“…Catching and thinning are acknowledged as threats to the maintenance of good biosecurity, yet the people employed to undertake this critical work (i.e. ‘catchers’) are a rarely studied group. This study uses a mixed methods approach to investigate catchers’ (n = 53) understanding of the biosecurity threats posed by the catching and thinning, and the barriers to good biosecurity practice. …”
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“…A promising approach relies on so-called tag/catcher systems that are fused to the proteins of interest and allow a durable linkage via covalent intermolecular bonds. …”
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“…SpyCatcher002 was fused to an intimin derived from enterohemorrhagic Escherichia coli. …”
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“…Herein, we describe a modular approach, in which the membrane anchor protein Lpp‐OmpA and a protein of interest (passenger) are expressed independently as genetically fused SpyCatcher and SpyTag units and assembled in situ by post‐translational coupling. …”
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“…G-CatchER(+) exhibits a superior Ca(2+) on rate to G-CEPIA1er and has a Ca(2+)-induced fluorescence lifetimes increase. …”
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“…Together, the remarkable performance of SpyCatcher-SpyTag in nanodisc circularization paves the way for the use of cNDs in membrane biochemistry and structural biology.…”
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“…A prototype module for an International Linear Collider (ILC) detector was built, installed, and tested between 2006 and 2009 at CERN and Fermilab as part of the CALICE test beam program, in order to study the possibilities of extending energy sampling behind a hadronic calorimeter and to study the possibilities of providing muon tracking. The "tail catcher/muon tracker" (TCMT) is composed of 320 extruded scintillator strips (dimensions 1000 mm x 50 mm x 5 mm) packaged in 16 one-meter square planes interleaved between steel plates. …”
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“…The hadronic energy resolution of the CALICE setup, consisting of a silicon tungsten electromagnetic calorimeter, an analog hadron calorimeter and an analog tail catcher has been studied using data taken in 2007 at CERN and simulations. …”
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“…The data presented in this article were the basis for the study reported in the research articles entitled ‘Climate responsive behaviour heat pipe technology for enhanced passive airside cooling’ by Chaudhry and Hughes [10] which presents the passive airside cooling capability of heat pipes in response to gradually varying external temperatures and related to the research article “CFD and wind tunnel study of the performance of a uni-directional wind catcher with heat transfer devices” by Calautit and Hughes [1] which compares the ventilation performance of a standard roof mounted wind catcher and wind catcher incorporating the heat pipe technology. …”
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“…Genetically encoded covalent peptide tagging technology, such as the SpyTag–SpyCatcher reaction, has emerged as a unique way to do chemistry with proteins. …”
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“…Currently, no scientific consensus exists on the relative safety of catcher mask styles and materials. Due to differences in mass and material properties, the style and material of a catcher mask influences the impact metrics observed during simulated foul ball impacts. …”
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“…In this paper, we propose JamCatcher, a mobile jammer localization scheme for defending the AMI. …”
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“…Finally, we discuss how split-protein Tag/Catcher conjugation systems can help to further propagate and enhance modular CLP vaccine designs.…”
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“…Our method is publicly available as an open‐source repository at https://github.com/yoterel/iCatcher.…”
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“…To leverage the inherent strengths of both the mRNA and adenovirus platforms, we developed a novel modular adenoviral mRNA delivery platform based on Tag/Catcher bioconjugation. Specifically, we engineered adenoviral vectors integrating Tag/Catcher proteins at specific locales on the Ad capsid proteins, allowing us to anchor mRNA to the surface of engineered Ad viruses. …”
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“…The SpyTag serves as the covalent anchoring site for a target-cell-specific cell-binding protein (CBP) that is fused to a truncated SpyCatcher (SpyCatcherΔ). Target-cell-specific lentiviruses are created by mixing the Sind-SpyTag-pp and CBP-SpyCatcherΔ in vitro. …”
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“…We previously designed SpyTag, forming an irreversible bond with its protein partner SpyCatcher. SpyTag provides an easy route to anchor, bridge or multimerize proteins. …”
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“…The Dream Catcher test defines the criteria for a genuine discovery of the neural constituents of phenomenal consciousness. …”
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“…Here, we develop CopyCatcher systems in Drosophila to detect and quantify somatic gene conversion (SGC) events. …”
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“…In this study, we have developed a modified YSD method that utilises SpyTag/SpyCatcher-based in vivo protein ligation. A nanobody fused with a SpyTag of 16 amino acids and an anchor protein fused with a SpyCatcher of 113 amino acids are encoded by separate gene cassettes and then assembled via isopeptide bond formation. …”
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