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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
1
ácido diclorofenoxiacético
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361“…The highest fresh weight of callus was produced on leaf explants in the presence of 2,4-D or PIC. …”
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362por Amemiya, Ayumi, Noguchi, Hiroshi, Oe, Makoto, Takehara, Kimie, Ohashi, Yumiko, Suzuki, Ryo, Yamauchi, Toshimasa, Kadowaki, Takashi, Sanada, Hiromi, Mori, Taketoshi“…Callus has been identified as a risk factor leading to severe diabetic foot ulcer; thus, it is necessary to prevent its formation. …”
Publicado 2020
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363por Ren, Li, Deng, Shan, Chu, Yunxia, Zhang, Yiying, Zhao, Hong, Chen, Hairong, Zhang, Di“…RESULTS: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. …”
Publicado 2020
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364por Wen, Li, Li, Wei, Parris, Stephen, West, Matthew, Lawson, John, Smathers, Michael, Li, Zhigang, Jones, Don, Jin, Shuangxia, Saski, Christopher A.“…The critical genetic architecture of non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells in a highly regenerable cotton genotype is unknown. …”
Publicado 2020
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365“…Adopting live-cell imaging with bright-field, fluorescent and confocal microscopy and bacterial 16S-rRNA gene taxonomic profiling, we report the cytoplasmic association of abundant and diverse CREBs in long-term actively maintained callus and cell suspension cultures of different plant species. …”
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366por Kok, Andrew De-Xian, Mohd Yusoff, Nur Fatihah, Sekeli, Rogayah, Wee, Chien-Yeong, Lamasudin, Dhilia Udie, Ong-Abdullah, Janna, Lai, Kok-Song“…Hence, this study was undertaken to elucidate the growth-promoting mechanism of PF-68 using recalcitrant MR 219 callus as a model. Supplementation of 0.04% PF-68 (optimum concentration) was shown to enhance callus proliferation. …”
Publicado 2021
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367“…For this reason, we used whole-genome bisulphite sequencing, mRNA sequencing and small RNA sequencing to study the epigenetic status and expression of TEs in embryogenic callus, in comparison with leaf tissue. RESULTS: We found that compared with leaf tissue, grapevine embryogenic callus cultures accumulate relatively high genome-wide CHH methylation, particularly across heterochromatic regions. …”
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368por Zhang, Sufang, Yan, Shanshan, An, Peiqi, Cao, Qing, Wang, Chen, Wang, Junhui, Zhang, Hanguo, Zhang, Lei“…In this study, the transformation rate of resistance callus was 32.1%. It provides a reference for low genetic transformation efficiency of larch at present. …”
Publicado 2021
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369por Khan, Habiba, Khan, Tariq, Ahmad, Nisar, Zaman, Gouhar, Khan, Taimoor, Ahmad, Waqar, Batool, Sannia, Hussain, Zahid, Drouet, Samantha, Hano, Christophe, Abbasi, Bilal Haider“…Therefore, this study was designed to investigate the effects of chemical elicitors, aluminum chloride (AlCl(3)) and cadmium chloride (CdCl(2)), on the biosynthesis of secondary metabolites, biomass, and the antioxidant system in callus cultures of F. indica. Among various treatments applied, AlCl(3) (0.1 mM concentration) improved the highest in biomass accumulation (fresh weight (FW): 404.72 g/L) as compared to the control (FW: 269.85 g/L). …”
Publicado 2021
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370por Li, Yingbo, Guo, Guimei, Xu, Hongwei, He, Ting, Zong, Yingjie, Zhang, Shuwei, Faheem, Muhammad, Lu, Ruiju, Zhou, Longhua, Liu, ChenghongEnlace del recurso
Publicado 2021
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371“…This study is a first extensive report about the effect of different serialization protocols and plant growth regulators (PGRs) on explant disinfection efficiency, callus induction and secondary metabolites production and accumulation in callus cultures of M. azedarach L. …”
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372“…METHODS: Four explants were used to induce callus using 2,4-D and IBA at 1, 2, 3 and 4 mg/l for each auxin. …”
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373por Kirakosyan, Rima N., Sumin, Anton V., Polupanova, Anna A., Pankova, Maria G., Degtyareva, Irina S., Sleptsov, Nikolay N., Khuat, Quyet V.“…Light conditions and plant growth regulators (PGRs) are two of many factors that affect the growth and inulin content of chicory callus. The aim of this work is to study the effect of PGRs and light conditions on proliferation and accumulation of inulin of chicory callus in vitro. …”
Publicado 2022
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374por Deng, Jinwu, Sun, Weinan, Zhang, Boyang, Sun, Simin, Xia, Linjie, Miao, Yuhuan, He, Liangrong, Lindsey, Keith, Yang, Xiyan, Zhang, Xianlong“…Wounded plant cells can form callus to seal the wound site. Alternatively, wounding can cause adventitious organogenesis or somatic embryogenesis. …”
Publicado 2022
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375por Taratima, Worasitikulya, Chuanchumkan, Chantima, Maneerattanarungroj, Pitakpong, Trunjaruen, Attachai, Theerakulpisut, Piyada, Dongsansuk, Anoma“…Dehusked seeds were sterilized and cultured on Murashige and Skoog (MS) medium, supplemented with 3 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) for callus induction. The cultures were maintained at 25 °C and 35 °C for 4 weeks, while the other culture was treated with heat shock at 42 °C for 1 week before further incubation at 25 °C for 3 weeks. …”
Publicado 2022
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376por Yoshimoto, Naoko, Asano, Takashi, Kisanuki, Ayuna, Kanno, Chihiro, Asanuma, Machiko, Yamazaki, Mami, Fujii, Isao, Saito, Kazuki“…Callus tissues were initiated and maintained in the presence of picloram and 2-isopentenyladenine as auxin and cytokinin, respectively. …”
Publicado 2022
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377por Fu, Yongyao, Shu, Liling, Li, Hanyi, Zhang, Xingming, Liu, Xuan, Ou, Zhengying, Liang, Xiaomeng, Qi, Xiangying, Yang, Liping“…In this study, the highly efficient regeneration of Longya lily was established through the optimization of embryogenic callus, adventitious shoot and rooting induction. …”
Publicado 2023
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378por Nam, Dong-Geon, Yang, Hee-Sun, Bae, Ui-Jin, Park, Eunmi, Choi, Ae-Jin, Choe, Jeong-Sook“…In a molecular docking study, selected compounds from both Opuntia ficus-indica callus (OFC) and OF ethanol extract were found to be involved in Toll-like receptor 4 and mitogen-activated protein kinase (MAPK) signaling pathways. …”
Publicado 2023
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379por Lee, Ka Youn, Shin, Ju Young, Ahn, Myung Suk, Kim, Se Jin, An, Hye Ryun, Kim, Yae Jin, Kwon, O Hyeon, Lee, Su Young“…In addition, the VOCs contained in the petals of 15R-12-2 and the petal-derived callus were analyzed by HS-SPME-GC-MS. Thirty components, including esters and alcohols, were detected in the petal-derived callus. …”
Publicado 2023
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380por Daniela, Lulli, Alla, Potapovich, Maurelli, Riccardo, Elena, Dellambra, Giovanna, Pressi, Vladimir, Kostyuk, Roberto, Dal Toso, Chiara, De Luca, Saveria, Pastore, Liudmila, Korkina“…In nature, the plant is sparsely available and protected; therefore production of callus cultures was established. A concentrated ethanolic extract of culture homogenate, with leontopodic acid representing 55 ± 2% of the total phenolic fraction (ECC55), was characterized for anti-inflammatory properties in primary human keratinocytes (PHKs) and endotheliocytes (HUVECs). …”
Publicado 2012
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