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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
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ácido diclorofenoxiacético
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41“…High callus production is a feasible way to improve the propagation coefficient of garlic. …”
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42por Deng, Xianbao, Xiong, Yaqian, Li, Jing, Yang, Dong, Liu, Juan, Sun, Heng, Song, Heyun, Wang, Yunmeng, Ma, Junyu, Liu, Yanling, Yang, Mei“…Here, we developed a highly efficient callus induction system for the lotus by optimizing a series of key factors that affect callus formation. …”
Publicado 2020
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43por Grima, Joseph N., Vella Wood, Michelle, Portelli, Nadia, Grima-Cornish, James N., Attard, Daphne, Gatt, Alfred, Formosa, Cynthia, Cerasola, Dario“…Results: It was found that approximately 69% of rowers participating in this study have calluses on their hands for most of their time (considered by them as not painful). …”
Publicado 2022
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44“…Despite a wealth of knowledge about fracture repair, especially gene and more recently miRNA expression, much remains unknown about the molecular differences between normal physiological repair (callus tissue) and a nonunion. To probe this lack of knowledge, we embarked on a study that sought to identify and compare the human miRNAome of normal bone to that present in a normal fracture callus and those from two different classic nonunion types, hypertrophic and oligotrophic. …”
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45por Park, Joo-Seok, Choi, Yoram, Jeong, Min-Gyun, Jeong, Yeong-Il, Han, Ji-Hyun, Choi, Hong-Kyu“…Callus, a valuable tool in plant genetic engineering, originates from dedifferentiated cells. …”
Publicado 2023
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46“…We evaluated regeneration parameters such as the percentage of callus formation and the area of callus formed, as well as the initiation percentage and the number of adventitious shoots. …”
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47Publicado 1976“…Cultured callus tissue of hazel (Corylus avellana L.), which has the potency of somatic embryogenesis, was used for the study of cell ultrastructure in the course of callus growth and embryoid formation. …”
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48por McDonald, Stuart J, Dooley, Philip C, McDonald, Aaron C, Schuijers, Johannes A, Ward, Alex R, Grills, Brian L“…BACKGROUND AND PURPOSE: We have previously shown that early fracture callus of rat rib has viscoelastic and contractile properties resembling those of smooth muscle. …”
Publicado 2012
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49“…Picrolam (10μM) has shown 36.6% response and the average weight of callus was less as compared with 2,4-D. The callus obtained was friable and opaque in nature.…”
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50“…Successful callusing was obtained from leaf segments of Gymnema sylvestre R. …”
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51“…The MS medium supplemented with 2, 4-D (6.78 μm) was found most suitable for callus induction in petiole explant. The best growth response and higher rate of shoot regeneration from petiole callus was observed on MS medium containing BAP (11.10 μm) as the average number of shoots could be increased to 36.4 on fourth successive subculturing. …”
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52por Kumari, Alka, Ray, Kamalika, Sadhna, Sadhna, Pandey, Arun Kumar, Sreelakshmi, Yellamaraju, Sharma, Rameshwar“…However, the metabolic shifts involved in callus formation and shoot regeneration are largely unknown. …”
Publicado 2017
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54por Zhao, Tingting, Wang, Zemin, Su, Lingye, Sun, Xiaoming, Cheng, Jun, Zhang, Langlang, Karungo, Sospeter Karanja, Han, Yuepeng, Li, Shaohua, Xin, Haiping“…Several parameters affecting the transformation efficiency were optimized including the concentration of kanamycin, Agrobacterium stains, bacterial densities, infection treatments and co-cultivation time. The transgenic callus lines were verified by checking the integration of NPTII gene into calli genomes, the expression of eGFP gene and the fluorescence of eGFP. …”
Publicado 2017
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55por Ge, Fei, Huang, Xing, Hu, Hongmei, Zhang, Yanling, Li, Zhaoling, Zou, Chaoying, Peng, Huanwei, Li, Lujiang, Gao, Shibin, Pan, Guangtang, Shen, Yaou“…The induction efficiency of maize embryonic callus is highly dependent on the genotype, and only a few lines possess a high capacity for callus formation. …”
Publicado 2017
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57por Bagheri, Fereshteh, Tahvilian, Reza, Karimi, Naser, Chalabi, Maryam, Azami, Mahsa“…The objective of this research was in-vitro germination and callus induction of Onosma bulbotrichum (O. bulbotrichum) as a medicinal herb which belongs to Boraginaceae family. …”
Publicado 2018
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59“…Here, we report a method that ensures the competency of an embryogenic callus secondary culture under laboratory conditions for Agrobacterium-mediated transformation. …”
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60por Zhang, Xiaoling, Wang, Yanli, Yan, Yuanyuan, Peng, Hua, Long, Yun, Zhang, Yinchao, Jiang, Zhou, Liu, Peng, Zou, Chaoying, Peng, Huanwei, Pan, Guangtang, Shen, Yaou“…However, the differences in induction and regeneration capacity of embryonic callus (EC) among various genotypes result in genotypic dependence in genetic transformation. …”
Publicado 2019
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