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3081por Drummond, Marcela G., Calzavara-Silva, Carlos E., D'Astolfo, Diego S., Cardoso, Fernanda C., Rajão, Matheus A., Mourão, Marina M., Gava, Elisandra, Oliveira, Sérgio C., Macedo, Andréa M., Machado, Carlos R., Pena, Sérgio D. J., Kitten, Gregory T., Franco, Glória R.“…Also, SmZF1 was heterologously expressed in mammalian COS-7 cells to produce the recombinant protein YFP-SmZF1, which was mainly detected in the nucleus of the cells by confocal microscopy and Western blot assays. …”
Publicado 2009
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3082por Conklin, Matthew W, Ada-Nguema, Aude, Parsons, Maddy, Riching, Kristin M, Keely, Patricia J“…RESULTS: GFP-R-Ras localized to the plasma membrane, most specifically in membrane ruffles, in Cos-7 cells. GFP-R-Ras was endocytosed from these ruffles, and trafficked via multiple pathways, one of which involved large, acidic vesicles that were positive for Rab11. …”
Publicado 2010
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3083por Panebra, Alfredo, Wang, Wayne C., Malone, Molly M., Pitter, Demar R. G., Weiss, Scott T., Hawkins, Gregory A., Liggett, Stephen B.“…“Whole-gene” transfections were performed with COS-7 cells and revealed 4 haplotypes with increased cell surface β(2)AR protein expression compared to the others. …”
Publicado 2010
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3084por Bader, Hannah L., Ruhe, Alison L., Wang, Lauren W., Wong, Aaron K., Walsh, Kari F., Packer, Rebecca A., Mitelman, Jonathan, Robertson, Kathryn R., O'Brien, Dennis P., Broman, Karl W., Shelton, G. Diane, Apte, Suneel S., Neff, Mark W.“…Murine ADAMTSL2 containing the p.R221C mutation formed anomalous disulfide-bonded dimers when transiently expressed in COS-1, HEK293F and CHO cells, and was present in the medium of these cells at lower levels than wild-type ADAMTSL2 expressed in parallel. …”
Publicado 2010
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3085“…We validated the interaction in vitro and in transfected COS-7 cells. We further confirmed the interaction of endogenous Rasd1 and Ear2 from HEK293T cell and mouse brain extract. …”
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3086“…AhR up-regulated αB-crystallin promoter activity in transfected HeLa, NIH3T3 and COS-7 cells in the absence of exogenously added ligand (TCDD), but had no effect on the αB-crystallin promoter in C(2)C(12), CV-1 or Hepa-1 cells with or without TCDD. …”
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3087“…We used confocal microscopy and fluorescence recovery after photobleaching to define these properties for each IP(3)R subtype expressed heterologously in COS-7 cells. IP(3)R1 and IP(3)R3 were uniformly distributed within the membranes of the endoplasmic reticulum (ER), but the distribution of IP(3)R2 was punctate. …”
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3088“…Previous studies in transfected COS cells overexpressing G protein coupled receptor kinases (GRKs) demonstrated that GRK2, GRK3, GRK5 and GRK6 participate in agonist-induced C3aR phosphorylation. …”
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3089por Looi, Chung Yeng, Imanishi, Miki, Takaki, Satoshi, Sato, Miki, Chiba, Natsuko, Sasahara, Yoji, Futaki, Shiroh, Tsuchiya, Shigeru, Kumaki, Satoru“…In contrast, WT Lnk R8 showed no growth inhibitive effect on non-hematopoietic HELA or COS-7 cell. Moreover, we demonstrated that TPO-induced M-MOK cell growth inhibition by WT Lnk R8 was dose-dependent. …”
Publicado 2011
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3090por Bedoyan, Jirair Krikor, Schaibley, Valerie M, Peng, Weiping, Bai, Yongsheng, Mondal, Kajari, Shetty, Amol C, Durham, Mark, Micucci, Joseph A, Dhiraaj, Arti, Skidmore, Jennifer M, Kaplan, Julie B, Skinner, Cindy, Schwartz, Charles E, Antonellis, Anthony, Zwick, Michael E, Cavalcoli, James D, Li, Jun Z, Martin, Donna M“…Western analysis and fluorescence microscopy of GFP-tagged RAB40AL constructs from transiently transfected COS7 cells show that the D59G missense change renders RAB40AL unstable and disrupts its cytoplasmic localisation. …”
Publicado 2012
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3091por Elmabsout, Ali Ateia, Kumawat, Ashok, Saenz-Méndez, Patricia, Krivospitskaya, Olesya, Sävenstrand, Helena, Olofsson, Peder S., Eriksson, Leif A., Strid, Åke, Valen, Guro, Törmä, Hans, Sirsjö, Allan“…The spliced CYP26B1 still has the capability to degrade atRA, but at an initial rate one-third that of the corresponding full length enzyme. Transfection of COS-1 and THP-1 cells with the CYP26B1 spliced variant indicated either an increase or a decrease in the catabolism of atRA, probably depending on the expression of other atRA catabolizing enzymes in the cells. …”
Publicado 2012
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3092por Jung, Hyun Jun, Lim, Jung-Suk, Choi, Hyo-Jung, Lee, Mi Suk, Kim, Jong-Ho, Kim, Sang-Yeob, Kim, Soyoun, Kim, Eunjung, Kwon, Tae-Hwan“…The results revealed that 1) synthesized dDAVP-9r peptides formed a stable polyplex with siRNA; 2) siRNA/dDAVP-9r polyplex could bind to the V2R of IMCD cells and induced AQP2 phosphorylation (Ser 256); 3) siRNA/dDAVP-9r polyplex was stable in response to the wide range of different osmolalities, pH levels, or to the RNases; 4) fluorescein-labeled siRNA was delivered into V2R-expressing MDCK and LLC-PK1 cells by siRNA/dDAVP-9r polyplex, but not into the V2R-negative Cos-7 cells; and 5) AQP2-siRNA/dDAVP-9r polyplex effectively delivered siRNA into the IMCD cells, resulting in the significant decrease of protein abundance of AQP2, but not AQP4. …”
Publicado 2012
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3093por Esapa, Christopher T., Head, Rosie A., Jeyabalan, Jeshmi, Evans, Holly, Hough, Tertius A., Cheeseman, Michael T., McNally, Eugene G., Carr, Andrew J., Thomas, Gethin P., Brown, Matthew A., Croucher, Peter I., Brown, Steve D. M., Cox, Roger D., Thakker, Rajesh V.“…Transient transfection of wild-type and mutant Galnt3-enhanced green fluorescent protein (EGFP) constructs in COS-7 cells revealed endoplasmic reticulum retention of the Trp589Arg mutant and Western blot analysis of kidney homogenates demonstrated defective glycosylation of Galnt3 in Tcal/Tcal mice. …”
Publicado 2012
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3094por Acharya, Moulinath, Sharp, Michael W., Mirzayans, Farideh, Footz, Tim, Huang, LiJia, Birdi, Chanchal, Walter, Michael A.“…Co-immunoprecipitation assays in COS-7 cells confirmed the interaction between PITX2 and EFEMP2. …”
Publicado 2012
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3095por Hanchate, Naresh Kumar, Giacobini, Paolo, Lhuillier, Pierre, Parkash, Jyoti, Espy, Cécile, Fouveaut, Corinne, Leroy, Chrystel, Baron, Stéphanie, Campagne, Céline, Vanacker, Charlotte, Collier, Francis, Cruaud, Corinne, Meyer, Vincent, García-Piñero, Alfons, Dewailly, Didier, Cortet-Rudelli, Christine, Gersak, Ksenija, Metz, Chantal, Chabrier, Gérard, Pugeat, Michel, Young, Jacques, Hardelin, Jean-Pierre, Prevot, Vincent, Dodé, Catherine“…Seven mutations resulted in impaired secretion of semaphorin-3A by transfected COS-7 cells (D538fsX31, R66W, V435I) or reduced signaling activity of the secreted protein in the GN11 cell line derived from embryonic GnRH cells (N153S, I400V, T688A, R733H), which strongly suggests that these mutations have a pathogenic effect. …”
Publicado 2012
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3096por Sun, Qiangzheng, Lan, Ruiting, Wang, Yiting, Wang, Jianping, Wang, Yan, Li, Peijing, Du, Pengcheng, Xu, Jianguo“…SfI was found to use a cos mechanism for DNA packaging similar to that of phage SfV. …”
Publicado 2013
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3097por Wang, Weiwei, Li, Wenzhong, Ong, Lee-Lee, Furlani, Dario, Kaminski, Alexander, Liebold, Andreas, Lützow, Karola, Lendlein, Andreas, Wang, Jun, Li, Ren-Ke, Steinhoff, Gustav, Ma, Nan“…Optimization of GAC was carried out based on collagen effects on the PEI/DNA complexes, viability and luciferase expression of COS7 cells on GAC. CD117(+) stem cells homing in response to SDF-1α local expression from transfected cells on GAC were investigated in a flow chamber in vitro and in a mouse hind limb model in vivo. …”
Publicado 2010
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3098“…The recombinant molecules along with wild type fV (fV(WT)) were transiently expressed in COS-7L cells, purified, and assessed for their ability to bind factor Xa (fXa) prior to and following incubation with thrombin. …”
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3099por Ashour, Mohamed L, El-Readi, Mahmoud Z, Hamoud, Razan, Eid, Safaa Y, El Ahmady, Sherweit H, Nibret, Endalkachew, Herrmann, Florian, Youns, Mahmoud, Tahrani, Ahmed, Kaufmann, Dorothea, Wink, Michael“…Cytotoxicity was investigated in eight cancer cell lines (Caco-2, CCL-81, CCRF-CEM, COS-7, HL-60, MIA PaCa-2, MCF-7, and PANC-1) using the MTT assay and the caspase 3/7 activity was determined over the range of 62.5–1000 μg/mL. …”
Publicado 2014
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3100por Ramsauer, Brigitte, Lotzin, Annett, Mühlhan, Christine, Romer, Georg, Nolte, Tobias, Fonagy, Peter, Powell, Bert“…METHODS/DESIGN: This randomized controlled clinical trial tests whether promoting attachment security in infancy with the Circle of Security (COS) Intervention will result in a higher rate of securely attached children compared to treatment as usual (TAU). …”
Publicado 2014
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