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1801“…CONCLUSION: Our experimental results suggested that the protonophores-mediated accumulation and aggregation of membrane proteins (like AP) in cell cytosol had signaled the induction of heat-shock proteins in E. coli and the non-translocated protein aggregates were possibly degraded by an induced heat-shock protease ClpP. Moreover, the induction of heat-shock response occurred by the stabilization of sigma-32. …”
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1802por Schick, Martin Alexander, Isbary, Jobst Tobias, Stueber, Tanja, Brugger, Juergen, Stumpner, Jan, Schlegel, Nicolas, Roewer, Norbert, Eichelbroenner, Otto, Wunder, Christian“…METHODS: Sepsis was induced by cecal ligation and puncture (CLP) in 77 male rats. Animals were treated with different crystalloids (NaCl 0.9% (NaCl), Ringer’s acetate (RA)) or colloids (Gelafundin 4% (Gel), 6% HES 130/0.4 (HES)). …”
Publicado 2012
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1803por Calisto, Kelly L, Camacho, Angélica C, Mittestainer, Francine C, Carvalho, Bruno M, Guadagnini, Dioze, Carvalheira, José B, Saad, Mario J“…METHODS: Diffuse sepsis was induced by cecal ligation and puncture surgery (CLP) in male Wistar rats. Blood glucose and inflammatory cytokine levels were assessed 24 hours after CLP. …”
Publicado 2012
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1804por Bhargava, Rhea, Altmann, Christopher J., Andres-Hernando, Ana, Webb, Ryan G., Okamura, Kayo, Yang, Yimu, Falk, Sandor, Schmidt, Eric P., Faubel, Sarah“…RESULTS: Serum cytokines increased with all 5 doses of LPS; AKI and ALI were detected within 4 hours of IP LPS or CLP, using sensitive markers of GFR and lung inflammation, respectively. …”
Publicado 2013
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1805por Khailova, Ludmila, Petrie, Benjamin, Baird, Christine H., Dominguez Rieg, Jessica A., Wischmeyer, Paul E.“…OBJECTIVE: Evaluate if Lactobacillus rhamnosus GG (LGG) or Bifidobacterium longum (BL) treatment in a weanling mouse model of cecal ligation and puncture (CLP) peritonitis will protect against lung injury. …”
Publicado 2014
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1806por Kadowaki, Takashi, Morishita, Asahiro, Niki, Toshiro, Hara, Junko, Sato, Miwa, Tani, Joji, Miyoshi, Hisaaki, Yoneyama, Hirohito, Masaki, Tsutomu, Hattori, Toshio, Matsukawa, Akihiro, Hirashima, Mitsuomi“…METHODS: We induced polymicrobial sepsis by cecal ligation and puncture (CLP) in mice. The survival rate was compared between galectin-9- and PBS-treated CLP mice. …”
Publicado 2013
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1807por Chen, Qi Xing, Song, Sheng Wen, Chen, Qing Hua, Zeng, Cong Li, Zheng, Xia, Wang, Jun Lu, Fang, Xiang Ming“…METHODS: Acute lung injury was induced by polymicrobial sepsis via cecal ligation and puncture (CLP) surgery. Adenovirus-mediated short hairpin RNA specific for the mouse hepcidin gene hepc1 and control adenovirus were intratracheally injected into mice. …”
Publicado 2014
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1808por Clavier, Thomas, Tonon, Marie-Christine, Foutel, Anne, Besnier, Emmanuel, Lefevre-Scelles, Antoine, Morin, Fabrice, Gandolfo, Pierrick, Tuech, Jean-Jacques, Quillard, Muriel, Veber, Benoit, Dureuil, Bertrand, Castel, Hélène, Compère, Vincent“…Measurements of plasma DBI-related peptides were carried out by radioimmunoassay in animal and human samples. RESULTS: In the rats, CLP provoked an increase of plasma DBI-LI (+37%) 6 hours postsurgery. …”
Publicado 2014
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1809por Benz, Fabian, Tacke, Frank, Luedde, Mark, Trautwein, Christian, Luedde, Tom, Koch, Alexander, Roderburg, Christoph“…We used i.v. injection of lipopolysaccharide (LPS) as well as cecal pole ligation and puncture (CLP) for induction of polymicrobial sepsis in mice and measured alterations in serum levels of miR-223. …”
Publicado 2015
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1810por Acosta-Herrera, Marialbert, Lorenzo-Diaz, Fabian, Pino-Yanes, Maria, Corrales, Almudena, Valladares, Francisco, Klassert, Tilman E., Valladares, Basilio, Slevogt, Hortense, Ma, Shwu-Fan, Villar, Jesus, Flores, Carlos“…Sepsis was induced by cecal ligation and puncture (CLP) in male Sprague-Dawley rats. At 24 hours post-CLP, septic animals were randomized to three ventilatory strategies: spontaneous breathing, LVT (6 ml/kg) plus 10 cmH(2)O PEEP and high tidal volume (HVT, 20 ml/kg) plus 2 cmH(2)O PEEP. …”
Publicado 2015
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1811por Wu, Nan, He, Lei, Cui, Peng, Wang, Wenjie, Yuan, Youhua, Liu, Shuang, Xu, Tao, Zhang, Shanshan, Wu, Jing, Zhang, Wenhong, Zhang, Ying“…We found that oxyR, dnaK, sucB, relA, rpoS, clpB, mqsR, and recA were prominent persister genes involved in persistence to multiple antibiotics. …”
Publicado 2015
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1812“…We aimed to investigate the effects of sepsis on the antagonistic actions of neostigmine on rocuronium (Roc) and the underlying changes of AChE activity in the NMJ in a rat model of cecal ligation and puncture (CLP). METHODS: A total of 28 male adult Sprague-Dawley rats were randomized to undergo a sham surgery (the sham group, n = 12) or CLP (the septic group, n = 16). …”
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1813por Kim, Jung Wook, Kim, Hyun-Kyung, Kang, Gi Su, Kim, Il-Hwan, Kim, Hwa Su, Lee, Yeong Seon, Yoo, Jae Il“…Microarray and proteomic analyses show that the expression of cell-wall-associated genes glmS and murZ are lower, and the expression of heat shock and stress-related genes (hrcA, ctsR, dnaK, dnaJ, grpE, clpB, and clpC) are higher in the sav1322 mutant when compared with the wild-type strain. …”
Publicado 2016
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1814por Danahy, Derek B., Anthony, Scott M., Jensen, Isaac J., Hartwig, Stacey M., Shan, Qiang, Xue, Hai-Hui, Harty, John T., Griffith, Thomas S., Badovinac, Vladimir P.“…IFN-γ-mediated recruitment of immune cells to the site of localized infection was, however, reduced in CLP hosts despite T(RM) maintaining their ‘sensing and alarming’ functions. …”
Publicado 2017
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1815por Arslan, Demet, Ekinci, Aysun, Arici, Akgul, Bozdemir, Eda, Akil, Esref, Ozdemir, Hasan Huseyin“…Rats were subjected to CLP except for the control group, which underwent laparatomy only. …”
Publicado 2017
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1816por Bærnthsen, Nina Falcon, Hansen, Marco Bo, Wahl, Anna Mygind, Simonsen, Ulf, Hyldegaard, Ole“…We investigated the effects of 24 h delayed normobaric oxygen (NBO(2)) and HBO(2) treatment on the endogenous production of the inflammatory markers interleukin (IL)-6, tumor necrosis factor (TNF)-α and IL-10, and on mortality in rats with cecal ligation and puncture (CLP) induced sepsis. METHOD: Fifty-five male Sprague-Dawley rats underwent CLP and were randomized to the following groups: 1) HBO(2) 2.5 bar absolute pressure (p(abs)); 2) NBO(2) 1.0 bar p(abs); 3) Control (no-treatment), and they were individually monitored for 72 h with intermittent blood sampling. …”
Publicado 2017
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1817por Zhao, Yarong, Zhu, Haiyan, Wang, Haining, Ding, Liang, Xu, Lizhi, Chen, Dai, Shen, Sunan, Hou, Yayi, Dou, Huan“…Moreover, let-7a-5p expression was abrogated upon CLP stimulation in vivo, whereas it was restored by FC-99 treatment. …”
Publicado 2018
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1818por Aziz, Monowar, Ode, Yasumasa, Zhou, Mian, Ochani, Mahendar, Holodick, Nichol E., Rothstein, Thomas L., Wang, Ping“…METHODS: Sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP). PBS or B-1a cells were adoptively transferred into the septic mice intraperitoneally. …”
Publicado 2018
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1819por Babic, Zivan M., Zunic, Filip Z., Pantic, Jelena M., Radosavljevic, Gordana D., Jovanovic, Ivan P., Arsenijevic, Nebojsa N., Lukic, Miodrag L.“…METHODS: Polymicrobial sepsis was induced using cecal ligation and puncture (CLP) model in ST2 deficient (ST2(−/−)) and wild type BALB/c mice. …”
Publicado 2018
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1820“…Five overlapping proteins were differentially expressed in both the oeCIPK14-var and why1/3-var lines: ATP-dependent Clp protease proteolytic subunit-related protein 3 (ClpR3), Ribulose bisphosphate carboxylase large chain (RBCL), Beta-amylase 3 (BAM3), Ribosome-recycling factor (RRF), and Ribulose bisphosphate carboxylase small chain (RBCS). …”
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