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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
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ácido diclorofenoxiacético
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1281“…Shoots derived from the upper section were elongated, but had the shortest internodes, while those from the basal section formed the largest callus. None of the three types of explants rooted during the proliferation phase. …”
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1282por Ahmad, Mudussar A, Kuhanendran, Damayanthi, Kamande, Irvine W, Charalambides, Charalambos“…Radiographs demonstrated radiological union with good alignment and abundant callus formation. Fracture union occurred within 4 weeks, less than half the time expected for a conservatively treated tibial shaft fracture. …”
Publicado 2008
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1283“…Thus, this peptide growth factor, which promotes callus formation in culture, is proteolytically cleaved from its precursor by a specific plant subtilase encoded by a gene that is up-regulated during the process of transfering root explants to tissue culture.…”
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1284por Lee, Dong Yeon, Cho, Tae-Joon, Choi, In Ho, Chung, Chin Youb, Yoo, Won Joon, Kim, Ji Hyung, Park, Yong Koo“…Clinical, radiological, and histopathologic characteristics that distinguished this subtype from others include ossification of interosseous membrane of the forearm with radial head dislocation, hyperplastic callus formation, no evidence of type I collagenopathy and an abnormal histopathologic pattern. …”
Publicado 2006
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1285por Akritopoulos, Panagiotis, Papaioannidou, Paraskevi, Hatzokos, Ippokratis, Haritanti, Afroditi, Iosifidou, Eirini, Kotoula, Maria, Mirtsou-Fidani, Vassiliki“…In both groups fracture healing (bone union and callus formation) was evaluated with X-rays 28 and 42 days after surgery. …”
Publicado 2008
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1286“…In conclusion, best management of foot ulcers is achieved by what is taken out of the foot (pressure, callus, infection, and slough) rather than what is put on the foot (adjuvant treatment).…”
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1287por Maeda, Shintaro, Ishikawa, Hiroki, Tanigawa, Naoaki, Miyazaki, Kyosuke, Shioda, Seiji“…This suggests that dilation promotes callus formation in the bone tunnel wall and prevents the complication of bone tunnel enlargement after ligament reconstruction.…”
Publicado 2012
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1288por Bouderlique, Thibault, Henault, Emilie, Lebouvier, Angelique, Frescaline, Guilhem, Bierling, Phillipe, Rouard, Helene, Courty, José, Albanese, Patricia, Chevallier, Nathalie“…Pleiotrophin (PTN) is a growth factor present in the extracellular matrix of the growth plate during bone development and in the callus during bone healing. Bone healing is a complicated process that recapitulates endochondral bone development and involves many cell types. …”
Publicado 2014
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1289“…In contrast to somatic mammalian cells, which cannot alter their fate, plant cells can dedifferentiate to form totipotent callus cells and regenerate a whole plant, following treatment with specific phytohormones. …”
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1290“…Hence, stem cell-related factors possibly synergistically function to provide a specific niche beneficial to dedifferentiation. During callus formation in Arabidopsis petioles, cells adjacent to procambium cells (stem cell-like cells) are dedifferentiated and survive more easily than other cell types. …”
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1291por Zhu, Lu, Zheng, Chen, Liu, Ruixia, Song, Aiping, Zhang, Zhaohe, Xin, Jingjing, Jiang, Jiafu, Chen, Sumei, Zhang, Fei, Fang, Weimin, Chen, Fadi“…CmLBD1 expression also favored callus formation from A. thaliana root explants in the absence of exogenously supplied phytohormones. …”
Publicado 2016
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1292por Manivannan, Abinaya, Soundararajan, Prabhakaran, Park, Yoo Gyeong, Jeong, Byoung Ryong“…Friable calli were induced from the leaf explants cultured on a Murashige and Skoog (MS) medium containing 3.0 mg·L(−1) 6-benzyladenine (BA) in a combination with 2 mg·L(−1) 2,4-dichlorophenoxy acetic acid (2,4-D). From the callus cultures, a cell suspension culture was initiated and the cellular differentiation was investigated. …”
Publicado 2016
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1293por Corbin, Jasmine M., Hashimoto, Bryce I., Karuppanan, Kalimuthu, Kyser, Zachary R., Wu, Liying, Roberts, Brian A., Noe, Amy R., Rodriguez, Raymond L., McDonald, Karen A., Nandi, Somen“…After transformation of rice callus and screening of transformants, the cultures were scaled up from culture flask to a lab scale bioreactor. …”
Publicado 2016
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1294“…Six months after the surgery, well-regenerated bone could be recognized on radiographic evaluation and was resistant to refracture. Callus distraction is a method that aims to avoid donor site morbidity. …”
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1295por Zhang, Yi, Liang, Zhen, Zong, Yuan, Wang, Yanpeng, Liu, Jinxing, Chen, Kunling, Qiu, Jin-Long, Gao, Caixia“…Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR/Cas9 introduced as DNA or RNA. …”
Publicado 2016
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1296por Bahmankar, Moslem, Mortazavian, Seyed Mohammad Mahdi, Tohidfar, Masoud, Sadat Noori, Seyed Ahmad, Izadi Darbandi, Ali, Corrado, Giandomenico, Rao, Rosa“…Results indicated that different developmental stages of somatic embryos were simultaneously observed on a callus with embryogenic potential. The high content of catalase, ascorbate peroxidase, proline, and terpenic hydrocarbons and low content of alcoholic and phenolic compositions had a stimulatory effect on somatic embryogenesis. …”
Publicado 2017
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1297“…The patient underwent surgery with exploration of the frayed tendon caught in a voluminous bony callus (E). Conservative treatment was not possible, repair was based on resection and partial graft from the extensor carpi radialis longus after dissection via the same approach. …”
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1298“…For a successful grafting, graft union development generally involves the formation of callus and vascular bundles at the graft union. To explore the molecular mechanism of graft union development, we applied high throughput RNA sequencing to investigate the transcriptomic profiles of graft union at four timepoints (0 days, 8 days, 15 days, and 30 days) during the pecan grafting process. …”
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1299por Wong, Sarah A., Rivera, Kevin O., Miclau, Theodore, Alsberg, Eben, Marcucio, Ralph S., Bahney, Chelsea S.“…Recent studies have provided genetic evidence demonstrating that a significant portion of callus chondrocytes transform into osteoblasts that derive the new bone. …”
Publicado 2018
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1300“…For repairing rabbit radius defects in vivo, there was rapid healing in the defects treated with the 3D porous scaffold with microchannels, where the bridging by a large bony callus was observed at 12 weeks post-surgery. Based on the results, the 3D porous scaffold with microchannels was a promising candidate for bone defect repair.…”
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