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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
1
Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
1
histological analysis
1
kinetin
1
kinetina
1
organogenesis
1
somatic embryos
1
ácido diclorofenoxiacético
1
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901por Abu-Abied, Mohamad, Rogovoy (Stelmakh), Oksana, Mordehaev, Inna, Grumberg, Marina, Elbaum, Rivka, Wasteneys, Geoffrey O., Sadot, Einat“…Induction of adventitious roots (ARs) in recalcitrant plants often culminates in cell division and callus formation rather than root differentiation. …”
Publicado 2015
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902por Hurtgen, B.J., Ward, C.L., Garg, K., Pollot, B.E., Goldman, S.M., McKinley, T.O., Wenke, J.C., Corona, B.T.“…We observed that VML injury impaired tibia healing, as indicated by diminished mechanical strength and decreased mineralized bone within the fracture callus, as well as continued presence of cartilage instead of woven bone 28 days post-injury. …”
Publicado 2016
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903“…Basal media, carbohydrate source, combination of plant growth regulators and the leaf segment locations were evaluated for callus and shoot formation. The highest callus formation (10.39%) and shoot formation (4.53%) were achieved from first basal segments 3–4 days old seedlings. …”
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904“…We demonstrated an increasing frequency and severity of foot problems with an increasing age, as well as the association between the loss of protective sensation and the history of foot ulceration, foot deformity and callus formation, the history of foot ulcer or amputation and acute foot ulceration. …”
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905“…Radiological evaluation showed that callus formation of group B and C was all significantly higher than group A at 8 weeks (P=0.04 and P=0.013, respectively). …”
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906“…In order to understand the mechanisms underlying acquisition of tolerance to salinity, we recently produced callus tissues of tobacco and Medicago truncatula resistant to NaCl-induced salt stress following application of a step-up recurrent selection method. …”
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907“…Every time an embryogenic callus culture must be started from new explants, which is laborious, costly and time consuming. …”
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908“…Expression profiles showed that the ARF, Aux/IAA, GH3, and SAUR family genes were extensively involved in embryogenic competence acquisition of upland cotton callus. The Aux/IAA family genes generally showed a higher expression level in the non-embryogenic callus (NEC) of highly embryogenic cultivar CCRI24 than that of recalcitrant cultivar CCRI12, which may be conducive to initializing the embryogenic transformation. …”
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909“…Besides, a more mature callus was observed in the POF rats receiving icariin than in the untreated POF rats, while serum E(2) and uterine index were unaffected by icariin treatment. …”
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910por Khayyeri, Hanifeh, Hammerman, Malin, Turunen, Mikael J., Blomgran, Parmis, Notermans, Thomas, Guizar-Sicairos, Manuel, Eliasson, Pernilla, Aspenberg, Per, Isaksson, Hanna“…SAXS and histology revealed heterogeneous tissue maturation with more mature tissue at the peripheral region compared to the center of the callus. Thus, mechanical loading advances Achilles tendon biomechanical and collagen properties earlier compared to unloaded tendons, and the spatial variation in tissue maturation and collagen organization across the callus suggests important regional (mechano-) biological activities that require more investigation.…”
Publicado 2020
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911por De la Vega, R.E., Coenen, M.J., Müller, S.A., Nagelli, C.V., Quirk, N.P., Lopez de Padilla, C., Evans, C.H.“…Defects treated with rhBMP-2 healed with a large callus containing thin cortices and wispy trabeculae; this, too, was unaffected by FK506. …”
Publicado 2020
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912por Chang, Yuhan, Hu, Chih-Chien, Wu, Ying-Yu, Ueng, Steve W. N., Chang, Chih-Hsiang, Chen, Mei-Feng“…In particular, micro-computed tomography was used to reconstruct femoral morphology and analyze callus bone content in a femoral defect mouse model. …”
Publicado 2021
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913por Linh, Tran My, Mai, Nguyen Chi, Hoe, Pham Thi, Ngoc, Ninh Thi, Thao, Phan Thi Hong, Ban, Ninh Khac, Van, Nguyen Tuong“…The purpose of this study was to produce C. roseus callus and suspension cell biomass of good quality and quantity to improve the total alkaloids and bis-indole alkaloids. …”
Publicado 2021
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914por Appelt, Jessika, Tsitsilonis, Serafeim, Otto, Ellen, Jahn, Denise, Köhli, Paul, Baranowsky, Anke, Jiang, Shan, Fuchs, Melanie, Bucher, Christian H., Duda, Georg N., Frosch, Karl-Heinz, Keller, Johannes“…In a standardized femoral osteotomy model, global CTR-deficient mice displayed no alteration in radiologic callus parameters. Likewise, static histomorphometry demonstrated moderate impairment of callus microstructure and normal osseous bridging of osteotomy ends. …”
Publicado 2021
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915“…We achieved a 2.5-fold increase in somatic embryogenesis (up to 80%) when 50 mg L(−1) daminozide was included in the callus induction medium together with 3 mg L(−1) 2,4-D. …”
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916por Lu, Chao, Wei, Yunxiao, Meng, Zhigang, Liu, Yongming, Ali, Abid Muhammad, Liu, Qinfei, Abbas, Mubashir, Wang, Yanan, Liang, Chengzhen, Wang, Yuan, Zhang, Rui“…It was observed that EC (embryogenic callus) formation period was 33% shorter and transformation efficiency was slightly higher in the LT T0 generation than that of control. …”
Publicado 2022
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917por Kaur, Rajvinder, Donoso, Thomas, Scheske, Chelsea, Lefsrud, Mark, Singh, Jaswinder“…[Image: see text] A reproducible tissue culture protocol is required to establish an efficient genetic transformation system in highly recalcitrant pea genotypes. High-quality callus with superior regeneration ability was induced and regenerated on optimized media enriched with copper sulfate and cytokinins, 6-benzylaminopurine and indole-3-acetic acid. …”
Publicado 2022
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918por Fukase, Naomasa, Duke, Victoria R., Lin, Monica C., Stake, Ingrid K., Huard, Matthieu, Huard, Johnny, Marmor, Meir T., Maharbiz, Michel M., Ehrhart, Nicole P., Bahney, Chelsea S., Herfat, Safa T.“…Our results indicated the sensors successfully incorporated into the fracture callus and did not impede repair. Electrical impedance, resistance, and reactance increased steadily from weeks 3 to 7—corresponding to the transition from hematoma to cartilage to bone within the fracture gap—then plateaued as the bone began to consolidate. …”
Publicado 2022
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919Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of OsOr Genepor Kim, Hee Kyoung, Kim, Jin Young, Kim, Jong Hee, Go, Ji Yun, Jung, Yoo-Seob, Lee, Hyo Ju, Ahn, Mi-Jeong, Yu, Jihyeon, Bae, Sangsu, Kim, Ho Soo, Kwak, Sang-Soo, Kim, Me-Sun, Cho, Yong-Gu, Jung, Yu Jin, Kang, Kwon Kyoo“…We generated an orange-colored (OC) rice callus line by targeted mutagenesis of the orange gene (OsOr) using the CRISPR-Cas9 system. …”
Publicado 2022
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920por Martínez, María Eugenia, Jorquera, Lorena, Poirrier, Paola, Díaz, Katy, Chamy, Rolando“…The best conditions for establishing a suspension culture were achieved by using a 6-week-old callus at 4% (w/v) and 1% (w/v) of sucrose concentration. …”
Publicado 2023
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