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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
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ácido diclorofenoxiacético
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1941por Pecin, Marko, Stokovic, Nikola, Ivanjko, Natalia, Smajlovic, Ana, Kreszinger, Mario, Capak, Hrvoje, Vrbanac, Zoran, Oppermann, Hermann, Maticic, Drazen, Vukicevic, Slobodan“…The healing was then followed for the next 16 months. The callus formation was observed on x-ray images 2 months following ABGS implantation. …”
Publicado 2021
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1942por McGowan, Lucy M, Kague, Erika, Vorster, Alistair, Newham, Elis, Cross, Stephen, Hammond, Chrissy L“…Surprisingly, there was no evidence that the recruitment of innate immune cells to fractures or soft callus formation was altered in wnt16 mutants. However, osteoblast recruitment was significantly delayed in wnt16 mutants postfracture, coinciding with precocious activation of the canonical Wnt signaling pathway. …”
Publicado 2021
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1943por Liu, Yuelin, Luo, Weifeng, Linghu, Qianyan, Abe, Fumitaka, Hisano, Hiroshi, Sato, Kazuhiro, Kamiya, Yoko, Kawaura, Kanako, Onishi, Kazumitsu, Endo, Masaki, Toki, Seiichi, Hamada, Haruyasu, Nagira, Yozo, Taoka, Naoaki, Imai, Ryozo“…Taken together, this report demonstrates that the in planta genome editing method through SAM bombardment can be applicable to elite wheat varieties that are otherwise reluctant to callus culture.…”
Publicado 2021
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1944por Harper, Nancy S., Eddleman, Sonja, Shukla, Khushbu, Narcise, Maria Veronica, Padhye, Laura J., Peterson, Loralie J., Murati, Michael A., George, Caroline L. S.“…Furthermore, skull fractures do not offer an indication of the stage of healing on radiologic studies because they do not heal with callus formation as seen with long bone fractures. …”
Publicado 2021
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1945“…The presence of bridging callus formation on anterior-posterior and lateral radiographs was evaluated for a union. …”
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1946“…After the first stage using polymethylmethacrylate (PMMA) beads, X-rays showed that new callus developed after 2 to 4 months, and the defects were repaired completely by 5 months. …”
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1947por Tamir, Eran, Tamar, Michael, Ayalon, Moshe, Koren, Shlomit, Shohat, Noam, Finestone, Aharon S.“…There was 1 recurrence (under a hypertrophic callus of the osteotomy) during a median follow-up of 18.3 months (range, 12.2-27). …”
Publicado 2020
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1948por Li, Xueyuan, Sandgrind, Sjur, Moss, Oliver, Guan, Rui, Ivarson, Emelie, Wang, Eu Sheng, Kanagarajan, Selvaraju, Zhu, Li-Hua“…The results showed that relatively high concentrations of NAA (0.5 mg l(−1)) and 2,4-D (0.5 mg l(−1)) in the MI medium were essential for protoplasts to form cell walls and maintain cell divisions, and thereafter auxin should be reduced for callus formation and shoot induction. For shoot regeneration, relatively high concentrations of cytokinin were required, and among all the combinations tested, 2.2 mg l(−1) TDZ in combination with auxin 0.5 mg l(−1) NAA gave the best result with up to 45% shoot regeneration. …”
Publicado 2021
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1949“…Following positive–negative selection to exclude transgenic cells randomly transformed with T‐DNA, piggyBac‐mediated transgenesis from the extrachromosomal dsT‐DNA was successful in ca. 1% of transgenic callus lines. After temporary expression of CRISPR/Cas9 within piggyBac, we confirmed, in a proof‐of‐concept experiment, that piggyBac could be excised precisely from the genome via the stably transformed transposase PBase. …”
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1950por Kim, Jae-Hyun, Kim, Minsun, Hong, SooYeon, Kim, Eun-Young, Lee, Hyangsook, Jung, Hyuk-Sang, Sohn, Youngjoo“…In the femur fracture model, micro-CT analysis showed that albiflorin played a positive role in the formation of callus in the early stage of fracture recovery, and histological examination proved to induce the expression of osteogenic genes in femur tissue. …”
Publicado 2021
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1951por Saul, Dominik, Hohl, Friederike Eva, Franz, Max Konrad, Meyer, Ilka, Taudien, Stefan, Roch, Paul Jonathan, Sehmisch, Stephan, Komrakova, Marina“…Zileuton showed a beneficial effect on trabecular vertebra, while the femur was negatively affected. Callus formation was enhanced by all treatments; however, its density and biomechanical properties were unaltered. …”
Publicado 2021
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1952por Gorter, E.A., Reinders, C.R., Krijnen, P., Appelman-Dijkstra, N.M., Schipper, I.B.“…RESULTS: In animal fracture models osteoporosis was associated with decreased callus formation and bone growth, bone mineral density, biomechanical strength and delayed cellular and differentiation processes during fracture healing. …”
Publicado 2021
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1953por Munasinghe, Sachithri, Somaratne, Seneviratnege, Weerakoon, Shyama, Ranasinghe, Chandani“…Phaeocremonium parasitica, Trichoderma viride and Lasidiplodia theobromae were more effective in the production of sesquiterpenes in G. walla callus and cell suspension by biological elicitation. …”
Publicado 2021
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1954por Wong, Sarah A., Hu, Diane P., Slocum, Joshua, Lam, Charles, Nguyen, Michael, Miclau, Theodore, Marcucio, Ralph S., Bahney, Chelsea S.“…Lineage tracing with Aggrecan‐Cre(ERT2)::Ai9 tdTomato mice demonstrated that mandibular callus chondrocytes also directly contribute to the formation of new bone. …”
Publicado 2020
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1955“…Using a mouse tibial bone fracture model in vivo, we show that application of a low intensity constant EM field source enhanced fracture repair via improved biomechanical properties and increased callus bone mineralization. Overall, this study provides supporting evidence that EM field therapy promotes bone fracture repair through mitochondrial OxPhos activation.…”
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1956por Sadeghifar, Amirreza, Sheibani, Mohamad, Joukar, Siyavash, Dabiri, Shahriar, Alavi, Samanehsadat, Azari, Omid, Vosoghi, Darioush, Zeynali, Yas, Zeynali, Yasman, Shahraki, Mohamad, Torghabe, Amirhesam, Rostamzadeh, Farzaneh, Nasri, Alireza“…The density of VEGF and CD34 showed that the angiogenesis processes were different in the bone fracture area and callus tissue in the Fx +WTS subgroups. The serum levels of VEGF, TGF-β, and IGF-1 were significantly lower in the Fx +WTS42 group, and PTH in the Fx +WTS28 group was higher than that in the other groups. …”
Publicado 2021
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1957por Dong, Shujie, Qin, Yinping Lucy, Vakulskas, Christopher A., Collingwood, Michael A., Marand, Mariam, Rigoulot, Stephen, Zhu, Ling, Jiang, Yaping, Gu, Weining, Fan, Chunyang, Mangum, Anna, Chen, Zhongying, Yarnall, Michele, Zhong, Heng, Elumalai, Sivamani, Shi, Liang, Que, Qiudeng“…Strikingly, the gene editing rate was increased to 60% on average and up to 100% in some experiments when the Cas12a RNP was co-delivered with a PMI selectable marker gene cassette and the induced callus cultures were selected with mannose. We also show that use of higher activity Cas12a mutants resulted in improved editing efficiency in more recalcitrant target sequence. …”
Publicado 2021
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1958por Sasaki, Gen, Watanabe, Yoshinobu, Yasui, Youichi, Matsui, Kentaro, Kawano, Hirotaka, Miyamoto, Wataru“…Routine postoperative radiographs confirmed a sequential healing process and callus formation in three out of four cortices 3 years after surgery. …”
Publicado 2021
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1959por Jiang, Weidong, Zhu, Peiqi, Huang, Fangfang, Zhao, Zhenchen, Zhang, Tao, An, Xiaoning, Liao, Fengchun, Guo, Lina, Liu, Yan, Zhou, Nuo, Huang, Xuanping“…In vivo, METTL3-overexpressing EPCs were additionally transplanted into the DO callus, significantly enhancing bone regeneration as evidenced by improved radiological and histological manifestations in a canine mandibular DO model after consolidation over a 4-week period. …”
Publicado 2021
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1960por Evola, Francesco Roberto, Di Fede, Giovanni Francesco, Bonanno, Santo, Evola, Giuseppe, Cucuzza, Maria Elena“…Healing of the fracture occurred within six weeks after surgery, as indicated by the presence of calluses on at least three cortices on standard radiographs. …”
Publicado 2021
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