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401por Hsieh, Tzung-yang, Nillegoda, Nadinath B., Tyedmers, Jens, Bukau, Bernd, Mogk, Axel, Kramer, Günter“…Here we use a plasmid encoded orthogonal tRNA/aminoacyl-tRNA synthetase pair to site-specifically label firefly luciferase with the environmentally sensitive fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2- aminopropanoic acid (ANAP) and explore the detectability of conformational changes in labeled luciferase in the yeast cytoplasm. …”
Publicado 2014
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402por Holvoet, Bryan, Quattrocelli, Mattia, Belderbos, Sarah, Pollaris, Lore, Wolfs, Esther, Gheysens, Olivier, Gijsbers, Rik, Vanoirbeek, Jeroen, Verfaillie, Catherine M., Sampaolesi, Maurilio, Deroose, Christophe M.“…Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). …”
Publicado 2015
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403por Bojilova, Ekaterina Dimitrova, Weyn, Christine, Antoine, Marie-Hélène, Fontaine, Véronique“…HDACi strongly induced transcription of the firefly luciferase reporter gene under the control of the HPV-16 LCR in a variety of cell lines. …”
Publicado 2016
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404por Lopez-Franco, Carlos, Gomez-Avila, Javier, Alanis, Alma Y., Arana-Daniel, Nancy, Villaseñor, Carlos“…For the purpose of showing the effectiveness of the sensor integration and control algorithm to address these issues on a high nonlinear system with noisy sensors as cameras, experiments were performed on the Asctec Firefly on-board computer, including both simulation and experimenta results.…”
Publicado 2017
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405“…In circadian rhythms research, for example, clock gene fusions with firefly luciferase give rise to robust rhythms in cellular bioluminescence that persist over many days. …”
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406“…Using the circularly permuted form of firefly luciferase, we have developed a reporter for Caspase 3/7, referred to as Caspase 3/7 GloSensor. …”
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407por Gerace, Dario, Martiniello-Wilks, Rosetta, Habib, Rosaline, Simpson, Ann Margaret“…Due to the functional requirement of ATP for the Firefly luciferase gene Luc2 to produce light, luciferase-based reporting of cytotoxicity can be engineered into potential cell therapies. …”
Publicado 2019
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408“…Three statistical tests conducted on BSA compared to differential evolution algorithm (DE), particle swarm optimisation (PSO), artificial bee colony (ABC), and firefly algorithm (FF). The tests are used to evaluate these mentioned algorithms and to determine which one could solve a specific optimisation problem concerning the statistical success of 16 benchmark problems taking several criteria into account. …”
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409por Rameix-Welti, Marie-Anne, Le Goffic, Ronan, Hervé, Pierre-Louis, Sourimant, Julien, Rémot, Aude, Riffault, Sabine, Yu, Qin, Galloux, Marie, Gault, Elyanne, Eléouët, Jean-François“…Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. …”
Publicado 2014
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410“…Furthermore, extensive experiments validate the superiority of the whale optimization algorithm over the other four swarm intelligence optimization algorithms (gray wolf optimizer, fruit fly optimization algorithm, particle swarm optimization, and firefly algorithm) through various indicators of the results, especially under harsh constraints.…”
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411por Wang, Bei, Zhang, Chongyang, Lei, Xiaobo, Ren, Lili, Zhao, Zhendong, Wang, Jianwei, Huang, He“…Here, we report the construction of plasmid-based, non-infectious SARS-CoV-2 replicons with turbo-green fluorescent protein and/or firefly luciferase reporters by reverse genetics using transformation-associated recombination cloning in Saccharomyces cerevisiae. …”
Publicado 2021
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412“…The proposed solution employs the Firefly algorithm to optimize the process of the selection-offloading computing mode and determine the optimal solution for performing tasks locally or offloaded to a fog or cloud considering the task dependency. …”
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413por Engel, Laurie, Alves, Juliano, Hennek, Jacquelyn, Goueli, Said A., Zegzouti, Hicham“…Each of these assays are performed in a one-step detection that relies on converting the nucleotide product to ATP, then to bioluminescence using firefly luciferase. These assays are highly sensitive, robust and resistant to chemical interference. …”
Publicado 2021
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414por Tate, Sophia J., Van de Sande, Leen, Ceelen, Wim P., Torkington, Jared, Parker, Alan L.“…Methods: Adenoviral vectors expressing reporter genes green fluorescence protein (Ad5.GFP) or firefly luciferase (Ad5.Luc) were subject to pressurised aerosolisation. …”
Publicado 2021
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415por Muramatsu, Hiromi, Lam, Kieu, Bajusz, Csaba, Laczkó, Dorottya, Karikó, Katalin, Schreiner, Petra, Martin, Alan, Lutwyche, Peter, Heyes, James, Pardi, Norbert“…Importantly, we show in comparative mouse studies that lyophilized firefly luciferase-encoding mRNA-LNPs maintain their high expression, and no decrease in the immunogenicity of a lyophilized influenza virus hemagglutinin-encoding mRNA-LNP vaccine was observed after 12 weeks of storage at room temperature or for at least 24 weeks after storage at 4°C. …”
Publicado 2022
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416por Rinesh, S., Maheswari, K., Arthi, B., Sherubha, P., Vijay, A., Sridhar, S., Rajendran, T., Waji, Yosef Asrat“…The value of k in both methods is determined using the optimization process called the firefly algorithm. The optimization processes reduce the manual calculation for finding K's optimal value to segment the brain regions. …”
Publicado 2022
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417por Cao, Xiangyi, Zhang, Yulong, Zhou, Qianqian, Sun, Sujing, He, Minwei, Wang, Xiaohui, Ma, Ping, Yang, Xiaoang, Lv, Liping, Zhan, Linsheng“…In addition, macrophages expressing arginase-1(Arg1) promoter coupled with firefly luciferase were engineered for bioluminescence imaging (BLI) of the tumor microenvironment. …”
Publicado 2022
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418“…The simulation results obtained after applying the algorithm to the test images show that the proposed approach achieved high-precision and robust registration compared to other methods such as GWO, PSO, Firefly Algorithm (FA), and Crow Searcha Algorithms (CSA).…”
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419A caged imidazopyrazinone for selective bioluminescence detection of labile extracellular copper(ii)“…Unlike the more commonly-used firefly luciferase, nanoluciferase does not require ATP, allowing its application to the extracellular milieu. pic-DTZ demonstrates high metal and oxidation state selectivity for Cu(ii) in aqueous buffer as well as selectivity for labile pools over coordinatively inaccessible protein-bound Cu(ii). …”
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420por Pilipenko, Iuliia, Korovkina, Olga, Gubina, Nina, Ekimova, Viktoria, Ishutinova, Anastasia, Korzhikova-Vlakh, Evgenia, Tennikova, Tatiana, Korzhikov-Vlakh, Viktor“…The poly(lysine-co-isoleucine) polypeptides showed greater than poly(ethyleneimine) efficacy as vectors for transfection of cells with green fluorescent protein and firefly luciferase encoding mRNAs. This allows us to consider obtained copolymers as promising candidates for mRNA delivery applications.…”
Publicado 2022
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