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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
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ácido diclorofenoxiacético
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1721“…Moreover, NbSKη-RNAi, 35S::NbCycD1;1 transgenic N. benthamiana plants have the similar phenotype as 35S::C4 transgenic N. benthamiana plants on callus-like tissue formation resulted from abnormal cell division induction. …”
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1722por Palacio, Evandro Pereira, Pereira, Gilberto José Cação, Silvares, Paulo Roberto de Almeida, Stasi, Gabriel Guimarães Di, Staut, Caio de Andrade, Sardenberg, Trajano“…The humerus from both groups had similar bone callus area (p = 0.66). In both groups, there were no statistical differences related to inflammatory cells (p = 0.4), osteoblasts (p = 0.2), and osteoclasts (p = 0.2). …”
Publicado 2017
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1723por Mathavan, Neashan, Turunen, Mikael J., Guizar-Sicairos, Manuel, Bech, Martin, Schaff, Florian, Tägil, Magnus, Isaksson, Hanna“…And how do pharmacological treatments, such as bone morphogenetic protein 7 (BMP-7) alone or synergistically combined with zoledronate (ZA), alter callus composition and nano-structure in such environments? …”
Publicado 2018
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1724por Bulgakov, Victor P., Vereshchagina, Yulia V., Bulgakov, Dmitry V., Veremeichik, Galina N., Shkryl, Yuri N.“…In the present work, we performed a proteomic analysis of Arabidopsis thaliana rolB-expressing callus line AtB-2, which represents a line with moderate expression of the oncogene. …”
Publicado 2018
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1725por Kasnavieh, Seyed Mohammad Hosseini, Sadeghi, Seyed Mohammad Hasan, Khameneh, Seyed Mehdi Hosseini, Khodadoost, Mahmood, Bazrafshan, Azam, Kamalinejad, Mohammad, Jaladat, Amir Mohammad, Jafari, Shirin, Yasinzadeh, Mohammad Reza, Gachkar, Latif“…Some light meals like chicken soup are recommended for the early days of fracture and high-nutrient and dense foods such as goat’s or sheep’s head and nuts are advised in following days for fracture healing acceleration and callus formation. Several recommendations are also provided for pacing the healing process. …”
Publicado 2017
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1726“…In the rat femoral fracture model, the local administration of SEC2 accelerated fracture healing by increasing fracture callus volumes, bone volume over total volume (BV/TV), and biomechanical recovery. …”
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1727“…The newly established dynamic model can simulate the healing process under different mechanical environments and has the potential to extend to the multiscale healing model, which is essential for reducing the animal experiments and helping to characterise the complex dynamic interaction between tissue differentiations within the callus region.…”
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1728por Van Eck, Joyce“…The tissue culture-based method utilizes A. tumefaciens infection of mature seed-derived callus with subsequent recovery of stable transgenic lines. …”
Publicado 2018
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1729“…By strategically exploiting the transcriptomes of chiloglottone 1-producing Chiloglottis trapeziformis at distinct floral tissues and at key floral developmental stages, we identified two key transcriptional trends linked to the stage- and tissue-dependent distribution profiles of chiloglottone in the flower: (i) developmental upregulation of fatty acid biosynthesis and β-oxidation genes such as KETOACYL-ACP SYNTHASE, FATTY ACYL-ACP THIOESTERASE, and ACYL-COA OXIDASE during the transition from young to mature buds and flowers and (ii) the tissue-specific induction of fatty acid pathway genes in the callus (the insectiform odor-producing structure on the labellum of the flower) compared to the labellum remains (non-odor-producing) regardless of development stage of the flower. …”
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1730por Rong, Xiao Fei, Sang, Ya Lin, Wang, Liang, Meng, Wen Jing, Zou, Chun Hao, Dong, Yu Xiu, Bie, Xiao Min, Cheng, Zhi Juan, Zhang, Xian Sheng“…For type II, carpels were generated directly from callus. The transcript level of AGAMOUS (AG), the carpel identity gene, was up-regulated during carpel induction. …”
Publicado 2018
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1731“…Patients with infection also had a significantly longer ulcer duration (125.6 vs. 54.2 days), larger ulcer size (2.47 vs. 1.99 cm(2)), a higher rate of tissue necrosis in the ulcer bed (55.6% vs. 20.0%), a lower rate of callus at the edge (27.8% vs. 70.0%), and a higher moisture level than did patients without infection. …”
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1732por Singh, Amritpal, Jiong Hao, Jonathan Tan, Wei, Desmond Tan, Liang, Chua Wei, Murphy, Diarmuid, Thambiah, Joseph, Han, Chee Yu“…Time to fracture union was based on radiological evidence of callus bridging at least three cortices. Information on infection rates and the presence of microbes were evaluated. …”
Publicado 2018
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1733“…Appropriately timed stimulation of chondrogenesis and osteogenesis in the fracture callus may be a more effective approach for preventing or treating DNFs compared with stimulation of osteogenesis alone. © 2016 The Authors. …”
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1734“…Psoralen + rhBMP-2 exerted more beneficial effects on callus consolidation and biomechanical strength. In addition, increased bone-specific alkaline phosphatase levels and decreased C-terminal telopeptide of type-1 collagen were observed in the Psoralen + rhBMP-2 group. …”
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1735“…Materials and methods: Callus of E. longifolia was cultured in MS medium supplemented with 0.8% agar, 30/L sucrose, 1.25 mg/L NAA and 1 mg/L KIN for biomass production. …”
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1736“…Bone transport started 14 days postoperatively at 1 mm/day and was adjusted according to the callus-to-diameter ratio. The bone transport time, bone graft fusion, relapse, and metastasis were recorded. …”
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1737por Yeh, Yu-Cheng, Yang, Cheng-Chun, Tai, Ching-Lung, Tsai, Tsung-Ting, Lai, Po-Liang, Fu, Tsai-Sheng, Niu, Chi-Chien, Chen, Lih-Huei, Chen, Wen-Jer“…RESULTS: At postoperative 12 weeks, callus formation was observed at the fusion sites in all rats, with the mean radiological evaluations of 2.75/3 according to the Bransford classification. …”
Publicado 2017
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1738por Lee, Kyoung-Mi, Park, Kwang Hwan, Hwang, Ji Suk, Lee, Moses, Yoon, Dong Suk, Ryu, Hyun Aae, Jung, Ho Sun, Park, Ki Won, Kim, Jihyun, Park, Sahng Wook, Kim, Sung-Hwan, Chun, Yong-Min, Choi, Woo Jin, Lee, Jin Woo“…In a murine fracture model, STAT5A deletion was found to significantly enhance bone remodeling by stimulating the formation of a fracture callus. Our findings indicate that STAT5A inhibition enhances bone formation by promoting osteogenesis of BMSCs.…”
Publicado 2018
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1739por Hesami, Mohsen, Daneshvar, Mohammad Hosein, Yoosefzadeh-Najafabadi, Mohsen, Alizadeh, Milad“…According to our results, the maximum callus induction frequency (100%) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) plus 0.05 mg/l 6-benzylaminopurine (BAP) from petiole segments. …”
Publicado 2018
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1740“…Inclusion of 8.80 μM BA into MS supplemented medium triggered a high frequency of regeneration response from split node explants with a maximum number of shoots (12.0 ± 1.15) and the highest shoot length (3.0 ± 0.73) was obtained directly (without an intervening callus phase) within 4 weeks of culture. Further shoot elongation was achieved on medium containing 4.40 μM BA. …”
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