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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
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ácido diclorofenoxiacético
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521“…Investigations were carried out to determine antimicrobial and antioxidant properties and total phenol content of three wild species of Ephedra compared with their respective callus cultures. Callus induction was performed in a standard Murashige and Skoog (MS) medium with the following hormonal ranges (mg/L) for every species NAA:1.5, Kin:1 for Ephedra strobiliacea, NAA:2, Kin:1 for Ephedra procera and NAA:2, Kin:0.5 for Ephedra pachyclada. …”
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522por Colucci, Silvia Concetta, Buccoliero, Cinzia, Sanesi, Lorenzo, Errede, Mariella, Colaianni, Graziana, Annese, Tiziana, Khan, Mohd Parvez, Zerlotin, Roberta, Dicarlo, Manuela, Schipani, Ernestina, Kozloff, Kenneth M., Grano, Maria“…Osteoclast count within the callus showed a 2.4-fold increase compared with untreated mice (p = 0.026), indicating a more advanced stage of endochondral ossification of the callus during the early stage of fracture repair. …”
Publicado 2021
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523por Ming, NG Ja, Binte Mostafiz, Suraiya, Johon, Nur Syafiqoh, Abdullah Zulkifli, Nur Saliha, Wagiran, Alina“…Scanning electron microscopy and histological analysis were performed to identify the embryogenic callus for regeneration. The callus percentage results showed that MS (Murashige and Skoog) basal medium supplemented with 3 mg/L 2, 4-D and 30g/L maltose were the optimal callus induction medium for MR220 (80%) and MR220-CL2 (95%). …”
Publicado 2019
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524“…The optimal inflorescence-derived callus induction was observed on MP medium (MS medium supplemented with 2 g L(−1) L-proline, 3% maltose, 5 mg L(−1) 2,4-D, and 500 μg L(−1) BAP), resulting in callus induction of 100 ± 0.0% for FIL2 and 84 ± 2.4% for HAL2. …”
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525por Chen, Hangang, Sun, Xianding, Yin, Liangjun, Chen, Shuai, Zhu, Ying, Huang, Junlan, Jiang, Wanling, Chen, Bo, Zhang, Ruobin, Chen, Lin, Nie, Mao, Xie, Yangli, Deng, Zhongliang“…Bone fracture healing is processed through multiple stages including the cartilaginous callus formation and its transition to bony callus. …”
Publicado 2017
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526“…Moreover, this callus formation continued for 8 weeks and led to healing of the fracture. 2) Abundant callus formation was found circumferentially around the cortex with a ‘cloud-like’ appearance. 3) Remodeling of the teriparatide-induced callus formation was found to be part of the normal fracture healing process. …”
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527por Graef, F., Seemann, R., Garbe, A., Schmidt-Bleek, K., Schaser, K-D., Keller, J., Duda, G., Tsitsilonis, S.“…Patients with traumatic brain injury (TBI) and long-bone fractures can show increased callus formation. This effect has already been reproduced in wild-type (wt) mice. …”
Publicado 2017
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528por Wang, Fei, Han, Lihua, Wang, Xiaohong, Li, Yuanyuan, Zhu, Yujie, Wang, Jingfeng, Xue, Changhu“…Dynamic histological analysis showed that Ca-SGP promoted the generation of cartilage callus on day 5 post-surgery, then facilitated the transformation of the cartilage callus to bony callus on days 11 and 24 post-surgery, and enhanced the remodeling of bony callus on 35 day post-surgery. …”
Publicado 2017
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529por Ren, Yongxin, Liu, Bo, Feng, Yuxu, Shu, Lei, Cao, Xiaojian, Karaplis, Andrew, Goltzman, David, Miao, Dengshun“…The mineralized callus areas, bony callus areas, osteoblast number and activity, osteoclast number and surface in callus tissues were all reduced in vehicle-treated and PTH-treated Pth (−/−) mice compared to vehicle-treated and PTH-treated wild-type mice, but were increased in PTH-treated wild-type and Pth (−/−) mice compared to vehicle-treated wild-type and Pth (−/−) mice. …”
Publicado 2011
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530por Wang, Weixi, Nyman, Jeffry S, Moss, Heather E, Gutierrez, Gloria, Mundy, Gregory R, Yang, Xiangli, Elefteriou, Florent“…Callus strength measured by three-point bending 28 days after fracture was reduced in [Image: see text] versus wild-type calluses. …”
Publicado 2010
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531por Natoli, Roman M., Yu, Henry, Meislin, Megan Conti-Mica, Abbasnia, Pegah, Roper, Philip, Vuchkovska, Aleksandra, Xiao, Xianghui, Stock, Stuart R., Callaci, John J.“…Our laboratory has previously shown that episodic alcohol exposure of rodents negatively affects fracture callus development, callus biomechanics, and cellular signaling which regulates stem cell differentiation. …”
Publicado 2018
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532por Lu, Ruiju, Chen, Zhiwei, Gao, Runhong, He, Ting, Wang, Yifei, Xu, Hongwei, Guo, Guimei, Li, Yingbo, Liu, Chenghong, Huang, Jianhua“…The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease the callus induction. …”
Publicado 2016
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533por Khan, Atta Ullah, Khan, Tariq, Khan, Mubarak Ali, Nadhman, Akhtar, Aasim, Muhammad, Khan, Nadir Zaman, Ali, Waqar, Nazir, Nausheen, Zahoor, Muhammad“…The callus cultures of Fagonia indica could prove as factories for the production of important phytochemicals when triggered through different types of stress. …”
Publicado 2021
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534“…Thus, the callus was treated for 60 min and was found to induce the mutation on the callus. …”
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535por Pramanik, Dewi, Dorst, Nemi, Meesters, Niels, Spaans, Marlies, Smets, Erik, Welten, Monique, Gravendeel, Barbara“…Early stage and mature tissues were collected from flowers of the bee-pollinated Phalaenopsis equestris and Phalaenopsis pulcherrima, two species that differ in floral morphology: P. equestris has a large callus but short stelidia and no mentum, whereas P. pulcherrima has a small callus, but long stelidia and a pronounced mentum. …”
Publicado 2020
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536Genetic analysis of reciprocal differences in the inheritance of in vitro characters in pearl milletpor Satyavathi, Valluri V, Manga, V., Rao, Muktinutalapati V. Subba, Chittibabu, Malladi“…Additive-maternal effects influenced total callus quantity and dominance-maternal effects influenced total callus quantity, embryogenic callus quantity and regeneration frequency. …”
Publicado 2016
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537“…Second, we cultured calluses using plant tissue culture technology, from Perilla leaf explant and then examined the effects of the leaf and callus extracts of Perilla on UVB exposed keratinocytes. …”
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538“…In addition to bone surfaces and the growth plate, COX-2 expressing osteoclasts were localized at the chondro-osseous junction of the fracture callus. These observations suggest that arachidonic acid mediated signaling from callus chondrocytes or from callus osteoclasts at the chondro-osseous junction regulate fracture healing.…”
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539por Shim, Sangrea, Kim, Hee Kyoung, Bae, Soon Hyung, Lee, Hoonyoung, Lee, Hyo Ju, Jung, Yu Jin, Seo, Pil Joon“…In vitro plant regeneration involves a two-step practice of callus formation and de novo organogenesis. During callus formation, cellular competence for tissue regeneration is acquired, but it is elusive what molecular processes and genetic factors are involved in establishing cellular pluripotency. …”
Publicado 2020
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540por Awad, Khairullah M., Salih, Ansam M., Khalaf, Yahya, Suhim, Aqeel A., Abass, Mohammed Hamza“…At high Al concentration (200 mg.l(-1)), the callus failed to produce any embryo. Biochemical analysis revealed that Al exposure had negative effect on callus. …”
Publicado 2019
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