“…First, the dual-chamber gas catcher is investigated. This gas catcher is separated in two volumes, one for the stopping and neutralising of the nuclear recoils and one for the laser ionisation. …”
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“…SpyTag is a peptide engineered to form a spontaneous isopeptide bond to its protein partner SpyCatcher. SpyTag or SpyCatcher was successfully fused to the C-terminus of Dead streptavidin subunits. …”
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“…In order to assure reliable operation with intermediate charge states, a special synchrotron design, including a catcher system for ionized beam ions had to be developed. …”
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“…We expressed in E. coli VLPs from the bacteriophage AP205 genetically fused to SpyCatcher. We demonstrated quantitative covalent coupling to SpyCatcher-VLPs after mixing with SpyTag-linked to malaria antigens, including CIDR and Pfs25. …”
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“…The SpyTag/SpyCatcher system forms a covalent bond, linking the target protein and a co-expressed MNase-SpyCatcher fusion construct. …”
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“…SpyChIP takes advantage of a specific covalent isopeptide bond that rapidly forms between the 15-amino acid SpyTag and the 17-kDa protein SpyCatcher. In SpyChIP, the target TF is fused with SpyTag by genome engineering, and an epitope tagged SpyCatcher is expressed in cell populations of interest, where it covalently binds to SpyTag-TF. …”
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“…Using radiochemical techniques we will ; a)~~measure the target fragment mass and charge distributions from the interaction of 86~MeV/A |1|2C with Ta, Bi and U; ; b)~~measure the target fragment forward momentum and average kinetic energy using the thick target-thick catcher technique for the above reactions; and ; c)~~measure the target fragment angular and differential energy distributions using thin target-thin catcher techniques for the reactions with Ta and U. \\ \\ These measurements should allow us to better characterize the transition between low energy and realistic heavy ion reaction mechanisms.…”
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“…Here we use a post-translational modification approach, using copper free click chemistry, to attach a SpyTag to a target protein molecule for attachment to a specific SpyCatcher modified BMC shell protein. We demonstrate that a post-translationally SpyTagged material can react with a SpyCatcher modified BMC and show its presence on the surface of BMCs, enabling future investigation of these structures as polyvalent antigen display scaffolds for vaccine development. …”
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“…We established an approach for coupling VLPs to diverse antigen symmetries. SpyCatcher003 enabled efficient VLP conjugation and extreme thermal resilience. …”
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“…For one-step organelle purification, recombinantly expressed SpyCatcher protein was immobilized on magnetic microbeads via covalent thiol-etherification. …”
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“…However, the effects of SpyTag/ SpyCatcher interaction with this enzyme are poorly understood, and their regulation of enzyme properties remains unclear. …”
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“…We previously engineered the diffusion-limited reactivity of the SpyTag003 peptide with its protein partner SpyCatcher003 through spontaneous isopeptide bond formation. …”
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“…We decorated OMVs with surface exposed SpyCatcher via a truncated OmpA(a.a 1-155)-SpyCatcher fusion. …”
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“…Here we describe a set of three computational tools, Cell Catcher, Mito Catcher and MiA, that we have developed to extract extensive mitochondrial network data on a single-cell level from multi-cell fluorescence images. …”
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“…Here we show that SpyCatcher can be immobilized covalently on GNPs through GST without the loss of its full functionality. …”
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“…Inserted SpyTags were shown to be accessible at the Salmonella OMV surface and to efficiently couple SpyCatcher-equipped fusion proteins. Next, we combined distally placed SnoopCatcher or SnoopTag sequences with internal SpyTags in a single Hbp molecule. …”
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“…These results reveal the potential of RBD‐SpyCatcher‐mi3 as a broadly protective vaccination strategy.…”
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“…Overall, our results demonstrate that SpyCatcher magnetosomes significantly expand the genetic toolbox for particle surface functionalization and increase their application potential as nano-biocatalysts.…”
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“…We have generated a caged SpyCatcher003, which allows light triggering of covalent bond formation to SpyTag003 in mammalian cells. …”
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“…The systematic error and standardized variability of ball-bat contact on the bat coordinate system and pitcher-to-catcher direction when hitting a ball launched from a pitching machine were measured with or without visual occlusion and analyzed using analysis of variance. …”
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