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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
1
ácido diclorofenoxiacético
1
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21“…OBJECTIVE: Our study was focused on devising an optimum procedure for callus induction and phenolic compounds production in N. binaloudensis. …”
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22“…RESULTS: Evidence was sought as to whether the frequency of homoeologous silencing in in vitro cultured wheat callus differ from that in differentiated organs, given that disorganized cells are associated with a globally lower level of DNA methylation. …”
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24“…Callus was initiated from zygotic embryos after 10–20 d culture on MS media supplemented with 4.0 mg/L 2, 4-D and 0.1 mg/L zeatin (ZT). …”
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25por Lipinsky, Pawel V., Sirotin, Ivan V., Skoroglyadov, Alexandr V., Ivkov, Alexey V., Oettinger, Alexandr P., Krynetskiy, Evgeny E., But-Gusaim, Alexandr B., Roth, Andreas J.“…METHODS: Data were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. …”
Publicado 2015
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26por Park, Ji-Sun, Seong, Zuh-Kyung, Kim, Mi-Sun, Ha, Jang-Ho, Moon, Ki-Beom, Lee, Hyo-Jun, Lee, Hyeong-Kyu, Jeon, Jae-Heung, Park, Sang Un, Kim, Hyun-Soon“…The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. …”
Publicado 2020
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27por Abdelsalam, Nader R., Grad, Wafaa E., Ghura, Nabawya S.A., Khalid, Ahmed E., Ghareeb, Rehab Y., Desoky, El-Sayed M., Rady, Mostafa M., Al-Yasi, Hatim M., Ali, Esmat F.“…For inducing sugarcane callus, the medium of Murashige and Skoog (MS) was used with different mannitol concentrations (100, 200, and 300 mM) to measure their effects on callus frequency, the day of callus initiation, embryogenic potential, relative growth rate (RGR), water and proline contents, K(+) and Na(+) contents, as well as the formation of shoot and roots for three sugarcane genotypes (e.g., GT 54-9, G 84-47, and pH 8013). …”
Publicado 2021
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28“…The manual monitoring of callus with digital radiography (X-ray) is the primary bone healing evaluation, assessing the number of bridged callus formations. …”
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29“…At present, somatic embryogenesis (SE) is the most effective method of micro-proliferation in conifers, but the induction rate of embryogenic callus (EC) is low, and EC is difficult to differentiate from non-embryonic callus (NEC). …”
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30por Yoshida, Takashi, Kim, Wook-Cheol, Oka, Yoshinobu, Yamada, Naotake, Kubo, Toshikazu“…BACKGROUND AND PURPOSE: Evaluation of distraction callus is important for determination of the optimal time for removal of external fixation. …”
Publicado 2010
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31por Carciofi, Massimiliano, Blennow, Andreas, Nielsen, Morten M, Holm, Preben B, Hebelstrup, Kim H“…A Green Fluorescent Protein reporter protein tag was used to identify and propagate only fully transgenic callus explants. Around 1 – 1.5 g dry weight of fully transgenic callus could be produced in 9 weeks. …”
Publicado 2012
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32“…Sericostoma pauciflorum Stocks ex Wight (Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). …”
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33por Zheng, Wu, Zhang, Xueyan, Yang, Zuoren, Wu, Jiahe, Li, Fenglian, Duan, Lanling, Liu, Chuanliang, Lu, Lili, Zhang, Chaojun, Li, Fuguang“…Upland cotton (Gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. Callus from only a few cultivars can produce embryogenic callus (EC), but the mechanism is not well elucidated. …”
Publicado 2014
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35por Chevala, Naga Pavan Kumar, Chevala, Naga Thirumalesh, Dhanakodi, Kirubakaran, Nadendla, Rama Rao, Nagarathna, Chandrashekara Krishnappa“…In the present research we studied the accumulation of polyphenols of in vitro regenerated callus from anthers. OBJECTIVE: Callus induction of tea anthers and in vitro accumulation of phenolic compounds from the anther-derived callus. …”
Publicado 2016
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36“…Murashige and Skoog (MS) medium supplemented with 3 mg/l 2, 4-dichlorophenoxyacetic acid, 1 mg/l benzyladenine (BA), 500 mg/l glutamine, 500 mg/l proline, and 500 mg/l casein hydrolysate yielded the best rates of callus induction and granular-compact callus induction. …”
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37por Bissinger, Oliver, Götz, Carolin, Wolff, Klaus-Dietrich, Hapfelmeier, Alexander, Prodinger, Peter Michael, Tischer, Thomas“…In the future, contrast-enhanced μCT imaging of the callus cartilage might provide more information to improve the non-destructive and non-invasive prediction of callus mechanical properties. …”
Publicado 2017
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39por Tuskan, Gerald A., Mewalal, Ritesh, Gunter, Lee E., Palla, Kaitlin J., Carter, Kelsey, Jacobson, Daniel A., Jones, Piet C., Garcia, Benjamin J., Weighill, Deborah A., Hyatt, Philip D., Yang, Yongil, Zhang, Jin, Reis, Nicholas, Chen, Jin-Gui, Muchero, Wellington“…A characteristic feature of plant cells is the ability to form callus from parenchyma cells in response to biotic and abiotic stimuli. …”
Publicado 2018
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40“…Sucrose treatment had various effects on leaves callusing, but had no effect on petioles callusing, whereby 4% sucrose was the best treatment for leaves callusing in dark incubation. …”
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