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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
1
Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
1
ácido diclorofenoxiacético
1
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1381por Miyamoto, Shuichi, Otsuka, Makoto, Hasue, Fumio, Fujiyoshi, Takayuki, Kamiya, Koushirou, Kiuchi, Hitoshi, Tanaka, Tadashi, Nakamura, Junichi, Orita, Sumihisa, Ohtori, Seiji“…Because the clavicular fracture had no displacement or callus formation, conservative treatment with a clavicle band was undertaken. …”
Publicado 2019
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1382por Bächinger, David, Goosmann, Madeline M., Schuknecht, Bernhard, Nadol, Joseph B., Adams, Joe C., Huber, Alexander, Eckhard, Andreas H.“…Our findings suggest that a temporal bone fracture with a “retrolabyrinthine” course, traversing the VA, caused intraaqueductal callus bone formation and progressive blockage of the VA. …”
Publicado 2019
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1383por Wang, Xi, Matthews, Brya G, Yu, Jungeun, Novak, Sanja, Grcevic, Danka, Sanjay, Archana, Kalajzic, Ivo“…We detected broad expression of PDGF receptor beta (PDGFRβ) within the intact periosteum and healing callus during fracture repair. In vitro, periosteum‐derived progenitor cells were highly responsive to PDGF as demonstrated by increased proliferation and decreased apoptosis. …”
Publicado 2019
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1384“…Using the improved transformation system, we obtained the transgenic poplar with overexpression of β-glucuronidase (GUS) via direct organogenesis without callus induction. Furthermore, we analyzed the GUS gene in the transgenic poplars using PCR, qRT-PCR, and GUS staining. …”
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1385por Cao, Jian, Zhang, Shijian, Gupta, Anand, Du, Zhaojie, Lei, Delin, Wang, Lei, Wang, Xudong“…The rabbits were sacrificed at consolidation time of 28 days. The regenerate callus underwent radiograph examination, dual-energy X-ray absorptiometry, haematoxylin and eosin staining and histomorphometric analysis. …”
Publicado 2019
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1386“…Thus, a key gene involved in K(+) uptake under salt condition was functionally characterized using a newly established L. ruthenicum callus transformation system. The importance of K(+) regulation in L. ruthenicum under salt tolerance was highlighted.…”
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1387por Godel-Jędrychowska, Kamila, Maćkowska, Katarzyna, Kurczyńska, Ewa, Grzebelus, Ewa“…It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation. Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process. …”
Publicado 2019
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1388“…We collected embryogenic callus and somatic embryos at the globular, torpedo, and cotyledon maturation stages, as well as from plantlets, for total protein extraction. …”
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1389“…Genotypes displayed protocol-dependent, differential phenolic exudation responses, as indicated by medium browning. During the callus induction phase, genotypes prone to medium browning exhibited a response on WU medium which was either equal or greater than on LG medium. …”
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1390por Rukavtsova, E. B., Abramikhina, T. V., Shulga, N. Ya., Bykov, V. A., Bur’yanov, Ya. I.“…The level of HBs-antigen in plants carrying the HBsAg gene controlled by the dual 35S RNA cauliflower mosaic virus promoter was the same in all organs of the plant and made up to 0.06% of the total amount of soluble protein. Hairy root and callus cultures of plants carrying the HBsAg gene and expressing the HBs-antigen were obtained.…”
Publicado 2007
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1391“…Quantitative real-time polymerase chain reaction (PCR) used to detect the BMP-2 expression in the rat’s callus tissue on the fractured side. The mechanical properties of rat’s hindlimb skeleton were examined using a universal material mechanics testing machine. …”
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1392“…MATERIALS AND METHODS: The callus formation and in vitro propagation of D. pumilus (Gouan) by using a different combination of naphthalene acetic acid and benzylaminopurine were established. …”
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1393por Kirmayer, David, Grin, Ada, Gefter (Shenderovich), Julia, Friedman, Michael, Rachmilewitz, Jacob, Mosheiff, Rami, Kenett, Ron, Khoury, Amal“…The AMCA membranes were found superior to no treatment in terms of new bone formation in the defect, bone volume, callus surface area normalized to total volume, and the number of bone trabeculae, after eight weeks. …”
Publicado 2020
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1394por Dissanayake, Lakmali, Perera, Prasanthi, Attanayaka, Thilak, Heberle, Erwin, Jayawardhana, Manosha“…Its cross-pollinating nature limits the opportunity of exploitation of hybrid vigor and demands the development of homozygous lines through doubled-haploid technologies. The problems in callus-mediated embryogenesis, such as longer processing time and genetically unstable nature, can be overcome by direct embryogenesis. …”
Publicado 2020
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1395“…Generally “SNC” cultures formed one well rooted, significantly longer axillary shoot with bigger leaves than “AxB” cultures, which developed significantly more shoots and big callus at the explant base. All shoots collected from “SNC” and “AxB” cultures rooted in vivo in peat mixture and developed into similar plantlets. …”
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1396por Roseren, Flavy, Pithioux, Martine, Robert, Stéphane, Balasse, Laure, Guillet, Benjamin, Lamy, Edouard, Roffino, Sandrine“…Contrary to control group, in the early phase of consolidation, a bony bridge with lower osteoclast activity and a trend of an increase in osteoblast activity were observed in the distracted callus in the G-CSF group, whereas, at the late phase of consolidation, a significantly lower neovascularization was observed. …”
Publicado 2021
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1397por Yasuda, Tomohiro, Arai, Masayuki, Shinohara, Daichi, Samejima, Yuki, Kanzaki, Koji, Kawasaki, Keikichi“…Radiographical assessments of healing of the fracture confirmed callus formation and complete bone union 3 months and 1 year after the operation, respectively. …”
Publicado 2021
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1398“…A single dose of denosumab was administered prior to referral to endocrinology and seemed to contribute to callus formation. Denosumab was discontinued to prevent progression of the contralateral incomplete AFF. …”
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1399“…For the first time, we reported the optimum callus induction medium with a high percentage success rate of 82% from the combination of 1 mg/L indole-3-butyric acid and 5 mg/L naphthalene acetic acid. …”
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1400“…The plant-growth regulator type and concentration and Agrobacterium-mediated transformation were evaluated for their effects on duckweed callus induction, proliferation, regeneration, and gene transformation efficiency. …”
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