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261por Chen, Manyun, Shu, Yan, Li, Qing, Kang, Zhiqiang, Liu, Tao, Zhou, Honghao, Huang, Weihua, Zhang, Wei“…Here we report the B. ovatus as an effective VB12 catcher which was enriched in the type 2 diabetes patients suffered from VB12 deficiency after 3 to 6 months of metformin treatment. …”
Publicado 2023
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262por Kawa, S., Tokoo, M., Hasebe, O., Hayashi, K., Imai, H., Oguchi, H., Kiyosawa, K., Furuta, S., Homma, T.“…This unfavourable result is attributed to the C241 catcher antibody of this assay system, which has almost the same epitope specificity as the C50 and the NS19-9 monoclonal antibodies. …”
Publicado 1994
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263por Liu, Silvia, Tsai, Wei-Hsiang, Ding, Ying, Chen, Rui, Fang, Zhou, Huo, Zhiguang, Kim, SungHwan, Ma, Tianzhou, Chang, Ting-Yu, Priedigkeit, Nolan Michael, Lee, Adrian V., Luo, Jianhua, Wang, Hsei-Wei, Chung, I-Fang, Tseng, George C.“…No single method dominantly performed the best but SOAPfuse generally performed well, followed by FusionCatcher and JAFFA. We further demonstrated the potential of a meta-caller algorithm by combining top performing methods to re-prioritize candidate fusion transcripts with high confidence that can be followed by experimental validation. …”
Publicado 2016
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264por Vargas Paris, Roberto, Skorpil, Mikael, Westerlund, Eli, Lindholm, Peter, Nyrén, Sven“…It could potentially be used as an eye catcher, i.e. where to look for PE in other MRI sequences.…”
Publicado 2018
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265por Cho, Jooyoung, Kim, Young Sam, Kim, Young Hwan, Lee, Jae-Yeon, Bae, In Cheol, Lee, Sang-Guk, Kim, Jeong-Ho“…METHODS: The i-STAT G3+ (Abbott) was used as a reference analyzer to evaluate the pH values obtained from other methods: the i-Smart 300 (i-SENS), the pHOx Ultra (Nova Biomedical), using a clot catcher to filter off microclot, and pH indicator paper. …”
Publicado 2019
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266por Lampinen, Vili, Heinimäki, Suvi, Laitinen, Olli H., Pesu, Marko, Hankaniemi, Minna M., Blazevic, Vesna, Hytönen, Vesa P.“…The conserved influenza antigens were produced separately as SpyCatcher fusions in E. coli before covalent conjugation on the surface of noro-VLP. …”
Publicado 2021
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267por Shitara, Hitoshi, Tajika, Tsuyoshi, Kuboi, Takuro, Ichinose, Tsuyoshi, Sasaki, Tsuyoshi, Hamano, Noritaka, Endo, Takafumi, Kamiyama, Masataka, Honda, Akira, Miyamoto, Ryosuke, Nakase, Kurumi, Yamamoto, Atsushi, Kobayashi, Tsutomu, Takagishi, Kenji, Chikuda, Hirotaka“…Univariate analysis showed that age, height, weight, ROM of elbow flexion in the dominant arm, muscle strength ratio of shoulder abduction, and the likelihood of being a pitcher or a catcher were significantly greater in the injured group than in the non-injured group. …”
Publicado 2021
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268por He, Linling, Lin, Xiaohe, Wang, Ying, Abraham, Ciril, Sou, Cindy, Ngo, Timothy, Zhang, Yi, Wilson, Ian A., Zhu, Jiang“…We first displayed the receptor-binding domain (RBD) on three self-assembling protein nanoparticle (SApNP) platforms using the SpyTag/SpyCatcher system. We then identified heptad repeat 2 (HR2) in S2 as the cause of spike metastability, designed an HR2-deleted glycine-capped spike (S2GΔHR2), and displayed S2GΔHR2 on SApNPs. …”
Publicado 2021
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269por Cheng, Keman, Zhao, Ruifang, Li, Yao, Qi, Yingqiu, Wang, Yazhou, Zhang, Yinlong, Qin, Hao, Qin, Yuting, Chen, Long, Li, Chen, Liang, Jie, Li, Yujing, Xu, Jiaqi, Han, Xuexiang, Anderson, Gregory J., Shi, Jian, Ren, Lei, Zhao, Xiao, Nie, Guangjun“…We first display tumor antigens on the OMVs surface by fusing with ClyA protein, and then simplify the antigen display process by employing a Plug-and-Display system comprising the tag/catcher protein pairs. OMVs decorated with different protein catchers can simultaneously display multiple, distinct tumor antigens to elicit a synergistic antitumour immune response. …”
Publicado 2021
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270por Li, Liyan, Miao, Qianwei, Meng, Fanqiang, Li, Baoqi, Xue, Tianyuan, Fang, Tianliang, Zhang, Zhirang, Zhang, Jinxie, Ye, Xinyu, Kang, Yang, Zhang, Xingding, Chen, Qian, Liang, Xin, Chen, Hongbo, Zhang, Xudong“…Herein, we prepared cluster of differentiation 64 (CD64), a natural catcher of the fragment crystalline (Fc) of monomeric immunoglobulin G (IgG), and over-expressed it on the cell membrane nanovesicles (NVs) as PD-L1 antibody delivery vehicle (CD64-NVs-aPD-L1), which was employed to disrupt the PD-1/PD-L1 immunosuppressive signal axis for boosting T cell dependent tumor elimination. …”
Publicado 2021
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271por van Oosten, Linda, Altenburg, Jort J., Fougeroux, Cyrielle, Geertsema, Corinne, van den End, Fred, Evers, Wendy A. C., Westphal, Adrie H., Lindhoud, Simon, van den Berg, Willy, Swarts, Daan C., Deurhof, Laurens, Suhrbier, Andreas, Le, Thuy T., Torres Morales, Shessy, Myeni, Sebenzile K., Kikkert, Marjolein, Sander, Adam F., de Jongh, Willem Adriaan, Dagil, Robert, Nielsen, Morten A., Salanti, Ali, Søgaard, Max, Keijzer, Timo M. P., Weijers, Dolf, Eppink, Michel H. M., Wijffels, René H., van Oers, Monique M., Martens, Dirk E., Pijlman, Gorben P.“…Since subunit S only partially protected mice from SARS-CoV-2 challenge, we produced S1 for conjugation to bacteriophage AP205 VLP nanoparticles using tag/catcher technology. The S1 yield in an insect-cell bioreactor was ∼11 mg/liter, and authentic protein folding, efficient glycosylation, partial trimerization, and ACE2 receptor binding was confirmed. …”
Publicado 2021
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272por Stenson, James F., Cheesman, Quincy T., Kirsch, Jacob M., Antonacci, Christopher L., Alberta, Frank G., Austin, Luke S.“…Seventeen were pitchers, and 1 was a catcher. Level of play included 12 collegiate athletes, 2 high school athletes, 2 recreational athletes, 1 minor league athletes, and 1 major league athlete. …”
Publicado 2021
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273por Orozco-Hidalgo, Maria Teresa, Charrier, Marimikel, Tjahjono, Nicholas, Tesoriero, Robert F., Li, Dong, Molinari, Sara, Ryan, Kathleen R., Ashby, Paul D., Rad, Behzad, Ajo-Franklin, Caroline M.“…We engineered Caulobacter crescentus to secrete an extracellular matrix protein composed of an elastin-like polypeptide (ELP) hydrogel fused to supercharged SpyCatcher [SC((−))]. This biopolymer was secreted at levels of 60 mg/liter, an unprecedented level of biomaterial secretion by a native type I secretion apparatus. …”
Publicado 2021
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274por Schlör, Anja, Hirschberg, Stefan, Amor, Ghada Ben, Meister, Toni Luise, Arora, Prerna, Pöhlmann, Stefan, Hoffmann, Markus, Pfaender, Stephanie, Eddin, Omar Kamal, Kamhieh-Milz, Julian, Hanack, Katja“…The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. …”
Publicado 2022
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275por Decock, Anneleen, Creytens, David, Lefever, Steve, Van der Meulen, Joni, Anckaert, Jasper, De Ganck, Ariane, Deleu, Jill, De Wilde, Bram, Fierro, Carolina, Kuersten, Scott, Luypaert, Manuel, Rottiers, Isabelle, Schroth, Gary P., Steyaert, Sandra, Vanderheyden, Katrien, Vanden Eynde, Eveline, Verniers, Kimberly, Verreth, Joke, Van Dorpe, Jo, Vandesompele, Jo“…Fusion transcript calling by FusionCatcher confirmed these aberrations and enabled the identification of both fusion gene partners and breakpoints. …”
Publicado 2022
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276por Ramirez, Aaron, Felgner, Jiin, Jain, Aarti, Jan, Sharon, Albin, Tyler J., Badten, Alexander J., Gregory, Anthony E., Nakajima, Rie, Jasinskas, Algimantas, Felgner, Philip L., Burkhardt, Amanda M., Davies, D. Huw, Wang, Szu-Wen“…We investigated three approaches for conjugation of the immunodominant outer membrane protein antigen (CBU1910) to the E2 nanoparticle to obtain a consistent antigen orientation: direct genetic fusion, high affinity tris-NTA-Ni conjugation to polyhistidine-tagged CBU1910, and the SpyTag/SpyCatcher (ST/SC) system. Overall, we found that the ST/SC approach yielded nanoparticles loaded with the highest number of antigens while maintaining stability, enabling formulations that could simultaneously co-deliver the protein antigen (CBU1910) and adjuvant (CpG1826) on one nanoparticle (CBU1910-CpG-E2). …”
Publicado 2023
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277por Szöllősi, Dávid, Hajdrik, Polett, Tordai, Hedvig, Horváth, Ildikó, Veres, Dániel S., Gillich, Bernadett, Shailaja, Kanni Das, Smeller, László, Bergmann, Ralf, Bachmann, Michael, Mihály, Judith, Gaál, Anikó, Jezsó, Bálint, Barátki, Balázs, Kövesdi, Dorottya, Bősze, Szilvia, Szabó, Ildikó, Felföldi, Tamás, Oszwald, Erzsébet, Padmanabhan, Parasuraman, Gulyás, Balázs Zoltán, Hamdani, Nazha, Máthé, Domokos, Varga, Zoltán, Szigeti, Krisztián“…A novel bacterial strain (E. coli BL21(DE3) ΔnlpI, ΔlpxM) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. …”
Publicado 2023
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278por Song, Jinxing, Wang, Mengxiang, Zhou, Lei, Tian, Panpan, Sun, ZhuoYa, Sun, Junru, Wang, Xuannian, Zhuang, Guoqing, Jiang, Dawei, Wu, Yanan, Zhang, Gaiping“…In this study, we developed a self-assembled nano-ASFV vaccine candidate (NanoFVax) targeting DCs, by covalently coupling the self-assembled 24-mer ferritin with the dominant B and T cell epitopes of the highly immunogenic ASFV antigen (p72, CD2v, pB602L and p30) and fused with the chemokine receptor XCL1 (a DC targeting molecule) through the SpyTag/SpyCatcher protein ligase system. Compared to monomeric protein, the nanoparticle vaccines can induce a more robust T-cell response, and the high-level antibody response against ASFV can last for more than 231 days. …”
Publicado 2023
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279por Dorsch, Oliver, Krieter, Detlef H, Lemke, Horst-Dieter, Fischer, Stefan, Melzer, Nima, Sieder, Christian, Bramlage, Peter, Harenberg, Job“…The percentage of unsatisfactory dialysis results at 8 weeks due to clotting or bleeding, was 1.9% (n = 2/106; 95% confidence interval [CI] 0.23–6.65%); no major bleeding. 1.9% had moderate/severe clotting in the lines/bubble catcher and 2.8% in the dialyser at week 8. 15.7 ± 14.3% of the dialysis filters’ visual surface area was showing redness. …”
Publicado 2012
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280por Yenkoidiok-Douti, Lampouguin, Williams, Adeline E., Canepa, Gaspar E., Molina-Cruz, Alvaro, Barillas-Mury, Carolina“…We report the development and evaluation of a Pfs47-based virus-like particle (VLP) vaccine generated by conjugating our 58 amino acid Pfs47 antigen to Acinetobacter phage AP205-VLP using the SpyCatcher:SpyTag adaptor system. AP205-Pfs47 complexes (VLP-P47) formed particles of ~22 nm diameter that reacted with polyclonal anti-Pfs47 antibodies, indicating that the antigen was accessible on the surface of the particle. …”
Publicado 2019
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