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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
1
ácido diclorofenoxiacético
1
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1861por Dey, Mohitosh, Bakshi, Souvika, Galiba, Gabor, Sahoo, Lingaraj, Panda, Sanjib Kumar“…Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage. Best shoot proliferation response (10.3 shoots per explant) was recorded when shoot apices were cultured on media supplemented with 4 mg/l TDZ. …”
Publicado 2012
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1862por Zhai, Jun-Wen, Zhang, Guo-Qiang, Chen, Li-Jun, Xiao, Xin-Ju, Liu, Ke-Wei, Tsai, Wen-Chieh, Hsiao, Yu-Yun, Tian, Huai-Zhen, Zhu, Jia-Qiang, Wang, Mei-Na, Wang, Fa-Guo, Xing, Fu-Wu, Liu, Zhong-Jian“…METHODOLOGY/PRINCIPAL FINDINGS: A new orchid possesses a labellum with a large Y-shaped callus and two sacs at the base, and cylindrical, fleshy seeds, which make it distinct from all known orchid genera. …”
Publicado 2013
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1863por Neves, Paulo César Fagundes, de Campos Vieira Abib, Simone, Neves, Rogério Fagundes, Pircchio, Oronzo, Saad, Karen Ruggeri, Saad, Paulo Fernandes, Simões, Ricardo Santos, Moreira, Marcia Bento, de Souza Laurino, Cristiano Frota“…After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red. …”
Publicado 2013
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1864por Bonnet, Nicolas, Gineyts, Evelyne, Ammann, Patrick, Conway, Simon J., Garnero, Patrick, Ferrari, Serge“…Bone damage removal and callus formation in response to fatigue loading are essential to prevent fractures. …”
Publicado 2013
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1865por Konieczny, Robert, Banaś, Agnieszka K., Surówka, Ewa, Michalec, Żaneta, Miszalski, Zbigniew, Libik-Konieczny, Marta“…Explants became competent to respond to the rhizogenic action of auxin on day 3 of culture, when hydrogen peroxide content in cultured tissue was the highest. l-Ascorbic acid added to the medium at 5 μM lowered the H(2)O(2) level, inhibited rhizogenesis and induced non-regenerative callus, suggesting that certain level of H(2)O(2) is required to promote root initiation. …”
Publicado 2013
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1866por Fu, Yin-Chih, Lin, Chih-Chun, Chang, Je-Ken, Chen, Chung-Hwan, Tai, I-Chun, Wang, Gwo-Jaw, Ho, Mei-Ling“…Results from the in vivo study also demonstrated that both the 7-day and 25-day treatments of SPEMF increased callus formation around the necrotic bone and also increased new vessel formation and osteocyte numbers in the grafted necrotic bone at the 2(nd) and 4(th) weeks after surgery. …”
Publicado 2014
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1867“…Grand Naine) displayed ‘Brownian motion’-reminiscent abundant motile micro-particles under bright-field and phase-contrast (×1000), which appeared similar in size and motility to the pure cultures of endophytes previously isolated from banana. Observations on callus, embryonic cells and protoplasts with intact cell wall/plasma membrane confirmed their cytoplasmic nature. …”
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1868“…Seedlings cultured for 145 days developed microshoots or callus growth in the axils of older leaves and exhibited necrosis of original seedling roots and leaves. …”
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1869por Du, Zhaojie, Wang, Lei, Zhao, Yinghua, Cao, Jian, Wang, Tao, Liu, Peng, Zhang, Yabo, Yang, Xinjie, Cheng, Xiaobing, Liu, Baolin, Lei, Delin“…In this study, a rat model of mandibular distraction osteogenesis with transection of cervical sympathetic trunk was used to demonstrate that sympathetic denervation can deplete norepinephrine (NE) in distraction-induced bone callus, down-regulate β3-adrenergic receptor (adrb3) in bone marrow mesenchymal stem cells (MSCs), and promote MSC migration from perivascular regions to bone-forming units. …”
Publicado 2014
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1870“…Cox-2 gene therapy could promote bony union through hematopoietic precursor proliferation during endochondral bone repair and thereby enhances subsequently fracture callus remodeling that leads to bony union of the fracture gap.…”
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1871por Woodbury, M. Gail, Sibbald, R. Gary, Ostrow, Brian, Persaud, Reneeka, Lowe, Julia M.“…Reliability scores of the screening tool assessment items were: ‘monofilament test’ 0.98; ‘active ulcer’ 0.97; ‘previous amputation’ 0.97; ‘previous ulcer’ 0.97; ‘fixed ankle’ 0.91; ‘deformity’ 0.87; ‘callus’ 0.87; ‘absent pulses’ 0.87; ‘fixed toe’ 0.80; ‘blisters’ 0.77; ‘ingrown nail’ 0.72; and ‘fissures’ 0.55. …”
Publicado 2015
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1872por Li, Peirong, Zhang, Shujiang, Zhang, Shifan, Li, Fei, Zhang, Hui, Cheng, Feng, Wu, Jian, Wang, Xiaowu, Sun, Rifei“…An expression analysis of the carotenoid biosynthetic genes suggested that their expression levels differed in root, stem, leaf, flower, callus, and silique tissues. Additionally, the paralogs of each carotenoid biosynthetic gene, which were generated from the WGT in B. rapa, showed significantly different expression levels among tissues, suggesting differentiated functions for these multi-copy genes in the carotenoid pathway. …”
Publicado 2015
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1873por Zhang, Hongyu, Luo, Ming, Day, Robert C., Talbot, Mark J., Ivanova, Aneta, Ashton, Anthony R., Chaudhury, Abed M., Macknight, Richard C., Hrmova, Maria, Koltunow, Anna M.“…The mutant embryos differentiated partially on rescue medium with some forming callus. HES (At1g08845) encodes a mitochondrially targeted member of a highly diverged L18 ribosomal protein family. …”
Publicado 2015
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1874por Wang, Wenlei, Li, Huanqin, Lin, Xiangzhi, Yang, Shanjun, Wang, Zhaokai, Fang, Baishan“…Transcriptome analysis and the identification of potential unigenes involved in the formation and regeneration of callus or direct induction of ABs are essential. Herein, the CK, EWAB and NPA G. lichenoides transcriptomes were analyzed using the Illumina sequencing platform in first time. …”
Publicado 2015
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1875“…In this study, CRISPR/Cas reagents expressing one of two single-guide RNA (sgRNA) targeting the potato ACETOLACTATE SYNTHASE1 (StALS1) gene were tested for inducing targeted mutations in callus and stable events of diploid and tetraploid potato using Agrobacterium-mediated transformation with either a conventional T-DNA or a modified geminivirus T-DNA. …”
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1876por Rosati, Marco, Andreani, Lorenzo, Poggetti, Andrea, Zampa, Virna, Parchi, Paolo, Lisanti, Michele“…The causes of this complication were due to the presence of an exuberant callus, to technical surgery errors or to vascular lesions. …”
Publicado 2013
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1877“…Axenic gametes of the marine green macroalga Ulva mutabilis Føyn (Ria Formosa, locus typicus) exhibit abnormal development into slow-growing callus-like colonies with aberrant cell walls. Under laboratory conditions, it was previously demonstrated that all defects in growth and thallus development can be completely abolished when axenic gametes are inoculated with a combination of two specific bacterial strains originally identified as Roseobacter sp. strain MS2 and Cytophaga sp. strain MS6. …”
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1878por Deng, Tian-ge, Liu, Chang-kui, Liu, Ping, Zhang, Lin-lin, Wu, Li-geng, Zhou, Hong-zhi, Ding, Yu-xiang, Hu, Kai-jin“…Spiral CT and micro-CT demonstrated that, compared to the experimental group, the control group showed calcified callus formation in the joint space and roughened articular surfaces after new bone formation, which protruded into the joint space. …”
Publicado 2016
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1879por Lin, Jian-Ping, Shi, Zhan-Jun, Shen, Ning-Jiang, Wang, Jian, Li, Zao-Min, Xiao, Jun“…Diagnosis and treatment of nonunion are mainly performed based on the interpretation of clinico-radiographic findings, which depend on the clinician's experience and the degree of bone callus formation during the fracture-healing process. …”
Publicado 2016
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1880por Marasco, Silvana, Quayle, Margaret, Summerhayes, Robyn, Šutalo, Ilija D., Liovic, Petar“…Follow up at 3 and 6 months was performed with three dimensional computed tomography scanning to assess for bone alignment, callus formation and healing, residual deformity, hardware failure or cut through. …”
Publicado 2016
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