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2881Publicado 1991“…Since the interaction of human CD8 with HLA class I is species specific, we substituted nonconservative amino acids from mouse CD8 and analyzed the ability of the mutated CD8 molecules expressed in COS 7 cells to bind HLA class I-bearing B lymphoblastoid cells, UC. …”
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2882Publicado 1992“…Other cDNA clones encode molecules having diversified cytoplasmic domains. COS7 cells transfected with the cDNA expressed a approximately 60-kD protein and bound IL-5 with a single class of affinity (Kd = 250-590 pM). …”
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2883Isoforms of Ankyrin-3 That Lack the NH(2)-terminal Repeats Associate with Mouse Macrophage Lysosomes“…Also, epitope-tagged mouse macrophage ankyrin-3, transiently expressed in COS cells, associates with intracellular, not plasma, membranes. …”
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2884por Qiao, Hui, Pelletier, Sandra L., Hoffman, Lucas, Hacker, Jill, Armstrong, R. Todd, White, Judith M.“…Mutants were expressed in COS or 293T cells and assayed for cell surface expression and structural features as well as for their ability to change conformation and induce fusion at low pH. …”
Publicado 1998
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2885por Hirschberg, Koret, Miller, Chad M., Ellenberg, Jan, Presley, John F., Siggia, Eric D., Phair, Robert D., Lippincott-Schwartz, Jennifer“…They carried up to 10,000 VSVG–GFP molecules and had a mean life time in COS cells of 3.8 min. In addition, they fused with the plasma membrane without intersecting other membrane transport pathways in the cell. …”
Publicado 1998
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2886por Michiels, Frits, Stam, Jord C., Hordijk, Peter L., van der Kammen, Rob A., Stalle, Lisette Ruuls-Van, Feltkamp, Constance A., Collard, John G.“…Here we show that both in fibroblasts and COS cells, membrane localization of Tiam1 is required for the induction of membrane ruffling. …”
Publicado 1997
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2887por Okada, Akiko, Tomasetto, Catherine, Lutz, Yves, Bellocq, Jean-Pierre, Rio, Marie-Christine, Basset, Paul“…The expression kinetics of these MMP genes, the tissue distribution of their transcripts, the results of cotransfection experiments in COS-1 cells, and zymographic analyses performed using microdissected rat wound tissues support the possibility that during cutaneous wound healing pro-GelA and pro-gelatinase B are activated by MT1-MMP and stromelysin 1, respectively. …”
Publicado 1997
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2888“…Pleckstrin induced cell spreading in both transformed (COS-1 & CHO) and nontransformed (REF52) cell lines, and this spreading was regulated by pleckstrin phosphorylation. …”
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2889por Kazarov, Alexander R., Yang, Xiuwei, Stipp, Christopher S., Sehgal, Bantoo, Hemler, Martin E.“…Presence of the CD151–QRD(194–196)→INF mutant disrupted α3 and α6 integrin–dependent formation of a network of cellular cables by Cos7 or NIH3T3 cells on basement membrane Matrigel and markedly altered cell spreading. …”
Publicado 2002
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2890Publicado 1996“…Expression cloning was done by transient expression in COS-7 cells and immunological selection to isolate a cDNA clone encoding Ag 131/315. …”
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2891por Craig Morton, H., van Zandbergen, Ger, van Kooten, Cees, Howard, Chris J., van de Winkel, Jan G. J., Brandtzaeg, Per“…Chimeras constructed by exchanging complete domains between these two receptors were transfected to COS-1 cells and assayed for their ability to bind hIgA- or bIgG2-coated beads. …”
Publicado 1999
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2892por Uchida, Hiroshi, Kondo, Akiko, Yoshimura, Yasunori, Mazaki, Yuichi, Sabe, Hisataka“…Other ubiquitously expressed ARFGAPs, G protein–coupled receptor kinase interactors GIT2 and GIT2-short/KIAA0148, which we have shown to exhibit GAP activity for ARF1 in COS-7 cells, did not accumulate at the phagocytic cups or inhibit phagocytosis. …”
Publicado 2001
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2893por Ramos, Jorge, Jung, Wonyong, Ramos-Franco, Josefina, Mignery, Gregory A., Fill, Michael“…These chimeric proteins were expressed in COS cells, isolated, and then reconstituted in proteoliposomes. …”
Publicado 2003
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2894Publicado 1991“…These antibodies specifically immunoprecipitated and recognized by Western blotting RAR-gamma 1 proteins in COS-1 cells transfected with expression vectors containing the RAR-gamma 1 cDNAs. …”
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2895Publicado 1991“…Transient expression of the alpha-mannosidase II cDNA in COS cells resulted in 8-12-fold overexpression of enzyme activity, and the appearance of cross-reactive material in a perinuclear membrane array consistent with a Golgi localization. …”
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2896Publicado 1992“…Western blotting of fractions from COS cells transfected with plasmids encoding wild-type and mutant proteins revealed that PKC alpha resided entirely in a Triton X-100 soluble (TS) fraction, whereas both the myristoylated and nonmyristoylated mutants were associated primarily with the nuclear envelope fraction. …”
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2897Publicado 1992“…A linkage between ICAM-1 and cytoskeletal elements was suggested by studies showing a different ICAM-1 staining pattern for COS cells transfected with wild-type ICAM-1 or with an ICAM-1 construct that replaces the cytoplasmic and transmembrane domains of ICAM-1 with a glycophosphatidylinositol (GPI) anchor. …”
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2898Publicado 1991“…Several alpha 4 antigenic determinants were identified on COS-7 cells after transfection. From overlapping clones, approximately 5 kb of contiguous nucleotide sequence have been determined, encoding a protein sequence of 1039 amino acids for the LPAM alpha chain (alpha 4m). …”
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2899“…Ebp1, a protein cloned via its association with the ErbB3 receptor, binds the AR and inhibits androgen-regulated transactivation of wild-type AR in COS cells. As the complement of coregulators in different cells are important for AR activity, we determined the effect of Ebp1 on AR function in prostate cancer cell lines. …”
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2900por Hattori, Takako, Coustry, Francoise, Stephens, Shelley, Eberspaecher, Heidi, Takigawa, Masaharu, Yasuda, Hideyo, de Crombrugghe, Benoit“…Ectopic expression of Sox9 and transient-cotransfection with Tip60 in COS7 cells showed a more diffuse subnuclear colocalization, suggesting changes in the chromatin structure. …”
Publicado 2008
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