on open ACCT monitoring) during the recruitment phase. …”
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625por Johnston, Gunner P., Bradel-Tretheway, Birgit, Piehowski, Paul D., Brewer, Heather M., Lee, Bom Nae Rin, Usher, Nicholas T., Zamora, J. Lizbeth Reyes, Ortega, Victoria, Contreras, Erik M., Teuton, Jeremy R., Wendler, Jason P., Matz, Keesha M., Adkins, Joshua N., Aguilar, Hector C.“…Classified as a biosafety level 4 (BSL4) select agent, Nipah virus (NiV) is a deadly henipavirus in the Paramyxoviridae family, with a nearly 75% mortality rate in humans, underscoring its global and animal health importance. …”
Publicado 2019
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626por Shi, Shu-Qun, Peng, Jing-Pian, Li, Yin-Chuan, Qin, Chuan, Liang, Guo-Dong, Xu, Li, Yang, Ying, Wang, Jin-Ling, Sun, Quan-Hong“…Virus challenge test was conducted in BSL3 bio safety laboratory with Brandt's vole SARS-CoV model, and the results indicated that co-immunization of M and N antigens could reduce the mortality and pathological changes in lung from the virus infection.…”
Publicado 2006
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627“…There are currently no therapeutics or vaccines available to treat Henipavirus infection and both are classified as BSL4 pathogens. RNA interference (RNAi) is a process by which double-stranded RNA directs sequence-specific degradation of messenger RNA in animal and plant cells. …”
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628por Ludwig-Begall, L.F., Wielick, C., Dams, L., Nauwynck, H., Demeuldre, P-F., Napp, A., Laperre, J., Haubruge, E., Thiry, E.“…It presents advantages of uncomplicated manipulation and utilization in a BSL2 facility, therefore being easily adaptable to other respirator and mask types.…”
Publicado 2020
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629por Nenov-Matt, Tabea, Barton, Barbara B., Dewald-Kaufmann, Julia, Goerigk, Stephan, Rek, Stephanie, Zentz, Katharina, Musil, Richard, Jobst, Andrea, Padberg, Frank, Reinhard, Matthias A.“…Method: Thirty-four PDD patients (DSM-5; 15 female, M(age) = 38.2, SD = 12.3), 36 BPD patients (DSM-5; 19 female, M(age) = 28.8, SD = 9.2), and 70 age- and gender-matched HC were assessed cross-sectionally using the following self-report measures: UCLA Loneliness Scale, Social Network Index (SNI; size, diversity, and embeddedness), Beck Depression Inventory (BDI-II), Borderline Symptom List (BSL-23), Childhood Trauma Questionnaire (CTQ), and Rejection Sensitivity Questionnaire (RSQ). …”
Publicado 2020
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630por Müller, Katharina, Girl, Philipp, von Buttlar, Heiner, Dobler, Gerhard, Wölfel, Roman“…Virus neutralisation tests (NTs) are the gold standard for the detection and quantification of NAbs, but they are complex and require BSL3 facilities. In contrast, surrogate enzyme-linked immunosorbent assays (sELISA) offer the possibility of high-throughput testing under standard laboratory safety conditions. …”
Publicado 2021
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631por Biondi, Mia J., Garnett, Lauren, Bello, Alexander, Funk, Duane, Poliquin, Philippe Guillaume, Jones, Shane, Tierney, Kevin, Tran, Kaylie, Kozak, Robert A., Leung, Anders, Grolla, Allen, Nakamura, Cory, Soule, Geoff, Ranadheera, Charlene, Hagan, Mable, Dhaliwal, Amrinder, Kobasa, Darwyn, Falzarano, Darryl, Bovendo, Hugues Fausther, Feldmann, Heinz, Kesselman, Murray, Hansen, Gregory, Gren, Jason, Mortimer, Todd, Racine, Trina, Deschambault, Yvon, Edmonds, Jocelyn, Aminian, Sam, Saurette, Ray, Allan, Mark, Rondeau, Lauren, Huynh, John, Hadder, Sharron, Press, Christy, DeGraff, Christine, Kucas, Stephanie, Kubay, Julie, Azanarsky, Kim, Cook, Bradley W. M., Hancock, BJ, Kumar, Anand, Soni, Reeni, Schantz, Daryl, McKitrick, Jarrid, Warner, Bryce, Griffin, Bryan D., Qiu, Xiangguo, Kobinger, Gary P., Safronetz, Dave, Wood, Heidi, Stein, Derek R., Cutts, Todd, Pickering, Brad, Kenny, James, Theriault, Steven, Menec, Liam, Vendramelli, Robert, Higgins, Sean, Banadyga, Logan, Liu, Guodong, Rahim, Md Niaz, Kasloff, Samantha, Sloan, Angela, He, Shihua, Tailor, Nikesh, Albietz, Alixandra, Wong, Gary, Gray, Michael, Feldmann, Friederike, Marzi, Andrea, Risi, George, Strong, James E.“…Methods: We created a fully equipped intensive care unit (ICU), within a Biosafety Level 4 (BSL4) laboratory, and infected multiple sedated non-human primates (NHPs) with Ebola virus. …”
Publicado 2021
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632por Konvalin, Franziska, Grosse-Wentrup, Fabienne, Nenov-Matt, Tabea, Fischer, Kai, Barton, Barbara B., Goerigk, Stephan, Brakemeier, Eva-Lotta, Musil, Richard, Jobst, Andrea, Padberg, Frank, Reinhard, Matthias A.“…BPD symptoms were assessed using the Borderline Personality Disorder Severity Index (BPDSI-IV) and the Borderline Symptom List (BSL-23). Intercorrelations of baseline characteristics and symptom change during treatment were analyzed. …”
Publicado 2021
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633por Saleh, Amani A, Saad, Mohamed A, Ryan, Islam, Amin, Magdy, Shindy, Mohamed I, Hassan, Wael A, Samir, Mahmoud, Khattab, Ayman A, Abdelgayed, Sherein S, Seadawy, Mohamed G, Fahmy, Hossam M, Amer, Khaled“…Development of inactivated vaccine was carried out in a BSL-3 facilities and the immunogenicity was determined in mice at two doses (55 and 100 μg per dose). …”
Publicado 2021
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634“…MHV can be manipulated under BSL2 laboratory conditions, unlike SARS-CoV-2, making it a model for studying the virucidal effects on coronaviruses. …”
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635por Caschera, Alexander G., McAuley, Julie, Kim, Youry, Purcell, Damian, Rymenants, Jasper, Foucher, Daniel A.“…METHODS: Two studies were performed on antimicrobial pre-treated metal disks in March 2020 by two independent Biosafety Level III (BSL-3) equipped laboratories in April 2020. These facilities were in Belgium (the Rega Medical Research Institute) and Australia (the Peter Doherty Institute) and independently applied quantitative carrier-based methodologies using the authentic SARS-CoV-2 isolates (hCoV-19/Australia/VIC01/2020, hCoV-19/Belgium/GHB-03021/2020). …”
Publicado 2022
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636por Solastie, Anna, Virta, Camilla, Haveri, Anu, Ekström, Nina, Kantele, Anu, Miettinen, Simo, Lempainen, Johanna, Jalkanen, Pinja, Kakkola, Laura, Dub, Timothée, Julkunen, Ilkka, Melin, Merit“…Neutralization tests are still considered the gold standard for SARS-CoV-2 serological assays, but our assay can identify samples with neutralizing antibodies with 100% sensitivity and 96% specificity without the need for laborious and slow biosafety level 3 (BSL-3) facility-requiring analyses.…”
Publicado 2021
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637por Zeng, Jianxiong, Xie, Xiaochun, Feng, Xiao-Li, Xu, Ling, Han, Jian-Bao, Yu, Dandan, Zou, Qing-Cui, Liu, Qianjin, Li, Xiaohong, Ma, Guanqin, Li, Ming-Hua, Yao, Yong-Gang“…METHODS: We infected THP-1 derived macrophages, NLRP3 knockout mice, and human ACE2 transgenic mice with live SARS-CoV-2 in Biosafety Level 3 (BSL-3) laboratory. We performed quantitative real-time PCR for targeted viral or host genes from SARS-CoV-2 infected mouse tissues, conducted histological or immunofluorescence analysis in SARS-CoV-2 infected mouse tissues. …”
Publicado 2021
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638por DeFina, Samuel M., Wang, Jianhui, Yang, Lei, Zhou, Han, Adams, Jennifer, Cushing, William, Tuohy, Beth, Hui, Pei, Liu, Chen, Pham, Kien“…This novel, two-step, RT-LAMP assay utilizes a customized multiplex primer set specifically targeting SARS-CoV-2 and a visual report system that is ready to interpret within 40 min from the start of sample processing and does not require a BSL-2 level testing environment or special laboratory equipment. …”
Publicado 2022
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639por Singletary, Melissa, Krichbaum, Sarah, Passler, Thomas, Lazarowski, Lucia, Fischer, Terrence, Silvis, Scott, Waggoner, L. Paul, Walz, Paul, Angle, Craig“…The restrictive nature for mimicking the access and handling of a BSL 3+ agent presented challenges that required a strict study design uncommon to standard detection dog training and odor presentation. …”
Publicado 2022
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640por Elijah, Catherine Grace, Trujillo, Jessie D., Jones, Cassandra K., Kwon, Taeyong, Stark, Charles R., Cool, Konner R., Paulk, Chad B., Gaudreault, Natasha N., Woodworth, Jason C., Morozov, Igor, Gallardo, Carmina, Gebhardt, Jordan T., Richt, Jürgen A.“…Batches of feed were manufactured in a BSL‐3Ag room at Kansas State University's Biosafety Research Institute in Manhattan, Kansas. …”
Publicado 2021
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