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281por Su, Jiulong, Kitaguchi, Tetsuya, Ohmuro-Matsuyama, Yuki, Seah, Theresa, Ghadessy, Farid J., Hoon, Shawn, Ueda, Hiroshi“…Additionally, through use of an anti-caffeine V(HH)-SpyCatcher fusion protein, caffeine could be detected using SpyTag-fused TM-IV5(m) protein expressed in protocells, suggesting utility of this platform for detection of diverse antigen types.…”
Publicado 2019
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282por Beer, Martin, Amery, Leanne, Bosch, Berend-Jan, Brix, Alexander, Daramola, Olalekan, Inman, Sophie, Jungbäck, Carmen, Kortekaas, Jeroen, Lindo, Viv, Okorji-Obike, Uche, Rodriguez-Conde, Sara, Tang, Alison, Tchelet, Ronen, Vandeputte, Joris, Wichgers Schreur, Paul J., Osterhaus, Ab, Haagmans, Bart, Audonnet, Jean-Christophe“…For vaccine production, viral antigens were fused to multimeric protein scaffold particles using the SpyCatcher/SpyTag system. In vivo models demonstrated the efficacy of both antibodies and vaccines. …”
Publicado 2021
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283por Patel, DR, Minns, AM, Sim, DG, Field, CJ, Kerr, AE, Heinly, T, Luley, EH, Rossi, R.M., Bator, C., Moustafa, I.M., Hafenstein, SL, Lindner, SE, Sutton, TC“…The vaccine candidate consisted of the self-assembling 60-subunit I3-01 protein scaffold covalently decorated with the SARS-CoV-2 receptor binding domain (RBD) using the SpyCatcher-SpyTag system. We verified the intended antigen display features by reconstructing the I3-01 scaffold to 3.4A using cryo-EM, and then demonstrated that the scaffold was highly saturated when grafted with RBD. …”
Publicado 2022
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284por Biasiolo, Alessandra, Tono, Natascia, Ruvoletto, Mariagrazia, Quarta, Santina, Turato, Cristian, Villano, Gianmarco, Beneduce, Luca, Fassina, Giorgio, Merkel, Carlo, Gatta, Angelo, Pontisso, Patrizia“…Monoclonal antibodies against serpinB3 and serpinB4 were used as catcher antibodies to set up specific ELISA assays, while total SCCA-IgM immunocomplexes were detected by commercially available ELISA assay. …”
Publicado 2012
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285por Rodrigues, Marianni de Moura, Marques, Gisela Rita Alvarenga Monteiro, Serpa, Lígia Leandro Nunes, Arduino, Marylene de Brito, Voltolini, Júlio Cesar, Barbosa, Gerson Laurindo, Andrade, Valmir Roberto, de Lima, Virgília Luna Castor“…METHODS: Adult mosquitoes were collected monthly with a hand net and portable electric catcher in the peridomiciliary and intradomiciliary premises of residences in an urban area with ongoing dengue transmission in the municipality of São Sebastião, Brazil, from February 2011 to February 2012. …”
Publicado 2015
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286“…After an average follow-up of 30 months (range 24-42 months), none of the patients had recurrence of the painful snapping of their tendon, and all 14 competitive athletes returned to full participation in their sport which included Division 1-A crew and soccer (she played 90 mins/game), and high school basketball and softball (she played catcher), at an average of 8.8 months (range 4-12 months) after surgery. …”
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287“…METHODS: Thirty-three right-handed youth male baseball pitchers (mean ± SD: age, 13.6 ± 2.0 years; height, 169.4 ± 14.3 cm; weight, 63.5 ± 13.0 kg; experience, 7.3 ± 3.0 years) threw 3 fastballs to a catcher while kinematic data were collected with an electromagnetic tracking system. …”
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288“…Pitching >100 innings in 1 year, being aged 9 to 11 years, being a pitcher or catcher, training >16 hours per week, and having a history of elbow pain were significant risk factors for elbow injury among youth players. …”
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289por Fougeroux, Cyrielle, Goksøyr, Louise, Idorn, Manja, Soroka, Vladislav, Myeni, Sebenzile K., Dagil, Robert, Janitzek, Christoph M., Søgaard, Max, Aves, Kara-Lee, Horsted, Emma W., Erdoğan, Sayit Mahmut, Gustavsson, Tobias, Dorosz, Jerzy, Clemmensen, Stine, Fredsgaard, Laurits, Thrane, Susan, Vidal-Calvo, Elena E., Khalifé, Paul, Hulen, Thomas M., Choudhary, Swati, Theisen, Michael, Singh, Susheel K., Garcia-Senosiain, Asier, Van Oosten, Linda, Pijlman, Gorben, Hierzberger, Bettina, Domeyer, Tanja, Nalewajek, Blanka W., Strøbæk, Anette, Skrzypczak, Magdalena, Andersson, Laura F., Buus, Søren, Buus, Anette Stryhn, Christensen, Jan Pravsgaard, Dalebout, Tim J., Iversen, Kasper, Harritshøj, Lene H., Mordmüller, Benjamin, Ullum, Henrik, Reinert, Line S., de Jongh, Willem Adriaan, Kikkert, Marjolein, Paludan, Søren R., Theander, Thor G., Nielsen, Morten A., Salanti, Ali, Sander, Adam F.“…RBD antigens are displayed on AP205 CLPs through a split-protein Tag/Catcher, ensuring unidirectional and high-density display of RBD. …”
Publicado 2021
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290“…All participants threw 3 fastballs to a catcher for a strike at regulation distance. Owing to nonnormally distributed data, Mann-Whitney U tests were used to determine group differences at 5 events during the pitching motion. …”
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291por Kerbs, Paul, Vosberg, Sebastian, Krebs, Stefan, Graf, Alexander, Blum, Helmut, Swoboda, Anja, Batcha, Aarif M. N., Mansmann, Ulrich, Metzler, Dirk, Heckman, Caroline A., Herold, Tobias, Greif, Philipp A.“…To evaluate the performance of fusion gene detection by RNAsequencing compared to standard diagnostic techniques, we analyzed 806 RNA-sequencing samples from patients with acute myeloid leukemia using two state-of-the-art software tools, namely Arriba and FusionCatcher. RNA-sequencing detected 90% of fusion events that were reported by routine with high evidence, while samples in which RNA-sequencing failed to detect fusion genes had overall lower and inhomogeneous sequence coverage. …”
Publicado 2021
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292por Uffmann, William J., ElAttrache, Neal S., Youssefzadeh, Keon, Carbone, Andrew, Limpisvasti, Orr“…There were 9 pitchers, 1 catcher, and 1 outfielder. All patients had debridement of the posterosuperior labrum and rotator cuff. …”
Publicado 2023
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293“…Of the 58 transportation injuries most occurred in catchers (93%) and smaller vessels (1 to 3 crew) (55%). …”
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294“…Playing positions included pitcher (21 patients), catcher (3 patients), infielder (13 patients) and outfielder (8 patients). …”
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295por Karan, Ram, Renn, Dominik, Nozue, Shuho, Zhao, Lingyun, Habuchi, Satoshi, Allers, Thorsten, Rueping, Magnus“…We used a thiol–maleimide chemistry strategy to introduce the biotechnological relevant activity by maleimide-activated streptavidin–biotin and maleimide-activated SpyTag003-SpyCatcher003 mediated functionalization of GVNPs. CONCLUSION: The merger of these genetic and chemical functionalization approaches significantly extends these novel protein nanomaterials' bioengineering and functionalization potential to assemble catalytically active proteins, biomaterials, and vaccines onto one nanoparticle in a modular fashion. …”
Publicado 2023
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296por Norouzi, Masoud, Truong, Thang, Jaenes, Katariina, Warner, Bryce M., Vendramelli, Robert, Tierney, Kevin, Kobasa, Darwyn, Tailor, Nikesh, Plant, Pamela, dos Santos, Claudia, Babiuk, Shawn, Ambagala, Aruna, Pardee, Keith“…Similar in mechanism to the widely used enzyme-linked immunosorbent assays (ELISAs), our method is novel and advantageous in that (i) it uses linear DNA to produce the target viral antigen fused to a SpyTag peptide in a cell-free expression system without the need for traditional cloning and antigen purification, (ii) it uses SpyCatcher2-Apex2, an Escherichia coli-produced peroxidase conjugate as a universal secondary detection reagent, obviating the need for commercial or sophisticated enzyme conjugates, and (iii) sera are spotted directly on a nitrocellulose membrane, enabling a simple “dipping” mechanism for downstream incubation and washing steps, as opposed to individual processing of wells in a multiwell plate. …”
Publicado 2023
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297por Oliver, Gretchen D., Downs, Jessica L., Friesen, Kenzie B., Anz, Adam W., Dugas, Jeff R., Andrews, James R.“…After warm-up, participants were instructed to throw random game situations, provided by the investigator, with the catcher located at regulation distance (43ft). Participants were required to produce three outs an inning as per a regulation softball game. …”
Publicado 2020
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298por Tanaka, Hiroshi, Hayashi, Toyohiko, Inui, Hiroaki, Ninomiya, Hiroki, Muto, Tomoyuki, Nobuhara, Katsuya“…After performing a preparation routine of stretching and warm-up pitching, each player pitch to 5 fastball pitches off the pitching mound to a catcher at the regulation distance of 16 m for youth pitchers. …”
Publicado 2016
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299“…We employed the ultrastable filamentous protein gamma-prefoldin as a material scaffold that can be functionalized with a variety of protein domains through SpyTag/SpyCatcher conjugation chemistry. The purification of recombinant gamma-prefoldin filaments from bacterial lysates using MMC was tested across a wide range of salt concentrations and pH, demonstrating that the MMC resin is robust, however the optimal choice of salt species, salt concentration, and pH is likely dependent on the protein nanostructure to be purified. …”
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300“…The smart collimator and cryo-catcher were designed, built and successfully tested. …”
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info:eu-repo/semantics/article