“…We compare maCLuc with several established luciferase-based reporter systems (firefly, click beetle, Renilla, and Gaussia luciferases), using both in vitro and in vivo models. …”
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“…This approach is composed of two different phases in which are applied two metaheuristic algorithms, respectively the Firefly (FA) and the Artificial Bee Colony (ABC) algorithms. …”
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“…In this study, a Random Forests (RF) and Firefly Algorithm (FA) hybrid machine learning model was proposed to predict the compressive strength of metakaolin cement-based materials. …”
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“…In recent da Vinci robot systems (Intuitive Surgical), near-infrared fluorescence imaging (Firefly technology) has been used. With this technique, it is possible for surgeons to see important structures well beyond the limits of their eyes; hence, surgeons can safely operate and make critical decisions accurately using image-guided surgery. …”
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“…For the estimation of viscosity, the firefly algorithm (FA) was employed to optimize the hyper-parameters of the machine learning models. …”
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“…Then, review features are extracted by the genetic algorithm (GA), and the optimal review feature set is extracted using the firefly algorithm (FA). Finally, the LSTM-DGWO model categorizes app reviews, and the DGWO algorithm optimizes the hyperparameters of the LSTM model. …”
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“…In vivo experiments demonstrated that the best C4S18A formulations, which embody linoleoyl tails, show strong firefly luciferase (Fluc) mRNA expression in the liver and spleen via intravenous (IV) injection, or at the local injection site via intramuscular injection (IM). …”
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“…Each CPM is equipped with 8 Samtec FireFly modules connected to a Xilinx Kintex UltraScale FPGA for data buffering, online digital processing and on-detector electronics control. …”
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“…Pseudotype entry was detected by the expression of firefly luciferase or green fluorescent protein (gfp). …”
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“…We found that the FLUX(Vc) reporter gene can be overexpressed in various cell lines and showed outstanding signal-to-background in HepG2 cells, significantly higher than that of firefly luciferase (Fluc). The combined use of FLUX(Vc)/Fluc as target/control vectors gave the most stable signals, better than the standard set of Fluc(target)/Rluc(control). …”
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“…Here, we describe the development and validation of a reporter gene assay (RGA) based on the engineered C8166-STAT5RE-Luc cells expressing endogenous IL-2 receptors and a STAT5-inducible element-driven firefly luciferase in C8166 cell lines. The RGA was fully validated according to the International Conference on the Harmonization of Technical Requirements for the Registration of Pharmaceuticals for the Human Use-Q2 (ICH-Q2). …”
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“…The widespread use of the pRF vector is a major problem because this vector, which has Renilla luciferase as the 5′ cistron and firefly luciferase as the 3′ cistron, has been found to generate spliced transcripts. …”
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“…Using noninvasive bioluminescent imaging and a mathematical model of siRNA delivery and function, the effects of target-specific and treatment-specific parameters on siRNA-mediated gene silencing are monitored in cells stably expressing the firefly luciferase protein. In vitro, luciferase protein levels recover to pre-treatment values within <1 week in rapidly dividing cell lines, but take longer than 3 weeks to return to steady-state levels in nondividing fibroblasts. …”
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“…Using the method, we constructed both single- and multi-amiRNA expression vectors to target three reporter genes, which code firefly luciferase (Fluc), enhanced green fluorescent protein (EGFP) and β-galactosidase (LacZ), respectively. …”
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“…We performed an adoptive transfer of luciferase-specific T cells, isolated from spleen or lung of donor mice injected with VSV-G-pseudotyped lentivirus vector expressing luciferase into the muscle or lung, respectively, into recipient recombination-activating gene (RAG)–deficient mice transduced in lung with adenovirus expressing firefly luciferase (ffluc2). Gene expression declined within 7 days of adoptive transfer approaching background levels by day 36. …”
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“…We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. …”
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“…For example, how did signals such as the tail-fan display of a peacock, a firefly flash or a wolf howl evolve? Animal communication theory holds that many signals evolved from non-signalling behaviours through the process of ritualization. …”
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“…RESULTS: Using transgenic mice expressing firefly luciferase under the regulation of the GFAP promoter (GFAP-luc), we developed a model system to monitor astrogliosis upon tumor growth in a rapid, non-invasive manner. …”
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“…In this paper, a transgenic mouse line, B6-Tg(c-Rel-luc)(Mlit), which incorporated the transgene firefly luciferase driven by a 14.5-kb fragment containing mouse c-Rel gene Rel promoter, was generated to monitor Rel expression in vivo. …”
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“…We present an optimized triple modality reporter construct combining a far-red fluorescent protein (E2-Crimson), enhanced firefly luciferase enzyme (Luc2), and truncated wild type herpes simplex virus I thymidine kinase (wttk) that allows for sensitive, long-term tracking of tumor growth in vivo by fluorescence, bioluminescence, and positron emission tomography. …”
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