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Mostrando 281 - 300 Resultados de 1,123 Para Buscar '"Firefly"', tiempo de consulta: 0.33s Limitar resultados
  1. 281
    “…Similar potency-enhancing effects were observed as this approach was applied to other functional siRNAs targeting a different site on the firefly luciferase transcript or endogenously expressed PTEN.…”
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    Modulation of thermal stability can enhance the potency of siRNA
  2. 282
    “…As a proof of concept, we investigated knockdown of the firefly luciferase reporter gene by combinatorial use of RNAi and U1i, and evaluated their inhibitory potential both in vitro and in vivo. …”
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    AAV-mediated in vivo knockdown of luciferase using combinatorial RNAi and U1i
  3. 283
    “…Here, we develop a novel dual luciferase reporter pull-down assay by combining a biotinylated Firefly luciferase pull-down assay with a dual luciferase reporter assay. …”
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    Relative Quantification of Protein-Protein Interactions Using a Dual Luciferase Reporter Pull-Down Assay System
  4. 284
    “…Currently, only two luciferases (Firefly and Renilla) are commonly used without substrate cross-talk. …”
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    Expanding luciferase reporter systems for cell-free protein expression
  5. 285
    “…The transformants were successfully grown on Tp antibiotic plates, and firefly luciferase expression was determined by measuring the relative light unit (RLU). …”
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    Optimization of Constitutive Promoters Using a Promoter-Trapping Vector in Burkholderia pyrrocinia JK-SH007
  6. 286
    “…The three most successful algorithms in both training and testing processes are the firefly algorithm, selfish herd optimizer, and grasshopper optimization algorithm, respectively. …”
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    Training of Feed-Forward Neural Networks by Using Optimization Algorithms Based on Swarm-Intelligent for Maximum Power Point Tracking
  7. 287
    “…In vivo, antiglioma activity of VA7 was tested in Balb/c nude mice using U87 cells stably expressing firefly luciferase in subcutaneous and orthotopic tumor models. …”
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    Intravenously Administered Alphavirus Vector VA7 Eradicates Orthotopic Human Glioma Xenografts in Nude Mice
  8. 288
    “…The results showed that the accuracy and computational speed of the proposed method were better than the existing Differential Evolution, Firefly Algorithm and Chemical Reaction Optimization methods. …”
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    An Improved Swarm Optimization for Parameter Estimation and Biological Model Selection
  9. 289
    “…The strategy is to reasonably split the gene of firefly luciferase protein into two inactive fragments and then respectively fuse the two fragments to Rho GTPase and the GTPase-binding domain (GBD) of the specific effector. …”
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    Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases
  10. 290
    “…These cells were cultured, transduced to express eGFP and firefly luciferase, and gave rise to a highly aggressive, transplantable B cell lymphoma cell line, termed IM380. …”
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    Non-Invasive Bioluminescence Imaging to Monitor the Immunological Control of a Plasmablastic Lymphoma-Like B Cell Neoplasia after Hematopoietic Cell Transplantation
  11. 291
    “…We report a CRISPR/Cas9-based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the control of endogenous promoter, using the TGFβ-responsive gene PAI-1. …”
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    Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9
  12. 292
    “…RESULTS: In this study, bioluminescent composites comprised of magnetic nanoparticles and firefly luciferase (Photinus pyralis) are examined as potential light-emitting agents for imaging, detection, and tracking mammalian spermatozoa. …”
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    Bioluminescent magnetic nanoparticles as potential imaging agents for mammalian spermatozoa
  13. 293
    “…Two amplification refractory mutation systems (cobas-ARMS and ADx-ARMS), a droplet digital polymerase chain reaction (ddPCR) and a next-generation sequencing (Firefly NGS) platform were included in the comparison. …”
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    Cross-Platform Comparison of Four Leading Technologies for Detecting EGFR Mutations in Circulating Tumor DNA from Non-Small Cell Lung Carcinoma Patient Plasma
  14. 294
    “…The presented method combines transient expression via agroinfiltration of Nicotiana benthamiana leaves with a quantitative dual luciferase reporter system, where firefly luciferase is used to report the microRNA-target sequence interaction and Renilla luciferase is used as an internal standard to normalize expression between replicates. …”
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    An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences
  15. 295
    “…This area of work has been dominated by firefly luciferase and its substrate, D-luciferin, due to the system’s peak emission (~ 600 nm), high signal to noise ratio, and generally favorable biodistribution of D-luciferin in mice. …”
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    Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging
  16. 296
    “…We also recovered a predicted homolog of 4-coumarate-CoA ligase (268 amino acids in length) that best matched luciferase of the firefly Luciola with the best predicted template being the crystal structure of luciferase for Photinus pyralis, the common eastern firefly. …”
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    The transcriptome of the Bermuda fireworm Odontosyllis enopla (Annelida: Syllidae): A unique luciferase gene family and putative epitoky-related genes
  17. 297
    “…EBV–firefly luciferase was detectable 1 week after infection by noninvasive optical imaging in the spleen, from where it spread rapidly and systemically. …”
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    Spatiotemporally Skewed Activation of Programmed Cell Death Receptor 1–Positive T Cells after Epstein-Barr Virus Infection and Tumor Development in Long-Term Fully Humanized Mice
  18. 298
    “…Here, we report a biochemiluminescent sialidase assay that uses a substrate derivatized with firefly luciferin. In the presence of sialidase in the reaction, the substrate is cleaved to release luciferin, which is subsequently oxidized by firefly luciferase to generate a light signal. …”
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    A Biochemiluminescent Sialidase Assay for Diagnosis of Bacterial Vaginosis
  19. 299
    “…The detection of transgenic parasites expressing wildtype firefly luciferase is however hampered by a relatively low and heterogeneous tissue penetrating capacity of emitted light. …”
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    Comparison of Bioluminescent Substrates in Natural Infection Models of Neglected Parasitic Diseases
  20. 300
    “…Targeting different developmental stages, we investigated the utility of soaking and/or square wave electroporation in order to transfect S. haematobium with nucleic acid reporters including Cy3-labeled small RNAs, messenger RNA encoding firefly luciferase, and short interfering RNAs (siRNAs). …”
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    Genetic Manipulation of Schistosoma haematobium, the Neglected Schistosome
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