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Mostrando 221 - 240 Resultados de 334 Para Buscar '"Catcher"', tiempo de consulta: 0.65s Limitar resultados
  1. 221
    “…The inCell‐AEH nanoparticles were generated from polycistronic vectors enabling simultaneous expression of two interacting enzymes, the Lactobacillus brevis alcohol dehydrogenase (ADH) and the Bacillus subtilis glucose‐1‐dehydrogenase (GDH), fused with a SpyCatcher or SpyTag, respectively. Formation of inCell‐AEH was analyzed by dynamic light scattering and atomic force microscopy. …”
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    Online Artículo Texto
    Intracellular Assembly of Interacting Enzymes Yields Highly‐Active Nanoparticles for Flow Biocatalysis
  2. 222
    por Adloff, C., Blaha, J., Blaising, J.J., Drancourt, C., Espargiliere, A., Gaglione, R., Geffroy, N., Karyotakis, Y., Prast, J., Vouters, G., Francis, K., Repond, J., Smith, J., Xia, L., Baldolemar, E., Li, J., Park, S.T., Sosebee, M., White, A.P., Yu, J., Buanes, T., Eigen, G., Mikami, Y., Watson, N.K., Goto, T., Mavromanolakis, G., Thomson, M.A., Ward, D.R., Yan, W., Benchekroun, D., Hoummada, A., Khoulaki, Y., Benyamna, M., Carloganu, C., Fehr, F., Gay, P., Manen, S., Royer, L., Blazey, G.C., Dyshkant, A., Lima, J.G.R., Zutshi, V., Hostachy, J.Y., Morin, L., Cornett, U., David, D., Falley, G., Gadow, K., Gottlicher, P., Gunter, C., Hermberg, B., Karstensen, S., Krivan, F., Lucaci-Timoce, A.I., Lu, S., Lutz, B., Morozov, S., Morgunov, V., Reinecke, M., Sefkow, F., Smirnov, P., Terwort, M., Vargas-Trevino, A., Feege, N., Garutti, E., Marchesini, I., Ramilli, M., Eckert, P., Harion, T., Kaplan, A., Schultz-Coulon, H.Ch, Shen, W., Stamen, R., Tadday, A., Bilki, B., Norbeck, E., Onel, Y., Wilson, G.W., Kawagoe, K., Dauncey, P.D., Magnan, A.M., Wing, M., Salvatore, F., Calvo Alamillo, E., Fouz, M.C., Puerta-Pelayo, J., Balagura, V., Bobchenko, B., Chadeeva, M., Danilov, M., Epifantsev, A., Markin, O., Mizuk, R., Novikov, E., Rusinov, V., Tarkovsky, E., Kirikova, N., Kozlov, V., Smirnov, P., Soloviev, Y., Buzhan, P., Dolgoshein, B., Ilyin, A., Kantserov, V., Kaplin, V., Karakash, A., Popova, E., Smirnov, S., Kiesling, C., Pfau, S., Seidel, K., Simon, F., Soldner, C., Szalay, M., Tesar, M., Weuste, L., Bonis, J., Bouquet, B., Callier, S., Cornebise, P., Doublet, Ph, Dulucq, F., Faucci Giannelli, M., Fleury, J., Li, H., Martin-Chassard, G., Richard, F., de la Taille, Ch., Poschl, R., Raux, L., Seguin-Moreau, N., Wicek, F., Anduze, M., Boudry, V., Brient, J.C., Jeans, D., Mora de Freitas, P., Musat, G., Reinhard, M., Ruan, M., Videau, H., Bulanek, B., Zacek, J., Cvach, J., Gallus, P., Havranek, M., Janata, M., Kvasnicka, J., Lednicky, D., Marcisovsky, M., Polak, I., Popule, J., Tomasek, L., Tomasek, M., Ruzicka, P., Sicho, P., Smolik, J., Vrba, V., Zalesak, J., Belhorma, B., Ghazlane, H., Takeshita, T., Uozumi, S., Sauer, J., Weber, S., Zeitnitz, C.
    Publicado 2012
    “…The hadronic energy resolution of the CALICE setup, consisting of a silicon tungsten electromagnetic calorimeter, an analog hadron calorimeter and an analog tail catcher has been studied using data taken in 2007 at CERN and simulations. …”
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    Hadronic energy resolution of a highly granular scintillator-steel hadron calorimeter using software compensation techniques
  3. 223
    “…METHODS: In this study, two well-studied antigen candidates, PcrV and OprI, were selected and connected to the ferritin nanoparticle by the Spytag/SpyCatcher system to generate the nanovaccine rePO-FN. …”
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    Online Artículo Texto
    Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine
  4. 224
    “…We optimized and employed the recently developed SpyTag/SpyCatcher technique to establish a protein assembly system for vaccine generation from pre-prepared subunit proteins. …”
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    Online Artículo Texto
    A novel method for synthetic vaccine construction based on protein assembly
  5. 225
    “…We observe that the derived thickness is of similar magnitude under 1 bar and under vacuum conditions. A catcher unit facilitates the recycling of the solutions, allowing measurements on small sample volumes (∼10 ml). …”
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    Online Artículo Texto
    A liquid flatjet system for solution phase soft-x-ray spectroscopy
  6. 226
    “…Terbium-155 prepared by proton irradiation and on-line mass separation at the CERN-ISOLDE and CERN-MEDICIS facilities contains radioactive (139)Ce(16)O and also zinc or gold, depending on the catcher foil used. A method using ion-exchange and extraction chromatography resins in two column separation steps has been developed to isolate (155)Tb with a chemical yield of ≥95% and radionuclidic purity ≥99.9%. …”
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    Online Artículo Texto
    Chemical Purification of Terbium-155 from Pseudo-Isobaric Impurities in a Mass Separated Source Produced at CERN
  7. 227
    “…It has been suggested that B-cells with receptors that recognize conserved epitopes would be preferentially stimulated through avidity effects by mosaic particles presenting multiple forms of a variable antigen. We adapted SpyCatcher-based platforms, AP205 virus-like particles (VLPs) and mi3 nanoparticles (NPs), to covalently co-display SpyTagged hemagglutinin (HA) trimers from group 1 and group 2 influenza A strains. …”
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    Online Artículo Texto
    Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers
  8. 228
    “…Here, we show that ferritin‐like Dps from hyperthermophilic Sulfolobus islandicus, covalently coupled with SARS‐CoV‐2 antigens via the SpyCatcher system, forms stable multivalent dodecameric vaccine nanoparticles that remain intact even after lyophilisation. …”
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    Online Artículo Texto
    Single‐dose immunisation with a multimerised SARS‐CoV‐2 receptor binding domain (RBD) induces an enhanced and protective response in mice
  9. 229
    “…In the present work, we adapted a previously designed non-infectious adenovirus-inspired 60-mer dodecahedric VLP (ADDomer) to display a multimeric array of large antigens through a SpyTag/SpyCatcher system. To validate the platform as a potential COVID-19 vaccine approach, we decorated the newly designed VLP with the glycosylated receptor binding domain (RBD) of SARS-CoV-2. …”
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    Online Artículo Texto
    Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization with a versatile adenovirus-inspired multimerization platform
  10. 230
    “…In this study, we used the versatile Tag/Catcher AP205 capsid virus-like particle (cVLP) vaccine platform to develop an IL-1β-targeted vaccine and to assess the immunogenicity and in vivo efficacy of the vaccine in a translational mouse model of ACD. …”
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    Online Artículo Texto
    Preclinical Efficacy of a Capsid Virus-like Particle-Based Vaccine Targeting IL-1β for Treatment of Allergic Contact Dermatitis
  11. 231
    “…Herein, a feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher (ST/SC)-mediated anchoring is presented. Carboxyl functional groups on the surface of silica-coated magnetic beads (SMBs) were coupled with SC using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysulfosuccinimide method, named SC-SMBs. …”
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    Online Artículo Texto
    A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing
  12. 232
    “…These yeast cells have been genetically engineered to display the protein pairs SpyTag/SpyCatcher or CL7/Im7 on their surfaces, which enable their assembly into multicellular structures capable of further growth and proliferation. …”
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    Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
  13. 233
    por Hayes, Heather C., Luk, Louis Y. P.
    Publicado 2023
    “…Here, the plastic-degrading enzyme IsPETase was cyclized in a range of topologies including a cyclic monomer, cyclic dimer and catenane using SpyTag/SpyCatcher technologies, and their tolerance towards different stresses including mechanical agitation was investigated. …”
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    Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation
  14. 234
    “…To effectively utilize the high beam power of the driver two-step target /ion source geometries are proposed: (1) Neutron production with intermediate energy deuterons on $9 a primary target to produce neutron- rich fission products in a secondary /sup 238/U target, and (2) Fragmentation of neutron-rich heavy ion beams such as /sup 18/O in a target/catcher geometry. Heavy ion beams with total energies in $9 the 1-10 GeV range are also available for radionuclide production via high-energy spallation reactions: at the present time R&D is in progress to develop superconducting resonator structures for a driver linac to cover the energy $9 range up to 100 MeV per nucleon for heavy ions and 200 MeV for protons. …”
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    An advanced ISOL facility based on ATLAS
  15. 235
    “…Here, we developed a strategy to generate cells that display PhyB as a membrane-bound protein by expressing the SpyCatcher fused to a transmembrane domain in HEK-293T cells and covalently coupling purified PhyB-SpyTag to these cells. …”
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    Online Artículo Texto
    Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions
  16. 236
    “…The VapA and capsid proteins were expressed individually in E. coli and VapA was fused to auto-assembled VLPs using the SpyTag/SpyCatcher technology. Rainbow trout were vaccinated/immunized with the VapA-VLP vaccines by intraperitoneal injection and were challenged with A. salmonicida 7 weeks later. …”
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    High immunogenicity of virus-like particles (VLPs) decorated with Aeromonas salmonicida VapA antigen in rainbow trout
  17. 237
    por Sukupayo, Punya Ram, Tamang, Semsal
    Publicado 2023
    “…The samples from pet dogs were collected from the ground immediately after voiding with the help of the owner, whereas stray dogs were caught by using a dog catcher, and the samples were collected directly from the rectum by using a gloved index finger. …”
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    Prevalence of Zoonotic Gastrointestinal Helminth Parasite among Dogs in Suryabinayak, Nepal
  18. 238
    “…This study aimed to compare the applicability of 5 fusion calling pipelines (Arriba, deFuse, CICERO, FusionCatcher, and STAR-Fusion), as well as to define and develop an integrative bioinformatics pipeline (Fusion InPipe) to detect clinically relevant gene fusions in acute pediatric leukemia. …”
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    Fusion InPipe, an integrative pipeline for gene fusion detection from RNA-seq data in acute pediatric leukemia
  19. 239
    “…In addition, the prepared cross-linked chitosan bis-aldehyde derivatives were examined as U(VI) catcher from waste solutions. GRAPHICAL ABSTRACT: [Image: see text]…”
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    An effective uranium removal using diversified synthesized cross-linked chitosan bis-aldehyde Schiff base derivatives from aqueous solutions
  20. 240
    “…Towards a generic route to thermo-resilience, we established the SpyRing approach, cyclizing enzymes by sandwiching between SpyTag and SpyCatcher (peptide and protein partners which lock together via a spontaneous isopeptide bond). …”
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    SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries
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