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42“…An upgrade to the radioactive beam capability of the ATLAS facility has been proposed using 252Cf fission fragments thermalized and collected into a low-energy particle beam using a helium gas catcher. In order to reaccelerate these beams an existing ATLAS ECR ion source will be reconfigured as a charge breeder source. …”
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43por Wang, Jeffrey, de Vale, Joao Souza, Gupta, Saransh, Upadhyaya, Pulakesh, Lisboa, Felipe A., Schobel, Seth A., Elster, Eric A., Dente, Christopher J., Buchman, Timothy G., Kamaleswaran, Rishikesan“…This data was then used to fine-tune ClotCatcher, a novel deep learning model. ClotCatcher was validated on both the EUH dataset alone and GMH dataset alone. …”
Publicado 2023
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45“…The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. …”
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46por Fierle, Julie K., Abram-Saliba, Johan, Brioschi, Matteo, deTiani, Mariastella, Coukos, George, Dunn, Steven M.“…In this report, we describe a simple, yet powerful strategy that exploits the properties of the SpyCatcher/SpyTag (SpyC/SpyT) covalent interaction to improve substantially the speed and efficiency in obtaining functional antibody clones of interest. …”
Publicado 2019
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47“…On the other hand, Ni-catchers can capture leaked nickel ions from NiTi alloy actively by chelation reaction. …”
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48“…In this study, we developed an open-source R package TrendCatcher (https://github.com/jaleesr/TrendCatcher), which applies the smoothing spline ANOVA model and break point searching strategy, to identify and visualize distinct dynamic transcriptional gene signatures and biological processes from longitudinal data sets. …”
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49por Kellmann, Sarah-Jane, Hentrich, Christian, Putyrski, Mateusz, Hanuschka, Hanh, Cavada, Manuel, Knappik, Achim, Ylera, Francisco“…Here we describe SpyDisplay, a phage display method in which the display is achieved via SpyTag/SpyCatcher protein ligation instead of genetically fusing the displayed protein to a phage coat protein. …”
Publicado 2023
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50“…[Image: see text] We previously designed a calcium sensor CatchER (a GFP-based Calcium sensor for detecting high concentrations in the high calcium concentration environment such as ER) with a capability for monitoring calcium ion responses in various types of cells. …”
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51por Wang, Jindan, Wang, Yilin, Wang, Xinzhe, Zhang, Dandan, Wu, Shuyu, Zhang, Guangya“…BACKGROUND: SpyTag is a peptide that can form an irreversible covalent linkage to its 12 kDa partner SpyCatcher via a spontaneous isopeptide bond. Herein, we fused SpyTag at the N-terminal of lichenase and SpyCatcher at C-terminal so that the termini of lichenase were locked together by the covalent interaction between the partners. …”
Publicado 2016
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52“…Recently, it has been proven that the split protein CnaB2 (SpyTag/SpyCatcher, ST/SC) from Streptococcus pyogenes can induce covalent conjugation rapidly and efficiently under various conditions. …”
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53por Akiba, Hiroki, Takayanagi, Kensuke, Kusano-Arai, Osamu, Iwanari, Hiroko, Hamakubo, Takao, Tsumoto, Kouhei“…Here, we describe a technology for generating biparatopic antibodies through two-step targeting using a pair of polypeptides, SpyTag and SpyCatcher, that spontaneously react to form a covalent bond between antibody fragments. …”
Publicado 2020
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54por Kushnarova-Vakal, Anastasiia, Äärelä, Antti, Huovinen, Tuomas, Virta, Pasi, Lamminmäki, Urpo“…Herein, we demonstrate the production of single-chain variable fragment antibodies with fused SpyCatcher (scFv-SpyCatcher, monovalent) and alkaline phosphatase-SpyCatcher (scFv-AP-SpyCatcher, bivalent) on C-terminus and their conjugation to SpyTag002-oligonucleotide in phosphate-buffered saline (PBS). …”
Publicado 2020
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55“…Here, we present MosaiCatcher v2, a standardised workflow and reference framework for single-cell SV detection using Strand-seq. …”
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56“…Here, we present MosaiCatcher v2, a standardized workflow and reference framework for single-cell SV detection using Strand-seq. …”
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57“…Here, we report 2 cases of catcher’s mask cranioplasty and their long-term outcomes. …”
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58por Kadkhodazadeh, Maryam, Mohajel, Nasir, Behdani, Mahdi, Baesi, Kazem, Khodaei, Behzad, Azadmanesh, Kayhan, Arashkia, Arash“…This study aimed to present a simple and versatile method for generating Ad-based bionanoparticles specific to target cells, using the SpyTag-SpyCatcher system. The SpyTag peptide was inserted into the HI loop of fiber-knob protein, which could act as a covalent anchoring site for a targeting moiety fused to a truncated SpyCatcher (SpyCatcherΔ) pair. …”
Publicado 2022
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59por Erel, Yotam, Shannon, Katherine Adams, Chu, Junyi, Scott, Kim, Struhl, Melissa Kline, Cao, Peng, Tan, Xincheng, Hart, Peter, Raz, Gal, Piccolo, Sabrina, Mei, Catherine, Potter, Christine, Jaffe-Dax, Sagi, Lew-Williams, Casey, Tenenbaum, Joshua, Fairchild, Katherine, Bermano, Amit, Liu, Shari“…In this article, we built on a system for automatic gaze annotation in young children, iCatcher, by engineering improvements and then training and testing the system (referred to hereafter as iCatcher+) on three data sets with substantial video and participant variability (214 videos collected in U.S. lab and field sites, 143 videos collected in Senegal field sites, and 265 videos collected via webcams in homes; participant age range = 4 months–3.5 years). …”
Publicado 2023
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60por Peyret, Hadrien, Ponndorf, Daniel, Meshcheriakova, Yulia, Richardson, Jake, Lomonossoff, George P.“…Conjugation in the cytosol was more efficient when SpyCatcher was displayed on tHBcAg VLPs instead of being fused to GFP. …”
Publicado 2020
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