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2, 4-D participó en la inducción de embriones globulares en semillas maduras y explantes de plántulas
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2, 4-D was involved in inducing globular embryo in mature seeds and seedlings explants
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Biotecnología vegetal
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Cultivo de células vegetales
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Cultivo de tejidos vegetales
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Kinetin (0.5 mg∙L -1 ) induced all the somatic embryo phases in green compact callus explant
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La kinetina (0.5 mg∙L -1 ) indujo todas las fases del embrión somático en explantes de callos verdes compactos. verde compacto
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Los explantes de callo compacto fueron los más eficientes para inducir 19,2 embriones somáticos por explante. por explante
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Other endogenous phytohormones likely contributed to the torpedo phase that did not germinate
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Otras fitohormonas endógenas contribuyeron probablemente a la fase de torpedo que no germinó
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Plantas transgénicas
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Se evaluaron los reguladores del crecimiento para la inducción de embriones somáticos de Echinocactus parryi
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The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant
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The growth regulators for the induction of somatic embryos of Echinocactus parryi were evaluated
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análisis histológico
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cultivo de tejidos
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embriogénesis
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growth regulators
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histological analysis
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kinetin
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kinetina
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organogenesis
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somatic embryos
1
ácido diclorofenoxiacético
1
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1961por Steppe, Lena, Krüger, Benjamin Thilo, Tschaffon, Miriam Eva Angelica, Fischer, Verena, Tuckermann, Jan, Ignatius, Anita, Haffner-Luntzer, Melanie“…However, it remains to be elucidated which cell types in the fracture callus are targeted by LMHFV during bone healing. …”
Publicado 2021
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1962“…Hematoxylin and eosin staining results showed that the recovery effect of trabecular bone and callus in the cases of group A was better than the other two groups after intragastric administration. …”
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1963“…CONCLUSION: In the surgical treatment of hallux valgus, more careful surgical technique is required for patients with second plantar callus on the metatarsophalangeal joint before surgery, and efforts are needed to reduce foot width through surgical treatment.…”
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1964por Jaramillo, Angélica M., Sierra, Santiago, Chavarriaga-Aguirre, Paul, Castillo, Diana Katherine, Gkanogiannis, Anestis, López-Lavalle, Luis Augusto Becerra, Arciniegas, Juan Pablo, Sun, Tianhu, Li, Li, Welsch, Ralf, Boy, Erick, Álvarez, Daniel“…Furthermore, we evaluated whether expression of one of the variants, MeOR_X1, affected carotenoid accumulation in cassava Friable Embryogenic Callus (FEC). Overexpression of maize PSY1 alone resulted in carotenoids accumulation and induced crystal formation. …”
Publicado 2022
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1965por Liu, Sitong, Wang, Xinfeng, Ding, Ning, Liu, Yutong, Li, Ning, Ma, Yiqiao, Zhao, Jing, Wang, Zhenhui, Li, Xiaomeng, Fu, Xueqi, Li, Linfeng“…We identified abundant single nucleotide polymorphisms (SNPs) that have accumulated in cultured ginseng callus and are retained in the process of plant regeneration. …”
Publicado 2021
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1966por Setiawati, Rosy, Lay, Eveline Stephanie, Testini, Valentina, Rahardjo, Paulus, Edward, Mouli, Mustokoweni, Sjahjenny, Guglielmi, Giuseppe“…After following the chemotherapy as the treatment of choice, the radiograph and MRI evaluation were done and showed reduction of the mass size with union of the destructed part with the formation of callus. The advance MRI revealed reduction of the overall mass and the composition of the viable area compared to previous study. …”
Publicado 2021
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1967por Xu, Jiajia, Wang, Yiyun, Li, Zhu, Tian, Ye, Li, Zhao, Lu, Amy, Hsu, Ching-Yun, Negri, Stefano, Tang, Cammy, Tower, Robert J., Morris, Carol, James, Aaron W.“…The diphtheria toxin-mediated ablation of PDGFRα reporter(+) cells led to deficits in cortical bone formation during homeostasis and a diminutive hard callus during fracture repair. After ossicle transplantation, both mouse PDGFRα reporter(+) periosteal cells and human Pdgfrα(+) periosteal progenitors expand, ossify, and recruit marrow to a greater extent than their counterpart periosteal cells, whereas PDGFRα reporter(−) periosteal cells exhibit a predisposition to chondrogenesis in vitro. …”
Publicado 2022
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1968“…Here, we successfully produced overexpressing transgenic African rice with higher CPD photolyase activity by modifying media conditions for callus induction and regeneration using the parental line (PL), UVB-sensitive African rice TOG12380 (O. glaberrima). …”
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1969por Leiblein, Maximilian, Winkenbach, Andreas, Koch, Elias, Schaible, Alexander, Büchner, Hubert, Marzi, Ingo, Henrich, Dirk, Nau, Christoph“…Small granules lead to significant increase in callus formation in the vicinity to the membrane. …”
Publicado 2020
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1970por Stegen, Steve, Loopmans, Shauni, Stockmans, Ingrid, Moermans, Karen, Carmeliet, Peter, Carmeliet, Geert“…A similar metabolic adaptability between serine uptake and de novo synthesis is observed in the cartilage callus during fracture repair. Together, the results of this study reveal a critical role for PHGDH-dependent serine synthesis in maintaining intracellular serine levels under physiological and serine-limited conditions, as adequate serine levels are necessary to support chondrocyte proliferation during endochondral ossification.…”
Publicado 2022
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1971“…Fifteen randomly selected putative transgenic plants of Summer and Blackwell, representing independent callus events, were confirmed as transgenic by the presence of the transgene, ZmAls, and the absence of AtuFtsZ, a chromosomal gene specific to the Agrobacterium strain LBA4404 using polymerase chain reaction. …”
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1972por Hassani, Seyedeh Batool, Trontin, Jean-François, Raschke, Juliane, Zoglauer, Kurt, Rupps, Andrea“…In addition, transgenic somatic seedlings of P. pinaster showed non-embryogenic callus formation in the region of roots and subsequently inhibited root growth. …”
Publicado 2022
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1973por Che, Ping, Wu, Emily, Simon, Marissa K., Anand, Ajith, Lowe, Keith, Gao, Huirong, Sigmund, Amy L., Yang, Meizhu, Albertsen, Marc C., Gordon-Kamm, William, Jones, Todd J.“…Using Agrobacterium-mediated transformation, we have demonstrated Wus2-induced direct somatic embryo formation and regeneration, bypassing genotype-dependent callus formation and significantly shortening the tissue culture cycle time. …”
Publicado 2022
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1974por Miljanić, Vanja, Rusjan, Denis, Škvarč, Andreja, Chatelet, Philippe, Štajner, Nataša“…A half-strength MS medium including vitamins supplemented with 30 g/L of sucrose and solidified with 8 g/L of agar, without plant growth regulators, was used for the growth and root development of micrografts and the subsequently micropropagated plants; no callus formation, hyperhydricity, or necrosis of shoot tips was observed. …”
Publicado 2022
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1975por Wang, Yu, Zhang, He, Ri, Hyok Chol, An, Zeyu, Wang, Xin, Zhou, Jia-Nan, Zheng, Dongran, Wu, Hao, Wang, Pengchao, Yang, Jianfei, Liu, Ding-Kun, Zhang, Diyang, Tsai, Wen-Chieh, Xue, Zheyong, Xu, Zhichao, Zhang, Peng, Liu, Zhong-Jian, Shen, Hailong, Li, Yuhua“…The loss of 12 exons in the dammarenediol synthase (DDS)-encoding gene in A. elata after divergence from Panax might have caused the lack of dammarane-type saponin production, and a complementation assay shows that overexpression of the PgDDS gene from Panax ginseng in callus of A. elata recovers the accumulation of dammarane-type saponins. …”
Publicado 2022
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1976por Don, Siti Madihah Mohd, Hamid, Nur Maziyyah Abdul, Taha, Hussein, Sukri, Rahayu Sukmaria, Metali, Faizah“…For micropropagation, callus induction was not promising and could only be observed at specific concentrations of both IBA and KN, with H. imperialis appearing to be more responsive towards these hormones in comparison to H. coronaria. …”
Publicado 2021
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1977por Mao, Jiangping, Ma, Doudou, Niu, Chundong, Ma, Xiaolong, Li, Ke, Tahir, Muhammad Mobeen, Chen, Shiyue, Liu, Xiuxiu, Zhang, Dong“…The expression of genes related to the CK signaling pathway and shoot development was higher in GL-3 than in MdWOX11-OE transgenic plants during the callus and AS emergence stages. However, the expression of MdCKX5 was higher in MdWOX11-OE transgenic plants than in GL3 and MdWOX11-RNAi transgenic plants. …”
Publicado 2022
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1978por Liu, Bingchuan, Li, Xingcai, Qiu, Weipeng, Liu, Zhongjun, Zhou, Fang, Zheng, Yufeng, Wen, Peng, Tian, Yun“…Nevertheless, when the prosthesis was fixed by screws alone, no dense new callus could be observed. This fixation mode was optional for defects extremely close to the articular surface. …”
Publicado 2022
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1979por Polikarpova, A., Ellinghaus, A., Schmidt-Bleek, O., Grosser, L., Bucher, C. H., Duda, G. N., Tanaka, E. M., Schmidt-Bleek, K.“…Plate-fixated femurs regained bone integrity more efficiently in comparison to the non-fixated osteotomized bone, where larger callus formed, possibly to compensate for the bone fragment misalignment. …”
Publicado 2022
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1980por Andreasson, Erik, Kieu, Nam Phuong, Zahid, Muhammad Awais, Carlsen, Frida Meijer, Marit, Lenman, Sandgrind, Sjur, Petersen, Bent Larsen, Zhu, Li-Hua“…Utilization of protoplasts has become a more important approach for obtaining transgene-free edited plants by genome editing, CRISPR/Cas9. In this approach, callus formation from protoplasts is induced by one set of hormones, followed by organogenesis, i.e., shoot formation, which is induced by a second set of hormones. …”
Publicado 2022
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