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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
1
ácido diclorofenoxiacético
1
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1641“…It is a self-fertile plant that can be transformed with high efficiency using Agrobacteria and callus derived from immature embryos. In addition, considerable genetic and genomic resources are becoming available for this species in the form of mapping populations, large expressed sequence tag collections, T-DNA insertion lines and, in the near future, the complete genome sequence. …”
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1642“…Over 85% presented with foot lesions that included corns and callus over the forefoot region and lesser toe deformities. …”
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1643“…All the patients had abnormal mobility and no or minimal gap at fracture site with no evidence of callus formation across the fracture site. The patients' age ranged between 24 and 68 years; 40 were men and 5 were women. …”
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1644por Nouvong, Aksone, Hoogwerf, Byron, Mohler, Emile, Davis, Brian, Tajaddini, Azita, Medenilla, Elizabeth“…The sensitivity, specificity, and positive predictive values increased to 86, 88, and 96%, respectively, when removing three false-positive osteomyelitis cases and four false-negative cases due to measurements on a callus. The results indicate that cutaneous tissue oxygenation correlates with wound healing in diabetic patients. …”
Publicado 2009
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1645por Newell, Christine A., Brown, Naomi J., Liu, Zheng, Pflug, Alexander, Gowik, Udo, Westhoff, Peter, Hibberd, Julian M.“…Co-incubation of hypocotyls or cotyledons with SLJ1006 allowed efficient transfer of DNA into C. gynandra, and media that allowed callus production and then shoot regeneration were identified. …”
Publicado 2010
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1646por Caetano-Lopes, Joana, Lopes, Ana, Rodrigues, Ana, Fernandes, Diana, Perpétuo, Inês P., Monjardino, Teresa, Lucas, Raquel, Monteiro, Jacinto, Konttinen, Yrjö T., Canhão, Helena, Fonseca, João E.“…The aim of our work was to study the sequence of gene expression events involved in inflammation and bone remodeling occurring in the early phases of callus formation in osteoporotic patients. METHODOLOGY/PRINCIPAL FINDINGS: Fifty-six patients submitted to hip replacement surgery after a low-energy hip fracture were enrolled in this study. …”
Publicado 2011
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1647por Sharathchandra, Ramaschandra G., Stander, Charmaine, Jacobson, Dan, Ndimba, Bongani, Vivier, Melané A.“…METHODOLOGY/PRINCIPAL FINDINGS: In this study we report on the proteomic evaluation of the cytosolic proteins obtained from synchronized cell suspension cultures that were established from callus lines originating from green, véraison and ripe Vitis vinifera berry explants. …”
Publicado 2011
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1648por Djordjevic, Michael A., Oakes, Marie, Wong, Chui E., Singh, Mohan, Bhalla, Prem, Kusumawati, Lucia, Imin, Nijat“…Using in vitro root growth assays and an in vitro root and callus formation assay it is shown that CLE36 peptides of different lengths possess differential biological activities. …”
Publicado 2011
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1649“…In this present investigation, we report, biomedical potential of silver nanopaticles synthesized from calli extract of Citrullus colocynthis on Human epidermoid larynx carcinoma (HEp -2) cell line. METHODS: The callus extract react with silver nitrate solution confirmed silver nanoparticles synthesis through the steady change of greenish colour to reddish brown and characterized by using FT-IR, AFM. …”
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1650por Harding, Anna Kajsa, W-Dahl, Annette, Geijer, Mats, Toksvig-Larsen, Sören, Tägil, Magnus“…BACKGROUND: Bisphosphonates increase the callus size and strength in animal fracture studies. …”
Publicado 2011
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1651por Naik, Amish A, Xie, Chao, Zuscik, Michael J, Kingsley, Paul, Schwarz, Edward M, Awad, Hani, Guldberg, Robert, Drissi, Hicham, Puzas, J Edward, Boyce, Brendan, Zhang, Xinping, O'Keefe, Regis J“…Aging was associated with a decreased rate of chondrogenesis, decreased bone formation, reduced callus vascularization, delayed remodeling, and altered expression of genes involved in repair and remodeling. …”
Publicado 2009
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1652por Murata, Koichi, Kitaori, Toshiyuki, Oishi, Shinya, Watanabe, Naoki, Yoshitomi, Hiroyuki, Tanida, Shimei, Ishikawa, Masahiro, Kasahara, Takashi, Shibuya, Hideyuki, Fujii, Nobutaka, Nagasawa, Takashi, Nakamura, Takashi, Ito, Hiromu“…We demonstrated that SDF-1 protein was expressed at pre-hypertrophic and hypertrophic chondrocytes in the newly formed endochondral callus of rib fracture as well as in the growth plate of normal mouse tibia by immunohistochemical analysis. …”
Publicado 2012
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1653por Xia, Tongmei, Xiao, Dong, Liu, Dong, Chai, Wenting, Gong, Qingqiu, Wang, Ning Ning“…Constitutive expression of GmATG8c in soybean callus cells not only enhanced nitrogen starvation tolerance of the cells but accelerated the growth of the calli. …”
Publicado 2012
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1654por Carlier, Aurélie, Geris, Liesbet, Bentley, Katie, Carmeliet, Geert, Carmeliet, Peter, Van Oosterwyck, Hans“…The healing of a fracture depends largely on the development of a new blood vessel network (angiogenesis) in the callus. During angiogenesis tip cells lead the developing sprout in response to extracellular signals, amongst which vascular endothelial growth factor (VEGF) is critical. …”
Publicado 2012
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1655“…In addition, we have included cell wall analysis of embryonic callus used for genetic transformations. Composition of cell walls based on components lignin, hydroxycinnamates, uronosyls, neutral sugars, and protein suggests that Brachypodium distachyon is similar chemically to agriculturally important forage grasses. …”
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1656“…INTRODUCTION: Radial bone loss associated with gross manus valgus deformity can be managed by open reduction internal fixation using intervening strut bone graft, callus distraction using ring or monoaxial fixator, and achieving union by distraction histogenesis. …”
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1657“…Shahi × C. cauliflora produced maximum callus, as well as somatic embryos when cultured on half-strength MS medium containing 5 mg/L 2,4-D, 100 mg/L glutamine, 100 mg/L casein hydrolysate and 60 g/L sucrose. …”
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1658“…In this study, small RNAs and their targets were identified during cotton somatic embryogenesis (SE) through high-throughput small RNA and degradome sequencing, comparing seedling hypocotyl and embryogenic callus (EC) of G. hirsutum YZ1. A total of 36 known miRNA families were found to be differentially expressed, of which 19 miRNA families were represented by 29 precursors. …”
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1659“…The one-leg plates behave as the fulcrum of the leverage system and make the wedge tip the zone of tension and thus have been reported to negatively affect the callus formation. CONCLUSIONS: The choice of the HTO plates involved the trade-off between surgical convenience, construct stability, and stress-shielding effect. …”
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1660“…RESULTS: Hairy roots produced five-fold higher yields of justicidin B (7.78mg/g DW) compared to callus. A rapidly growing root line was selected for cultivation in 2-L stirred tank bioreactor. …”
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