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61por Yanase, Takumi, Okuda-Shimazaki, Junko, Asano, Ryutaro, Ikebukuro, Kazunori, Sode, Koji, Tsugawa, Wakako“…The aim of this research is the development of a versatile method to create a DET-type oxidoreductase complex based on the SpyCatcher/SpyTag technique by preparing SpyCatcher-fused heme c and SpyTag-fused non-DET-type oxidoreductases, and by the in vitro formation of DET-type oxidoreductase complexes. …”
Publicado 2023
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62por Naderer, Brigitte, Binder, Alice, Matthes, Jörg, Spielvogel, Ines, Forrai, Michaela“…CONCLUSIONS: Compared to non‐edible objects, food presentations seem to be eye‐catchers that immediately grab children's attention and they are also able to maintain this attention. …”
Publicado 2020
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63“…FINDINGS: Here, MorphoCatcher is introduced as a fast and simple web plugin for PrimerExplorer with a clear interface. …”
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64“…As anticipated, covalent conjugation of the SpyTag and SpyCatcher segments improved Split-GFP dimerization to further increase Cre recombinase activity in living cells. …”
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65“…In this study, we developed an open-source R package TrendCatcher (https://github.com/jaleesr/TrendCatcher), which applies the smoothing spline ANOVA model and break point searching strategy to identify and visualize distinct dynamic transcriptional gene signatures and biological processes from longitudinal datasets. …”
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66por Wang, Jinmei, Lu, Yuan, Cheng, Pengpeng, Zhang, Chuyue, Tang, Lan, Du, Lihua, Li, Jinghua, Ou, Zhimin“…Stereoselective CpCR and GDH were successfully fused with SpyCatcher and SpyTag, respectively, to obtain four enzyme modules, namely: SpyCatcher-CpCR, SpyCatcher-GDH, SpyTag-CpCR, and SpyTag-GDH, which were covalently coupled in vitro to form two types of hydrogel-like BESCs: CpCR-SpyCatcher-SpyTag-GDH and GDH-SpyCatcher-SpyTag-CpCR. …”
Publicado 2023
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67por Lampinen, Vili, Gröhn, Stina, Soppela, Saana, Blazevic, Vesna, Hytönen, Vesa P., Hankaniemi, Minna M.“…VLPs decorated with SpyCatcher-M2e and VLPs with direct M2 efusion were used to immunize mice. …”
Publicado 2023
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68por Alam, Md. Kausar, Brabant, Michelle, Viswas, Raja Solomon, Barreto, Kris, Fonge, Humphrey, Ronald Geyer, C.“…CONCLUSION: We used the SpyTag/SpyCatcher protein ligase system to ligate anti-HER3 scFv fused to a SpyCatcher at its C-termini to a Tri-SpyTag to construct Tr-scFv. …”
Publicado 2018
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69por Karimi Baba ahmadi, Mohammad, Mohammadi, Seyed Abolghasem, Makvandi, Manoochehr, Mamoueie, Morteza, Rahmati, Mohammad, Wood, David“…. • Column free purification of SpyCatcher protein enables develop a universal solid support for SpyTag protein purification. • This method is highly simple and applicable to other proteins.…”
Publicado 2020
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70por Tan, Tiong Kit, Rijal, Pramila, Rahikainen, Rolle, Keeble, Anthony H., Schimanski, Lisa, Hussain, Saira, Harvey, Ruth, Hayes, Jack W. P., Edwards, Jane C., McLean, Rebecca K., Martini, Veronica, Pedrera, Miriam, Thakur, Nazia, Conceicao, Carina, Dietrich, Isabelle, Shelton, Holly, Ludi, Anna, Wilsden, Ginette, Browning, Clare, Zagrajek, Adrian K., Bialy, Dagmara, Bhat, Sushant, Stevenson-Leggett, Phoebe, Hollinghurst, Philippa, Tully, Matthew, Moffat, Katy, Chiu, Chris, Waters, Ryan, Gray, Ashley, Azhar, Mehreen, Mioulet, Valerie, Newman, Joseph, Asfor, Amin S., Burman, Alison, Crossley, Sylvia, Hammond, John A., Tchilian, Elma, Charleston, Bryan, Bailey, Dalan, Tuthill, Tobias J., Graham, Simon P., Duyvesteyn, Helen M. E., Malinauskas, Tomas, Huo, Jiandong, Tree, Julia A., Buttigieg, Karen R., Owens, Raymond J., Carroll, Miles W., Daniels, Rodney S., McCauley, John W., Stuart, David I., Huang, Kuan-Ying A., Howarth, Mark, Townsend, Alain R.“…The vaccine is based on the display of coronavirus spike glycoprotein receptor-binding domain (RBD) on a synthetic virus-like particle (VLP) platform, SpyCatcher003-mi3, using SpyTag/SpyCatcher technology. …”
Publicado 2021
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71por Mitchell, Megan I., Ben‐Dov, Iddo Z., Liu, Christina, Ye, Kenny, Chow, Kar, Kramer, Yael, Gangadharan, Anju, Park, Steven, Fitzgerald, Sean, Ramnauth, Andrew, Perlin, David S., Donato, Michele, Bhoy, Emily, Manouchehri Doulabi, Ehsan, Poulos, Michael, Kamali‐Moghaddam, Masood, Loudig, Olivier“…Altogether our data highlight the sensitivity and versatility of EV‐CATCHER.…”
Publicado 2021
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72por Solomon, Viswas Raja, Barreto, Kris, Bernhard, Wendy, Alizadeh, Elahe, Causey, Patrick, Perron, Randy, Gendron, Denise, Alam, Md. Kausar, Carr, Adriana, Geyer, C. Ronald, Fonge, Humphrey“…The ΔN-SpyCatcher-DFO was reacted with nimotuzumab-SpyTag to obtain stable nimotuzumab-SpyTag-∆N-SpyCatcher-DFO. …”
Publicado 2020
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73por Katsura, Miki, Fukushima, Masaki, Kameyama, Ken-ichiro, Kokuho, Takehiko, Nakahira, Yoichi, Takeuchi, Kaoru“…In this study, we generated red-spotted grouper nervous necrosis virus (RGNNV) VLPs presenting the BVDV E2 protein (domain I to IIIb) of the Nose (BVDV-1) or KZ-91-CP (BVDV-2) strain by exploiting SpyTag/SpyCatcher technology. Mice immunized twice with 30 μg of RGNNV VLPs conjugated with 10 μg of E2 proteins of the Nose or KZ-91-CP strain with a 14-day interval elicited high (1:512,000 to 1:1,024,000) and moderate (1:25,600 to 1:102,400) IgG titers against E2 proteins of homologous and heterologous strains, respectively. …”
Publicado 2023
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77por Baumbach, JonathanTabla de Contenidos: “…The saint as a young man: The catcher in the rye…”
Publicado 1965
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78por Wong, Jin Xiang, Gonzalez-Miro, Majela, Sutherland-Smith, Andrew J., Rehm, Bernd H. A.“…To expand the design space of this general modular approach toward programmable multifunctionalization, e.g., one-pot construction of immobilized multienzyme cascade systems on PHA spheres, we designed various recombinant bimodular PHA spheres utilizing alternative Tag/Catcher pairs (e.g., SnoopTag/SnoopCatcher and SdyTag/SdyCatcher systems). …”
Publicado 2020
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79“…SpyCatcher-mi3 expressed solubly to high yields in Escherichia coli, giving more than 10-fold greater yield than a comparable phage-derived icosahedral nanoparticle, SpyCatcher-AP205. …”
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80“…In this paper, we applied the SpyTag/SpyCatcher technology as an alternative for the functionalization of TuMV VLPs with peptides and proteins. …”
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