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  1. 2901
    “…The effect of the mutation on splicing was examined by exon trapping in COS-7 cells and by RT-PCR on RNA isolated from whole blood. …”
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    Novel de novo BRCA2 mutation in a patient with a family history of breast cancer
  2. 2902
    “…Furthermore, the C/G for wild-type, C/A or T/G for single base variant and T/A for haplotype variant hMYH cDNAs with a flag epitope tag were cloned into pcDNA3.1+ vector and transfected into cos-7 cell line. Their subcellular localizations were determined by immunofluorescence assay. …”
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    A haplotype variation affecting the mitochondrial transportation of hMYH protein could be a risk factor for colorectal cancer in Chinese
  3. 2903
    “…Nevertheless, eIF4F components are indispensable for ASFV protein synthesis and virus spread, since eIF4E or eIF4G depletion in COS-7 or Vero cells strongly prevents accumulation of viral proteins and decreases virus titre. …”
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    Regulation of Host Translational Machinery by African Swine Fever Virus
  4. 2904
    “…To determine if additional ERα phosphorylation sites exist, COS-1 cells expressing human ERα were labeled with [(32)P]H(3)PO(4 )in vivo and ERα tryptic phosphopeptides were isolated to identify phosphorylation sites. …”
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    Identification of four novel phosphorylation sites in estrogen receptor α: impact on receptor-dependent gene expression and phosphorylation by protein kinase CK2
  5. 2905
    “…By quantitative fluorescence microscopy analysis, we demonstrate that soluble EGFP-LC3 is indeed enriched in the nucleus relative to the cytoplasm in two commonly studied cell lines, COS-7 and HeLa. Although LC3 contains a putative nuclear export signal (NES), inhibition of active nuclear export or mutation of the NES had no effect on the nucleo-cytoplasmic distribution of EGFP-LC3. …”
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    Nucleocytoplasmic Distribution and Dynamics of the Autophagosome Marker EGFP-LC3
  6. 2906
    “…Transient expression of cDNA encoding these variants was performed in COS-1 cells to evaluate β-galactosidase activities. …”
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    Three novel beta-galactosidase gene mutations in Han Chinese patients with GM1 gangliosidosis are correlated with disease severity
  7. 2907
    “…In this paper, we compare rabbit antibody responses elicited with multi-allele vaccines incorporating seven (three DiCos and four natural AMA1 alleles) and three (DiCo mix) antigens for gains in broadened specificity. …”
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    Generation of Humoral Immune Responses to Multi-Allele PfAMA1 Vaccines; Effect of Adjuvant and Number of Component Alleles on the Breadth of Response
  8. 2908
    “…Two web-based online resources were built to make these COs available to the scientific community: the ‘CO Lookup Service’ for browsing the CO; and the ‘Crops Terminizer’, an ontology text mark-up tool. …”
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    Multifunctional crop trait ontology for breeders' data: field book, annotation, data discovery and semantic enrichment of the literature
  9. 2909
    “…Using wild-type or mutant over-expression constructs transfected into PC12 and COS-7 cells, the cellular and molecular consequences of the mutations were investigated. …”
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    A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy
  10. 2910
    “…The cysteine mutants were expressed in COS-1 cells and rhodopsin was purified after regeneration with 11-cis-retinal. …”
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    The Roles of Transmembrane Domain Helix-III during Rhodopsin Photoactivation
  11. 2911
    “…EXPERIMENTAL APPROACH: Transfected mouse atrial cardiomyocytes (HL-1 cells) and COS7 cells were subjected to luciferase reporter gene assay and whole-cell patch clamp. …”
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    Transcriptional activation of the anchoring protein SAP97 by heat shock factor (HSF)-1 stabilizes K(v)1.5 channels in HL-1 cells
  12. 2912
    “…RESULTS: In our study, we detected cytoplasmic protein aggregates by expressing GFP-tagged NTR in COS-7 cells, suggesting an impaired translation by divergent codon usage between prokaryotes and eukaryotes. …”
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    A mammalianized synthetic nitroreductase gene for high-level expression
  13. 2913
    “…Furthermore, we transiently transfected COS-7 cells with each of gene constructs that express five human CD45 isoforms respectively, and examined the specificities of the mAbs against the transfected isoforms. …”
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    Characterization of Monoclonal Antibodies against Human Leukocyte Common Antigen (CD45)
  14. 2914
    “…The list of siRNA seats is available through a publicly available database located at http://web.cos.gmu.edu/~gmanyam/siRNA_db/search.php CONCLUSIONS: In attempt to perform top-down prediction of the human siRNA with minimized off-target hybridization, we developed an efficient algorithm that employs suffix tree based storage of the substrings. …”
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    An efficient algorithm for systematic analysis of nucleotide strings suitable for siRNA design
  15. 2915
    “…During a two staged sub-maximal bicycle exercise test conducted at 4 and 16 weeks of implant, COs measured by direct Fick technique and estimated by the ICD were recorded and compared. …”
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    Estimation of cardiac output in patients with congestive heart failure by analysis of right ventricular pressure waveforms
  16. 2916
    “…METHODS: Transcription reporter assays using Cos7 cells were performed to determine if specific sulfonylureas stimulate PPARγ transactivation. …”
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    Effects of Sulfonylureas on Peroxisome Proliferator-Activated Receptor γ Activity and on Glucose Uptake by Thiazolidinediones
  17. 2917
    “…Over-expression of p110γ in Cos7 cells resulted in decreased acidification of CpG containing endosome. …”
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    Phosphoinositide 3-Kinaseγ Controls the Intracellular Localization of CpG to Limit DNA-PKcs-Dependent IL-10 Production in Macrophages
  18. 2918
    “…The eight markers were successful in uniquely identifying sixty-two vervet monkey DNA samples and confirmed that Vero76 cells and COS-7 cells were derived from Vero and CV-1 cells, respectively. …”
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    Authentication of African green monkey cell lines using human short tandem repeat markers
  19. 2919
    “…The distal translational start site is functional and promotes RNase 8 synthesis in transfected COS-7 cells. CONCLUSIONS: These results suggest that RNase 8 may diverge considerably from typical RNase A family ribonucleases and may likewise exhibit unique function. …”
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    Genetic diversity of human RNase 8
  20. 2920
    “…Moreover, MSM decreased the DNA-binding activities of STAT5b and STAT3, to the target gene promoters in MDA-MB 231 or co-transfected COS-7 cells. We confirmed that MSM significantly decreased the relative luciferase activities indicating crosstalk between STAT5b/IGF-1R, STAT5b/HSP90α, and STAT3/VEGF. …”
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    Methylsulfonylmethane Suppresses Breast Cancer Growth by Down-Regulating STAT3 and STAT5b Pathways
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